Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis

FAN Jian-hua; DENG Yong-bo; XUAN Ming; ZHOU Song; WU Jun-feng; LIU Yong-shun; WU Yi-hui

doi:10.3788/OPE.20142210.2733

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Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis

更新时间：2020-08-13

- Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis
- Optics and Precision Engineering Vol. 22, Issue 10, Pages: 2733-2739(2014)
- 作者机构：
  
  1. 中国科学院长春光学精密机械与物理研究所,吉林长春,中国,130033
  2. 中国科学院大学北京,中国,100049
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20142210.2733
  CLC： Q-33;TQ022.1
- Received：07 December 2013，
  
  Revised：20 January 2014，
  
  Published：25 October 2014
- 稿件说明：
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范建华, 邓永波, 宣明等. CD-like全血分析芯片内压缩空气的定量泵送[J]. 光学精密工程, 2014,22(10): 2733-2739

FAN Jian-hua, DENG Yong-bo, XUAN Ming etc. Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis[J]. Editorial Office of Optics and Precision Engineering, 2014,22(10): 2733-2739
范建华, 邓永波, 宣明等. CD-like全血分析芯片内压缩空气的定量泵送[J]. 光学精密工程, 2014,22(10): 2733-2739 DOI： 10.3788/OPE.20142210.2733.

FAN Jian-hua, DENG Yong-bo, XUAN Ming etc. Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis[J]. Editorial Office of Optics and Precision Engineering, 2014,22(10): 2733-2739 DOI： 10.3788/OPE.20142210.2733.

摘要

为了克服离心式微流控芯片上血清提取对虹吸管亲水性的依赖

保证离心式微流控芯片功能的稳定性和可靠性

在芯片上设计了连接于血液分离腔的压缩空气腔

实现了高转速下血液的分离.基于压缩空气的辅助作用

并通过转速降低导致压缩空气腔内所储存气体压强的释放对血清的泵送作用

实现了离心式微流控芯片上血清的定量提取.基于等温气体的热力学平衡理论

分析了压缩空气腔压缩体积和虹吸管内液面位置与电机转速之间的关系

给出了基于压缩空气辅助作用的离心式血清提取结构的设计规律.以聚甲基丙烯酸甲酯(PMMA)为基材

采用CO

激光加工工艺

制作了离心式血清提取芯片

并测试了不同转速下被压缩气体的体积和血清液面在虹吸管中的位置.实验结果表明:转速为4000 r/min时

空气的被压缩量为8.7 μL

虹吸管能有效抑制全血溢出以防止全血进入血清提取腔;当转速降为1 000 r/min时

压缩气体所储存压强得到释放

克服了离心力

并驱动血清流过虹吸管最高点

进而实现血清的定量提取.

Abstract

To overcome the dependence of the plasma extraction on siphon hydrophilicity in a centrifugal microfluidic chip and to ensure long-term stability and reliability of the microfluidic chip

a compressed air reservoir connected to the blood separated reservoir was proposed to separate the blood in blood separated reservoir at high rotating speeds. Through lowering the spinning speed

the plasma in the separated reservoir was pumped toward the CD-like chip center to release the stored air pressure produced by the rotation at high spinning speeds. Based on the thermodynamics of the isothermal gas

the pneumatic pumping method was modeled

and the model was confirmed by analyzing pumping positions and air compression versus spinning speeds. By using the layered Polymethyl Methacrylate(PMMA)as materials

the centrifugal microfluidic chips for plasma extraction were fabricated with a CO

laser process technique and the volume of compressed air and the blood position at siphon were tested at different rotational speeds. Experimental results demonstrate that the achieved volume of compression is 8.7μL and the siphon valve effectively inhibits whole blood to overflow the crest of siphon at 4 000 r/min speed. With lowering the spin speed to 1 000 r/min

the release of compressed air overcomes the centrifugal force and drives the plasma to flow past the crest of siphon. Then the plasma extraction is realized in quantitation.

关键词

Keywords

references

AMASIA M,MADOU M. Large-volume centrifugal microfluidic device for blood plasma separation[J].Bioanalysis,2010,2(10):1701-1710.

TIMOTHY A.CROWLEY.VINCENT P. Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications[J]. Lab on a Chip, 2005,5:922-929.

BLATTERT C,JURISCHKA R,TAHHAN I,et al.. Microfluidic Blood/Plasma Separation Unit Based on Microchannel Bend Structures[C]. Proceedings of the 3th conference on microtechnologies in medicine and biology,Hawaii,IEEE,2005:38-41.

SUNG Y,AKIF U,JEFFREY D,et al.. A microfluidic device for continuous, real time blood plasma separation [J]. Lab on a Chip,2006,6:871-880.

STEIGER J,BRENNER M,GRUMANN M,et al..Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a –disk[J].Biomedical Microdevices,2007,9:675-679.

ROBER G,LIVIU C,MARC M. Pneumatic pumping in centrifugal microfluidic platforms[J].Microfluid Nanofluid,2010,9:541-549.

SIEGRIST J,GORKIN R,BASTIEN M,et al..Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples [J].Lab on a Chip,2010,10,363-371.

邓永波,张平,杜新,等. 亲/ 疏水性不同壁面组成微通道的深宽比与通道内液体的自发毛细流动[J]. 光学精密工程 2010,18(7):1562-1567. DENG Y B,ZHANG P,DU X,et al..Aspect ratio for microchannels with nonuniform surface properties and spontaneous capillary[J].Opt. Precision Eng.,2010,18(7):1562-1567.(in Chinese)

杜新,张平,刘永顺,等. 基于PDMS 和玻璃材料的毛细管被动阀临界压力分析[J]. 光学精密工程,2011,19(8):1852-1858. DU X,ZHANG P,LIU Y S,et al..Burst pressure of capillary burst valve based on glass and PDMS[J].Opt.Precision Eng.,2011,19(8):1852-1858.(in Chinese)

STEFAN H,THILO B,ROLAND Z,et al..Centrifugal extraction of plasma from whole blood on a rotating disk [J]. Lab on a Chip,2006,6:776-781.

ROBER B,NUNO R,JOAO G F,et al..Plasma extraction by centrifugo-pneumatically induced gating of flow[J].Journal of Micromechanics and Miroengineering,2013,23:035035.

KITSARA M,DUCREE J.Integration of functional materials and surface modification for polymeric microfluidic systems[J]. Journal of Micromechanics and Miroengineering,2013,23:033001.

LI T J.ZHANG L M,KAR M L,et al..Out-of-plane microvalves for whole blood separation on lab-on-a-CD [J]. Journal of Micromechanics and Miroengineering,2010,20:105024.

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