Different resolution pixels have a series of transmission coefficient

according to this feature of biological specimen

we device a protein model with several differnt high steps.Then calculate the exposure corresponding to the relationships of exposure

ratio of signal to noise

contrast

and performance of resist.We also analogue the development condition of specimen model

measure the resist development speeds of every step

analyze the development condition quantitatively

connect resist development condition with contrast

resolution and exposure very closely

find out a better experiment method.