Development of the scanning system for confocal laser scanning fluorescence microscope

ZHOU Yong-jun; CHEN De-qiang; HUANG Wen-hao; XIA An-dong

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Development of the scanning system for confocal laser scanning fluorescence microscope

更新时间：2020-08-12

- Development of the scanning system for confocal laser scanning fluorescence microscope
- Optics and Precision Engineering Vol. 10, Issue 6, Pages: 582-587(2002)
- 作者机构：
  
  1. 中国科学院化学所, 分子反应动力学实验室北京,100800
  2. 中国科学技术大学, 精密机械及精密仪器系, 安徽, 合肥, 230026
- 作者简介：
- 基金信息：
- DOI：
  CLC： TH742.62
- Received：09 April 2002，
  
  Revised：24 September 2002，
  
  Published Online：15 December 2002，
  
  Published：15 December 2002
- 稿件说明：
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周拥军, 陈德强, 黄文浩, 夏安东. 共聚焦激光扫描荧光显微镜扫描系统研制[J]. 光学精密工程, 2002,(6): 582-587

ZHOU Yong-jun, CHEN De-qiang, HUANG Wen-hao, XIA An-dong,. Development of the scanning system for confocal laser scanning fluorescence microscope[J]. Editorial Office of Optics and Precision Engineering, 2002,(6): 582-587
周拥军, 陈德强, 黄文浩, 夏安东. 共聚焦激光扫描荧光显微镜扫描系统研制[J]. 光学精密工程, 2002,(6): 582-587 DOI：

ZHOU Yong-jun, CHEN De-qiang, HUANG Wen-hao, XIA An-dong,. Development of the scanning system for confocal laser scanning fluorescence microscope[J]. Editorial Office of Optics and Precision Engineering, 2002,(6): 582-587 DOI：

摘要

为适应三维光学微细加工及三维光学信息存储研究的需要

研制了共聚焦激光扫描荧光显微镜的工作台式扫描系统

扫描范围138μm×138μm.工作台采用压电陶瓷驱动器( PZT actuator)驱动的方式来获得高分辨率的位移

采用带柔性铰链的杠杆放大装置来获得较大的位移范围.描述了工作台的工作原理

并对其静态和动态性能进行了测试

实验表明这一扫描系统能很好的应用于共聚焦激光扫描荧光显微镜系统.

Abstract

For adaptation of 3D optical microfabrication and 3D optical data storage

we have developed a stage-scanning system for a confocal laser scanning fluorescence microscope (CLSM) with 138μm x 138μm field of view. The stage employs flexure hinges driven by piezoelectric (PZT) actuators to achieve high positioning accuracy and large scanning area. This paper describes the principle of the stage

and the testing of the static and dynamic properties. The whole system has been testified to work well for confocal laser scanning microscopy.

关键词

Keywords

references

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