On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip

JIN Qing-hui; ZHUANG Gui-sheng; Liu Jing; JIA Chun-ping; WANG Hui-min; ZHAO Jian-long

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On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip

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- On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip
- Optics and Precision Engineering Vol. 13, Issue 2, Pages: 151-157(2005)
- 作者机构：
  
  1. 中国科学院研究生院北京,100039
  2. 中国科学院上海微系统与信息技术研究所上海,200050
  3. 南通大学附属医院,江苏南通,226001
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q657
- Received：14 February 2005，
  
  Revised：09 April 2005，
  
  Published Online：30 April 2005，
  
  Published：30 April 2005
- 稿件说明：
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JIN Qing-hui, ZHUANG Gui-sheng, Liu Jing, et al. On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip[J]. Optics and precision engineering, 2005, 13(2): 151-157.
DOI：

JIN Qing-hui, ZHUANG Gui-sheng, Liu Jing, et al. On-line detection of urinary protein and lactate dehydrogenase isoenzyme by capillary electrophoresis microchip[J]. Optics and precision engineering, 2005, 13(2): 151-157. DOI：

摘要

利用MEMS(微机电系统)标准光刻、湿法腐蚀以及低温键合技术制作了微流控分析芯片。在自制的紫外吸收微芯片生化分析仪上

利用该芯片对临床妊娠高血压和多发性骨髓瘤患者的尿液样本进行了在线芯片电泳分析

得到了与医院常规诊断方法一致的诊断结果

而此法进行一次分析仅需要2 min

且试剂消耗量少

这是常规方法不能比拟的。也以LDH同工酶标准品为分析对象

对芯片在线同工酶诊断进行了初步研究

初步实现了LDH

和LDH

混合样本的片上分离、孵育反应和产物检测。

Abstract

The microfluidic chips were fabricated with standard photolithography

wet etching and low-temperature bonding techniques of MEMS(Micro-electro-mechanical system). Clinical urine samples from gestational hypertension syndrome and multiple myeloma patients were analyzed with the microfluidic chips on home-made UV absorption-based biochem-analysis instrument. The results are in accordance with those from standard urinary proteins assay method. The advantages of microchip-based urinary protein assays such as reduction in analysis time (2 min) and reagent consumption display great superiority over the standard urine assay method. Standard models of lactate dehydrogenase isoenzymes were also selected as the samples for initial study of on-chip LDH assay. The integration of on-chip enzyme separation

enzyme reaction and product detection for mixture of LDH

and LDH

was realized.

关键词

Keywords

references

. HARRISON D J, MANZ A, FAN Z H, et al. Capillary electrophoresis and sample injection systems integrated on a planar glass chip[J]. Anal. Chem., 1992, 64: 1926-1932.

. WOODEY A T, MATHIES R A, Ultra-high-speed DNA sequencing using capillary electrophoresis chips[J]. Anal. Chem., 1995, 67: 3676-3680.

. HOFMAN O, CHE D, CRUICKSHANK K A, et al. Adaptation of capillary isoelectric focusing to microchannels on a glass chip[J]. Anal. Chem., 1999, 71: 678-686.

. HUTT L D, GLAVIN D P, BADA J L, et al. Microfabricated capillary electrophoresis amino acid chirality analyzer for extraterrestrial exploration[J]. Anal. Chem., 1999, 71: 4000-4006.

. 沈同,王镜岩. 生物化学[M]. 北京:高等教育出版社,2002. SHEN T, WANG J Y. Biochemistry[M].Beijing:Higher Education Press, 2002.(in Chinese)

. LEHMANN R, VOELTER W, LIEBICH H M. Capillary electrophoresis in clinical chemistry[J]. J. Chromatogr. B.,1997,697:3-35.

. JENKINS M A, GUERIN M D, Capillary electrophoresis as a clinical tool . J. Chromatogr B.,1996,682:23-24.

. LIU Y J, ROBERT S F, CHRISTOPHER T C,et al. Electrophoretic separation of proteins on microchips[J]. J. Microcolumn Separations, 2000, 12: 407-411.

. CHRISTA L C, SHAKUNTALA D M, HARRISON D J. Microchip-based capillary electrophoresis of human serum proteins[J]. J. Chromatogr. A., 1997, 781: 271-276.

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