Design of micromanipulation instrument for bio-microdissection

doi:null

您当前的位置：

首页 >

文章列表页 >

Design of micromanipulation instrument for bio-microdissection

更新时间：2020-08-12

- Design of micromanipulation instrument for bio-microdissection
- Optics and Precision Engineering Vol. 14, Issue 3, Pages: 416-421(2006)
- 作者机构：
  
  哈尔滨工业大学机器人研究所,黑龙江哈尔滨150001
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q-336
- Received：23 November 2005，
  
  Revised：20 January 2006，
  
  Published Online：30 June 2006，
  
  Published：30 June 2006
- 稿件说明：
移动端阅览
Design of micromanipulation instrument for bio-microdissection[J]. Optics and precision engineering, 2006, 14(3): 416-421.
DOI：

Design of micromanipulation instrument for bio-microdissection[J]. Optics and precision engineering, 2006, 14(3): 416-421. DOI：

摘要

设计了一种能够自动、快速、精确地分离和提取指定细胞群或单个细胞的微操作机器人系统

研制了基于功率超声振动原理的微切割工具(其切割频率在15~50 kHz

振动幅值在0~1 μm可调)和真空吸附式微拾取工具(压力可在0~0.5 MPa精确控制)

应用人体肝脏组织5 μm厚病理石蜡切片进行了显微切割的实验。实验结果证明了超声振动式切割工具对组织无伤害

真空吸附工具收集样品有效且整体系统适应于生物工程中微切割操作的实验需求

具有较高的可靠性、稳定性和准确性。

Abstract

This micromanipulation system provides a technology to procure pure population of targeted cells quickly and accurately for pathology research. Essential micromanipulations were executed by microdissection tool based on ultrasonic vibration (vibration frequency and amplitude at 15~50 kHz and 0~1 μm) and microcollection tool was based on vacuum adsorption (controllable pressure at 0~0.5 MPa). A 5 μm liver embedded sections were dissected and collected successfully in the experiments

the contrast testing experiment indicates that the micromanipulation system is applicable to bio-mcirodissection in high stability and accuracy

in which the ultrasonic vibration is no harm to tissue and vacuum adsorption is in good availability to collection samples.

关键词

Keywords

references

. 闫庆国,黄高升,朱德生,等. 显微切割技术鉴定病毒核内包涵体[J]. 第四军医大学学报,2000,21(1):6-9. YAN Q G., HUANG G S, ZHU D SH, et al. Microdissection technique in the determination of viral intranuclear inclusions[J]. J.Fourth Mil. Med. Univ., 2000,21(1):6-9.(in Chinese)

. BALL H J,MCPARLAND B,DRIUSSI C,et al.Isolating vessels from the mouse brain for gene expression analysisusing laser capture microdissection[J]. Brain Research Protocols,2002,9:206-213.

. SCHUTZE K,LAHR G.Identification of expressed genes by laser mediated manipulation of single cells .Nat. Biotechnology, 1998,16.

. ARAR F,MORISHIMA K.Bio-micro-manipulation .IROS 1997 IEEE,1997,1300-1303.

. 李旭东,宗光华,毕树生,等. 生物工程微操作机器人视觉系统的研究[J]. 北京航空航天大学学报,2002,28(3):249-252. LI X D, ZHONG G H, BI SH SH. Research on global vision system for bioengineering-oriented micromanipulation robot system [J]. Journal of Beijing University of Aeronautics and Astronautics, 2002,28(3):249-252. (in Chinese)

. ARAI F,AMANO T. Microknife using ultrasonic vibration[J].International Symposium On Micromechatronics And Human Science., 2000 IEEE,200,165-2000.

. ARAI F,AMANO T.Mechanical micro-dissection by microknife using ultrasonic vibration and ultra fine touch probe sensor .International Conference on Robotics, 8 Automation, Seoul , 2001 IEEE, May 21-26.

. ZESCH W,BRUNNER M,WEBE A.Vacuum tool for handling micro objects with a nanorobot .Proceedings of the 1997 IEEE,International Conference on Robotics and Automation,Albuquerque,New Mexico,1997,1761-1766.

. EMMERT F F,BUCK M R,CHUQUI R,et al.Immuno. LCM: laser capture microdissection of immunostained frozen sections for RNA analysis[J]. American Journal of Pathology, 1999, 154(1): 61-66.

Views

583

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Design of ultrasonic magnetorheological finishing excitation device for medical bone screw

Development of micro assembly system for friction resistance sensor manufactured based on MEMS

Design and experiment of ultrasonic vibration composite EDM small hole drilling system

Design of a four-arm MEMS microgripper integrated with force sensor

Hybrid-type MEMS microgripper

Related Author

Aiqun XU

Cheng BI

Hongyun WANG

Axiang JI

Liwei XUE

Xiong WANG

Yudie JIA

Hao SHEN

Related Institution

College of Mechanical and Energy Engineering， Zhejiang University of Science and Technology

School of Aeronautical Engineering， Taizhou University

School of Mechanical and Electrical Engineering， Soochow University

Hypervelocity Aerodynamics Institute， China Aerodynamics Research and Development Center

State Key Laboratory of Precision Measuring Technology and Instruments， School of Precision Instrument and Opto-electronics Engineering， Tianjin University

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176855 Email：gxjmgc@ciomp.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰