Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons

QIAO Xiao-yan; LI Gang; HE Bing-jun; LIN Ling

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Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons

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- Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons
- Optics and Precision Engineering Vol. 15, Issue 3, Pages: 337-343(2007)
- 作者机构：
  
  1. 天津大学精密仪器与光电子工程学院
  2. 南开大学生命科学学院天津,300071
  3. 山西大学物理电子工程学院,山西太原,030006
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q682
- Received：16 January 2007，
  
  Revised：19 February 2007，
  
  Published Online：30 June 2007，
  
  Published：30 June 2007
- 稿件说明：
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QIAO Xiao-yan, LI Gang, HE Bing-jun, et al. Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons[J]. Optics and precision engineering, 2007, 15(3): 337-343.
DOI：

QIAO Xiao-yan, LI Gang, HE Bing-jun, et al. Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons[J]. Optics and precision engineering, 2007, 15(3): 337-343. DOI：

摘要

应用全细胞膜片钳技术

研究了急性分离的大鼠海马CA3区锥体神经细胞在波长670 nm、功率5 mW的半导体激光器照射时

其瞬时外向钾通道电流特性。实验发现:弱激光对瞬时外向钾电流

有抑制作用

5 min激光抑制作用达到稳定

去极化至+50 mV时抑制百分比为(40.13±5.19)%(

=10);弱激光对

的抑制作用呈现电压依赖性和可逆性

对照组、照射组和恢复组的最大激活电流密度分别为(398.55±36.49)pA/pF、(238.62±30.78)pA/pF(

n=10

0.01)和(354.08±35.16)pA/pF(

n=10

0.05);激光作用可显著地影响瞬时外向钾通道电流的稳态激活和失活过程

对照组和激光照射组通道的半数激活电压分别为(-27.05±4.53) mV和(-2.10±3.14) mV(

n=10

0.01)

斜率因子分别为(-26.71±6.15) mV和(-20.70±4.38) mV(

n=10

0.05)

半数失活电压分别为(-70.49±7.21) mV和(-81.27±6.26) mV(

n=10

0.01)

斜率因子分别为(9.47±3.54) mV和(9.58±3.02) mV(

n=10

0.05)。结果表明:弱激光作用海马神经元可以改变其瞬时外向钾通道特性

从而影响动作电位的形成和发放

调节神经元的生理功能

有利于受损神经元的恢复和再生。

Abstract

Based on the acutely isolated rat hippocampal CA3 pyramidal neurons irradiated by a semiconductor laser with wavelengh 670 nm and power 5 mW

the current characteristics of transient outward potassium channel were investigated using the whole-cell patch clamp technique. The experiments reveal that low power laser decreases the amplitudes of transient outward potassium current (

) in a voltage-dependent and reversible manner. The percentage of current inhibition is up to (40.13±5.19)% (

=10) depolarizing to +50 mV in laser irradiating 5 min. The maximal activation current densities of control group

irradiation group and recovery group are (398.55±36.49)pA/pF

(238.62±30.78)pA/pF (

n=10

0.01) and (354.08±35.16)pA/pF (

n=10

0.05)

respectively. Laser irradiation markedly affect the activation and inactivation process of

. The half-activation voltage and the slope factor of the steady activation curve change under the laser's exposure. The former alters from (-27.05±4.53) mV to (-2.10±3.14) mV (

n=10

0.01) and the latter from (-26.71±6.15) mV to (-20.70±4.38) mV (

n=10

0.05). The half-inactivation voltage also changes from (-70.49±7.21) mV to (-81.27±6.26) mV (

n=10

0.01) and the slope factor of the inactivation curves from 9.47±3.54 mV to 9.58±3.02 mV (

n=10

0.05). The results indicate that the effect of low power laser on hippocampal neurons could change the current characteristics of transient outward potassium channel of membrane of neurons

and then affect the formation and firing of action potentials. Moreover

physiological function of neurons is adjusted as a result of low power laser irradiation

which might contribute to the restoration and regeneration of injured neurons.

关键词

Keywords

references

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