A novel miniaturized fluorescence detector which using a blue high-power light-emitting diode(LED) as an excitation light source for microfluidic chips analysis was developed and evaluated. LED was driven by the current between source and drain

which was invariable when MOSFET was worked in saturated zone. LED generated stable light with the constant current. Special optical structure was designed and manufactured to focalize the divergent light which was emitted by LED into linear beam of light. The size of the beam of light was about 3.5mm×0.3mm. This linear beam of light could easily calibrate itself to microchannel of microfluidic chips. Then it could reduce complexity of detector and miniaturize the detector. And the bulk of the optical system was about 9.5cm×4cm×17cm. NBD C6-ceramide and high density lipoprotein were used to evaluate performance of the detector. The results indicated that LED excitation light source was stable

the detector had good reproducibility and the detector could be used in the microchips capillary electrophoresis analysis. The LED-induced fluorescence detector was miniaturized.