ZONG Hao-tian,ZHANG Yun-hai,WANG Fa-min,et al.Large field of view line-scanning confocal holographic microscopy[J].Optics and Precision Engineering,2021,29(01):1-9.
Traditional microscopic imaging generally records the intensity information of a sample, but the contrast is poor for translucent or phase tissue. To realize phase tissue non-fluorescence labeling imaging, a line-scanning confocal holographic imaging method was adopted. In line-scanning confocal imaging, a reference light was added to form off-axis image plane digital holography at the confocal slit. The sample was scanned by controlling the movement of the sample. The interference lines obtained were combined to form a two-dimensional hologram, and the distribution of amplitude and phase was obtained by filtering in the frequency domain. The phase striation caused by environmental vibration was rectified using the characteristics of adjacent sections, and large field-of-view holographic imaging was realized by multi-area scanning and splicing. A USAF1951 resolution plate was imaged by line scanning confocal holography, and a jitter correction algorithm was used to reduce the jitter stripes in the reconstructed phase map by 84.7% to obtain three sub-area maps. An imaging field of 1 160 μm × 1 043 μm was achieved by splicing; scanning more sub-regions can help in obtaining a larger field of view and in realizing confocal phase imaging of onion epidermal cells. The experimental results show that the line-scanning confocal holographic imaging method can realize large-field phase imaging of translucent samples and provide guidance and a basis for the development of related instruments.
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