Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section

Shuai HAN; Xinjie ZHANG; Qiao GU; Yao LIU; Xinyi WANG

doi:10.37188/OPE.20223003.0310

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Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section

Micro/Nano Technology and Fine Mechanics | 更新时间：2022-02-17

- Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section
- Optics and Precision Engineering Vol. 30, Issue 3, Pages: 310-319(2022)
- 作者机构：
  
  1.河海大学机电工程学院，江苏常州 213022
  2.苏州大学附属第三医院妇产科，江苏常州 213000
- 作者简介：
- 基金信息：
- DOI：10.37188/OPE.20223003.0310
  CLC： O352
- Received：02 September 2021，
  
  Revised：28 September 2021，
  
  Published：15 February 2022
- 稿件说明：
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韩帅,张鑫杰,顾乔等.非对称截面螺旋流道中微粒的惯性聚焦效应[J].光学精密工程,2022,30(03):310-319.

HAN Shuai,ZHANG Xinjie,GU Qiao,et al.Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section[J].Optics and Precision Engineering,2022,30(03):310-319.
韩帅,张鑫杰,顾乔等.非对称截面螺旋流道中微粒的惯性聚焦效应[J].光学精密工程,2022,30(03):310-319. DOI： 10.37188/OPE.20223003.0310.

HAN Shuai,ZHANG Xinjie,GU Qiao,et al.Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section[J].Optics and Precision Engineering,2022,30(03):310-319. DOI： 10.37188/OPE.20223003.0310.

摘要

为实现生物微粒/细胞的精确操控，提出了一种非对称截面螺旋流道结构的惯性微流控芯片。基于仿真和实验的方法，对不同尺寸微粒在微流道中的惯性聚焦行为进行了研究。设计了一种“L”形截面的螺旋流道，采用仿真软件COMSOL研究微流道中的二次流场及微粒的运动轨迹。使用UV激光切割与等离子清洗键合的工艺制作芯片样件，采用高速摄像机和荧光显微镜分别拍摄6，10和15 μm粒子在微流道中不同流量时的运动轨迹。最后，对粒子运动图片进行堆叠分析，研究微粒的惯性聚焦迁移机理。结果表明：“L”形截面中产生了两对强度不同的非对称二次流场，使得10 μm和15 μm粒子在微流道外圈实现了强聚焦，而6 μm粒子实现了粗聚焦。该研究表明利用非对称二次流可以调节微粒的聚焦位置，为微粒和细胞的精准操控提供新的思路。

Abstract

An inertial microfluidic chip with a spiral microchannel of asymmetric cross-section was proposed to achieve a precise control of biological microparticles/cells. The inertial focusing behavior of particles of different sizes in the microchannel was studied through simulation and experiment. A spiral channel with L shaped cross-section was designed， and the secondary flow field distribution and particle trajectory in the channel were analyzed using COMSOL simulation software. The prototype chip was fabricated by UV laser cutting and plasma cleaning bonding. The trajectories of particles of sizes 6， 10， and 15 μm at different flow rates in the channel were captured by a high speed camera and fluorescent microscope. Finally， the images of particle trajectories were stacked and analyzed and the inertial focusing and migration mechanism of the particles were investigated. The results show that two asymmetric secondary flow vortexes of different strengths are produced in the L shaped cross-section. Furthermore， the particles of 10 and 15 μm sizes focus tightly in the outer ring of the microchannel， whereas those of 6 μm size focus in it roughly. The particle focusing position can be adjusted using asymmetric secondary flow， thus providing new insights into precise particle and cell manipulation.

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references

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