Optimization of living cell delivery parameters in air-assisted atomization

Xintao YAN; Ce WANG; Yao WANG; Feifei SONG; Xiaodong WU

doi:10.37188/OPE.20223014.1725

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Optimization of living cell delivery parameters in air-assisted atomization

Information Sciences | 更新时间：2022-08-08

- Optimization of living cell delivery parameters in air-assisted atomization
- Optics and Precision Engineering Vol. 30, Issue 14, Pages: 1725-1737(2022)
- 作者机构：
  
  1.复旦大学工程与应用技术研究院，上海 200433
  2.中国科学院苏州生物医学工程技术研究所中科院生物医学检验技术重点实验室，江苏苏州 215163
- 作者简介：
- 基金信息：
- DOI：10.37188/OPE.20223014.1725
  CLC： TB96;TP67
- Received：12 October 2021，
  
  Revised：08 December 2021，
  
  Published：25 July 2022
- 稿件说明：
移动端阅览
严心涛,王策,王耀等.空气辅助雾化的细胞递送参数的优化[J].光学精密工程,2022,30(14):1725-1737.

YAN Xintao,WANG Ce,WANG Yao,et al.Optimization of living cell delivery parameters in air-assisted atomization[J].Optics and Precision Engineering,2022,30(14):1725-1737.
严心涛,王策,王耀等.空气辅助雾化的细胞递送参数的优化[J].光学精密工程,2022,30(14):1725-1737. DOI： 10.37188/OPE.20223014.1725.

YAN Xintao,WANG Ce,WANG Yao,et al.Optimization of living cell delivery parameters in air-assisted atomization[J].Optics and Precision Engineering,2022,30(14):1725-1737. DOI： 10.37188/OPE.20223014.1725.

摘要

为实现生物墨水的高效率递送，构建了一种基于空气辅助雾化技术的细胞递送装置，并利用多种方法系统地测量了雾化特性，以及雾化参数对细胞活性的影响。根据粒径的Rosin-Rammler分布规律对雾化均匀度指数进行了定量评估；利用多帧图像叠加以及局部阈值二值化方法对雾化视频进行边界提取，分析雾化角；结合BM3D去噪算法与二值化算法精确定位雾滴位置，实现雾滴运动速度的精确测量。实验结果表明：雾化高度和射流流量均与雾滴粒径正相关；当辅助气体压力高于60 kPa时，会显著影响雾滴均匀度；通过射流流量和雾化高度的参数控制，雾化面积可实现50~1 800 mm

的大宽幅调节；雾滴的运动速度受雾化高度影响较大，且在50 mm雾化高度下运动速度最大，均值可高达14 m/s；雾化高度和辅助空气流量均会显著影响HaCaT细胞活性，在50 mm雾化高度下细胞活化率低至64.01±0.86%（阴性对照组为92.98±3.21%），而在100 mm雾化高度下可高达90.24±0.73%；HaCaT细胞活性在喷涂后72小时内与未喷涂的保持一致。该雾化装置可用于生物墨水的高效率递送，为实现不同面积喷涂或细胞高活化率递送的雾化参数优化设计提供了指导。

Abstract

To achieve high-efficiency delivery of bio-ink， a novel cell delivery device based on air-assisted atomization was developed，and its atomization characteristics and their effect on cell viability were studied. According to the Rosin-Rammler droplet size distribution law， the uniformity index was quantitatively evaluated. Multi-frame image superposition and local threshold binarization were used to extract the boundary of atomization from a video， so that the atomization angle could be analyzed. By combining the BM3D denoising and binarization algorithm to accurately locate the droplet positions， we could calculate the velocity of the droplets. The atomization height and the liquid flow rate are positively correlated to the diameter of the droplets. The pressure of the assistant air has a noticeable effect on the droplet uniformity when this pressure is higher than 60 kPa. By controlling the liquid flow rate and spray height， the spray area can be adjusted to a wide range of 50-1 800 mm

. The velocity of droplets was greatly affected by the spray height. At a spray height of 50 mm， the velocity reached maximum value， with an average value as high as 14 m/s. Both the spray height and the auxiliary air flow rate significantly affect the viability of HaCaT cells. The rate of cell activation was 64.01±0.86% and 90.24±0.73% at a spray height of 50 mm and 100 mm， respectively （the cell activation rate was 92.98±3.21% in the negative control group）. Within 72 hours after spraying， the viability of HaCaT cells was consistent with that of unsprayed cells. The novel atomization device can be used for high-efficiency delivery of bio-ink， and the relevant research results also provide guidance for the optimal design of atomization parameters to achieve spraying of areas of different size and high cell-activation rate delivery.

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