Preparation of genechip microarrays using microfluid digitalization

GENG Xin; HOU Li-ya; YANG Mei; WANG Hong-cheng; ZHANG Wei-yi

doi:10.3788/OPE.20111906.1344

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Preparation of genechip microarrays using microfluid digitalization

Article | 更新时间：2020-08-12

- Preparation of genechip microarrays using microfluid digitalization
- Optics and Precision Engineering Vol. 19, Issue 6, Pages: 1344-1352(2011)
- 作者机构：
  
  南京理工大学机械工程学院,江苏南京,210094
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20111906.1344
  CLC： Q819;TP273
- Received：27 December 2010，
  
  Revised：07 March 2011，
  
  Published Online：25 June 2011，
  
  Published：25 June 2011
- 稿件说明：
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耿鑫, 侯丽雅, 杨眉, 王洪成, 章维一. 微流体数字化技术制备基因芯片微阵列[J]. 光学精密工程, 2011,19(6): 1344-1352

GENG Xin, HOU Li-ya, YANG Mei, WANG Hong-cheng, ZHANG Wei-yi. Preparation of genechip microarrays using microfluid digitalization[J]. Editorial Office of Optics and Precision Engineering, 2011,19(6): 1344-1352
耿鑫, 侯丽雅, 杨眉, 王洪成, 章维一. 微流体数字化技术制备基因芯片微阵列[J]. 光学精密工程, 2011,19(6): 1344-1352 DOI： 10.3788/OPE.20111906.1344.

GENG Xin, HOU Li-ya, YANG Mei, WANG Hong-cheng, ZHANG Wei-yi. Preparation of genechip microarrays using microfluid digitalization[J]. Editorial Office of Optics and Precision Engineering, 2011,19(6): 1344-1352 DOI： 10.3788/OPE.20111906.1344.

摘要

采用以脉冲为微流动基本形态、脉冲当地惯性力为主动力的微流体数字化技术进行了基因芯片微阵列制备实验。在搭建的基于微流体数字化技术的基因芯片微阵列制备系统上

实验验证了脉冲点样系统参量(收敛角2

、微喷嘴内径

、电压幅值

和驱动频率

)对样点直径和脉冲点样稳定性的影响规律。以实验规律为依据

提出了制备样点直径约为100 m的中等密度微阵列的实验路线

制备出了样点平均直径为102.2 m、微阵列密度约为4 000 spot/cm

的基因芯片微阵列(点样溶液为3SSC柠檬酸盐缓冲液)。得到的研究结果可为建立高密度基因芯片脉冲点样技术提供实验研究基础。

Abstract

A preparation experiment of genechip microarrays was carried out by the microfluid digitalization

in which the pulse is basis forms of the microfluidic flows and the microfluidic flows are driven by the pulsed local inertia force in micro channels. Based on the technology

an experimental system to prepare genechip microarrays was built to study the effect the nozzle inside falloff angle 2

nozzle inner diameter

voltage amplitude

and the driving frequency

on the droplet diameter and pulse potting stability. The experimental scheme for preparing the microarrays was proposed and then the moderate microarray with the droplet average diameter of 102.2 m and the density of microarray of 4 000 spot/cm

were manufactured by using 3SSC citrate buffer as spotting solution. The results in this paper can provide a experimental basis for establishing a high-throughput microarray pulse spotting technology.

关键词

Keywords

references

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