ZHOU Li-ping SUN Zhi-jun ZHANG Quan. Auto-focusing and control of micro-vision system[J]. Editorial Office of Optics and Precision Engineering, 2013,21(3): 807-812
ZHOU Li-ping SUN Zhi-jun ZHANG Quan. Auto-focusing and control of micro-vision system[J]. Editorial Office of Optics and Precision Engineering, 2013,21(3): 807-812 DOI: 10.3788/OPE.20132103.0807.
The auto-focusing evaluation function and a controller were researched based on an automated microscopy. At first
the Discrete Wavelet Transform (DWT) and Sobel-Tenengrad function were introduced
and a new auto-focusing evaluation function was proposed by combining the DWT with the Sobel-Tenengrad operator. Then
the defocused and focused sample pictures were used to train the Self-organizing Map (SOM) algorithm in a unsupervised method
and the Particle Swarm Optimization (PSO) was used to accelerate the training process. Finally
an auto-focusing experiment was carried out by using the trained SOM controller. The experimental results show that the new auto-focusing function has the characters of single steep peak and strong robustness to different samples and objective lenses. The results also indicate that the SOM based the controller only take 7.6 steps for auto-focusing process on average
and the focusing speed and stability has been greatly improved compared with that using the mountain climbing method. Moreover
it processes or recognizes the input image only for about 120 ms. The proposed method has met the requirements of auto-focusing of micro-vision system
and obtained good results.
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