Effect of incident laser on resolution of LSCM

XIAO Yun; ZHANG Yun-hai; WANG Zhen; LI Fa-zhi

doi:10.3788/OPE.20142201.0031

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Effect of incident laser on resolution of LSCM

Modern Applied Optics | 更新时间：2020-08-13

- Effect of incident laser on resolution of LSCM
- Optics and Precision Engineering Vol. 22, Issue 1, Pages: 31-38(2014)
- 作者机构：
  
  1. 中国科学院长春光学精密机械与物理研究所,吉林长春,中国,130033
  2. 中国科学院大学北京,中国,100049
  3. 中国科学院苏州生物医学工程技术研究所,江苏苏州,中国,215163
  4. 北京交通大学理学院北京,100044
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20142201.0031
  CLC： TH742.9
- Received：22 November 2012，
  
  Published：15 January 2014
- 稿件说明：
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肖昀, 张运海, 王真等. 入射激光对激光扫描共聚焦显微镜分辨率的影响[J]. 光学精密工程, 2014,22(1): 31-38

XIAO Yun, ZHANG Yun-hai, WANG Zhen etc. Effect of incident laser on resolution of LSCM[J]. Editorial Office of Optics and Precision Engineering, 2014,22(1): 31-38
肖昀, 张运海, 王真等. 入射激光对激光扫描共聚焦显微镜分辨率的影响[J]. 光学精密工程, 2014,22(1): 31-38 DOI： 10.3788/OPE.20142201.0031.

XIAO Yun, ZHANG Yun-hai, WANG Zhen etc. Effect of incident laser on resolution of LSCM[J]. Editorial Office of Optics and Precision Engineering, 2014,22(1): 31-38 DOI： 10.3788/OPE.20142201.0031.

摘要

利用点扩散函数(PSF)研究了入射激光的偏振态和光束的束腰直径对激光扫描共聚焦显微镜(LSCM)分辨率的影响。根据Wolf和Richards的矢量衍射积分

建立了LSCM N-1层界面下的照明PSF模型

对零层和两层界面下的PSF进行了计算分析。结果显示:在零层界面下

圆偏振光入射的PSF在

平面内关于焦点旋转对称

半峰全宽为0.43 μm

向线偏振光入射时PSF在

x、y

方向的半峰全宽分别为0.48

0.39 μm;在圆偏振光入射的PSF中

高斯光束充溢系数为0

5对应的半峰全宽分别为0.43

0.47

0.62

1.49 μm。在两层界面下

当探测深度为50 μm时

圆偏振光PSF的半峰全宽为0.26 μm

向线偏振光入射时PSF在

x、y

方向的半峰全宽分别为0.28

0.24 μm;在圆偏振光入射的PSF中

充溢系数为0

5对应的半峰全宽分别为0.26

0.28

0.32

0.68 μm。以上计算结果表明

和线偏振光相比

圆偏振光在一个方向的分辨率优于线偏振光

在相垂直的另一个方向的分辨率弱于线偏振光

圆偏振光PSF在

平面内关于焦点旋转对称

得到了较好的成像质量;物镜入瞳直径与激光束腰直径比值越小

LSCM的分辨率越好。

Abstract

The effect of the polarization and intensity profile of an incident laser on the resolution of a Laser Scanning Confocal Microscope (LSCM) was investigated.According to the vectorial diffraction integral of Wolf and Richards

a model of the illumination Point Spread Function (PSF) of LSCM with N-1 interfaces was established to calculate and analyze the PSFs on the zero interface and two interfaces.The analysis shows that for the zero interface

the local Full Width at Half Maximum (FWHM) of the PSF with circular polarization is 0.43 μm

which is rotationally symmetric in the

plane

and the FWHMs along

axis and

axis of the PSF with linear polarization are 0.48

0.39 μm

respectively in the

axis.As the filling parameters of a Gaussian light are 0

the FWHMs of the PSF with circular polarization are 0.43

0.47

0.62 μm and 1.49 μm

respectively. For two interfaces

when the probe depth is 50 μm

the FWHM of the PSF with circular polarization is 0.26 μm

and the FWHMs along

axis and

axis of the PSF with linear polarization are 0.28

0.24 μm

respectively in

axis; for the filling parameters of 0

and the probe depth of 50 μm

the FWHMs of the PSF with circular polarization are 0.26

0.28

0.32 and 0.68 μm

respectively. Calculations metioned above show that the resolution of the circularly polarized light is better than that of the linearly polarized light in one direction

but it is worse than that of the linearly polarized light in the perpendicular direction. The PSF with the circularly polarized light is rotationally symmetric in the

plane

therefore

a better image can be obtained. The smaller the filling parameter is

the better the resolution of LSCM is.

关键词

Keywords

references

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