Effect of polarization, phase and amplitude on depletion focus spot in STED

WEI Tong-da; ZHANG Yun-hai; TANG Yu-guo

doi:10.3788/OPE.20142205.1157

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Effect of polarization, phase and amplitude on depletion focus spot in STED

更新时间：2020-08-13

- Effect of polarization, phase and amplitude on depletion focus spot in STED
- Optics and Precision Engineering Vol. 22, Issue 5, Pages: 1157-1164(2014)
- 作者机构：
  
  1. 中国科学院长春光学精密机械与物理研究所,吉林长春,中国,130033
  2. 中国科学院大学北京,中国,100049
  3. 中国科学院苏州生物医学工程技术研究所,江苏苏州,中国,215163
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20142205.1157
  CLC： TH742.9
- Received：21 March 2013，
  
  Revised：11 April 2013，
  
  Published：25 May 2014
- 稿件说明：
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魏通达, 张运海, 唐玉国. 偏振态、相位和振幅对受激辐射损耗中损耗光焦斑的影响[J]. 光学精密工程, 2014,22(5): 1157-1164

WEI Tong-da, ZHANG Yun-hai, TANG Yu-guo. Effect of polarization, phase and amplitude on depletion focus spot in STED[J]. Editorial Office of Optics and Precision Engineering, 2014,22(5): 1157-1164
魏通达, 张运海, 唐玉国. 偏振态、相位和振幅对受激辐射损耗中损耗光焦斑的影响[J]. 光学精密工程, 2014,22(5): 1157-1164 DOI： 10.3788/OPE.20142205.1157.

WEI Tong-da, ZHANG Yun-hai, TANG Yu-guo. Effect of polarization, phase and amplitude on depletion focus spot in STED[J]. Editorial Office of Optics and Precision Engineering, 2014,22(5): 1157-1164 DOI： 10.3788/OPE.20142205.1157.

摘要

针对受激辐射损耗（STED）超分辨显微术分辨率不够高的问题，研究了损耗光偏振态、相位和振幅多种物理量对焦斑的影响，以形成半峰全宽窄的圆环形损耗光焦斑。根据Richards-Wolf矢量衍射理论，建立了偏振态、相位和偏振态作用下的损耗光焦斑模型；计算了不同偏振态、不同相位振幅调制参数下损耗光焦斑的分布情况；通过优化各参量得到有效激发荧光的分布。计算结果表明，应用切向偏振时的损耗光焦斑半峰全宽优于应用径向偏振和圆偏振；相位和振幅的调制作用均能减小半峰全宽；优化后有效激发荧光的理论半峰全宽仅为13.2nm。采用损耗光的偏振态、相位和振幅对损耗光焦斑进行整形，能够有效减小半峰全宽，获得较高的理论分辨率，比仅使用单一物理量的效果更好；应用切向偏振光能够获得高质量的损耗光焦斑和超衍射极限的分辨能力，根据不同实际情况选择相位或振幅调制的方法可进一步提高分辨率。

Abstract

As Stimulated Emission Depletion (STED) super-resolution microscopy has a lower resolution

this paper explores the effects of multiphysical variables including light polarization

phases and amplitudes on the focal spots

in order to form a ring-shape depletion focus spot with sharper Full Width at Half Maximum(FWHM) and to increase the resolution of the microscopy.According to Richards-Wolf vector theory

the models of depletion focus spots were established under the actions of polarization

phase and amplitude

the focus spot distribution was calculated in different polarization

phase and amplitude conditions and the distribution of effective fluorescent excitation was obtained by optimizing the parameters.The results show that: by using azimuthal polarization light as depletion

the FWHM is better than those of radial or circular ones; modulations of phase and amplitude are able to reduce the FWHM

and the theoretical value of effective fluorescent excitation FWHM is only 13.2 nm after optimization.By using polarization state

phase and amplitude to modulate the depletion focus spot

the FWHM can be reduced and a higher resolution can be obtained

which is more effective than that using only single physical variable.Moreover

the high quality depletion focus spot and super-resolution ability can be obtained with azimuthal polarization light in STED and the resolution can further be optimized by phase or amplitude modulations based on different realities.

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references

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