Development of static microscopic spectral imaging system

QI Min-jun; WANG Xin-quan; YU Cui-rong; PAN Dong-ning; XIA Wei-wei

doi:10.3788/OPE.20152305.1240

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Development of static microscopic spectral imaging system

更新时间：2020-08-13

- Development of static microscopic spectral imaging system
- Optics and Precision Engineering Vol. 23, Issue 5, Pages: 1240-1245(2015)
- 作者机构：
  
  青岛市光电工程技术研究院,山东青岛,266109
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20152305.1240
  CLC： TH744.1;TH742.9
- Received：07 January 2015，
  
  Revised：12 February 2015，
  
  Published：25 May 2015
- 稿件说明：
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齐敏珺, 王新全, 于翠荣等. 静态显微光谱成像系统的研制[J]. 光学精密工程, 2015,23(5): 1240-1245

QI Min-jun, WANG Xin-quan, YU Cui-rong etc. Development of static microscopic spectral imaging system[J]. Editorial Office of Optics and Precision Engineering, 2015,23(5): 1240-1245
齐敏珺, 王新全, 于翠荣等. 静态显微光谱成像系统的研制[J]. 光学精密工程, 2015,23(5): 1240-1245 DOI： 10.3788/OPE.20152305.1240.

QI Min-jun, WANG Xin-quan, YU Cui-rong etc. Development of static microscopic spectral imaging system[J]. Editorial Office of Optics and Precision Engineering, 2015,23(5): 1240-1245 DOI： 10.3788/OPE.20152305.1240.

摘要

提出一种基于液晶可调谐滤光片(LCTF)的显微光谱成像系统。介绍了系统的结构原理和实现方式

完成了原理样机的研制

并给出了实验结果。基于模块化的设计思想

通过标准C转接口与显微镜视频接口连接实现了显微光谱成像。采用LCTF作为分光元件

实现了较宽波长范围内通过电调谐方式来控制透过波长

系统具有无运动部件、调谐速度快且可通过编程灵活选择波长的优点。采用完全对称的中继光学结构

将LCTF置于准直光路中消除可能由LCTF引起的像差;开发了光谱图像采集与分析软件

实现了光谱图像采集的有序控制和数据分析。研制了原理样机

并对头发纤维和南瓜茎纵切片进行了显微光谱成像实验。实验在400~720 nm每隔10 nm采集一幅图像组成数据立方体

通过光谱分析实现了对头发纤维不同来源的区分以及对南瓜茎不同染色部位的提取。实验结果表明:提出的系统能够进行显微光谱成像

获得的数据质量良好

扩展了传统显微成像技术的功能和应用。

Abstract

A microscopic spectral imaging system was proposed based on a Liquid Crystal Tunable Filter (LCTF). The systemic structure principle and operating method were introduced and a prototype was developed. Using the idea of modular design

a spectral imaging module was developed to cooperate with a microscope by connecting a standard C switch interface and a microscopic video interface. The LCTF was used as light filter in the spectral imaging module to select the light of certain wavelength by an electrical tuning mode. Without moving parts in the system

it could offer a faster tuning speed and could select wavelength by programming in flexibility. Then

the LCTF was placed in the collimated path to eliminate the possible aberrations. Furthermore

the software for image collection and analysis was developed to perform the order control and data analysis for the spectral images. The prototype was completed and microscopic spectral imaging experiment was performed for hair fibers and longitudinal sections of pumpkin stem. The spectral images with wavelength varied from 400 nm to 720 nm in 10 nm intervals were obtained. The results show that different origins of hair fibers have been distinguished and also different parts of longitudinal sections of pumpkin stem are extracted. The proposed system completes microscopic spectral imaging and collects good quality data

by which the function and application areas of the traditional microscope have been extended.

关键词

Keywords

references

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