Effects of low level laser irradiation on cell cycle and intracellular calcium concentration of osteoblastic cells under mechanical strain

SHI Rui-xin; ZHOU Yan-min; HU Min; CHEN Yuan-ping; ZHUANG Jin-liang

doi:10.3788/OPE.20152307.1984

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Effects of low level laser irradiation on cell cycle and intracellular calcium concentration of osteoblastic cells under mechanical strain

更新时间：2020-08-12

- Effects of low level laser irradiation on cell cycle and intracellular calcium concentration of osteoblastic cells under mechanical strain
- Optics and Precision Engineering Vol. 23, Issue 7, Pages: 1984-1989(2015)
- 作者机构：
  
  1. 吉林大学口腔医院正畸科, 吉林长春 130021
  2. 吉林大学口腔医院种植科,吉林长春,130021
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20152307.1984
  CLC： R783.5;R318.51
- Received：09 May 2015，
  
  Revised：09 June 2015，
  
  Published：25 July 2015
- 稿件说明：
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史瑞新, 周延民, 胡敏等. 低能量激光照射对受张力的成骨细胞周期和胞内Ca<sup>2+</sup>浓度的影响[J]. 光学精密工程, 2015,23(7): 1984-1989

SHI Rui-xin, ZHOU Yan-min, HU Min etc. Effects of low level laser irradiation on cell cycle and intracellular calcium concentration of osteoblastic cells under mechanical strain[J]. Editorial Office of Optics and Precision Engineering, 2015,23(7): 1984-1989
史瑞新, 周延民, 胡敏等. 低能量激光照射对受张力的成骨细胞周期和胞内Ca<sup>2+</sup>浓度的影响[J]. 光学精密工程, 2015,23(7): 1984-1989 DOI： 10.3788/OPE.20152307.1984.

SHI Rui-xin, ZHOU Yan-min, HU Min etc. Effects of low level laser irradiation on cell cycle and intracellular calcium concentration of osteoblastic cells under mechanical strain[J]. Editorial Office of Optics and Precision Engineering, 2015,23(7): 1984-1989 DOI： 10.3788/OPE.20152307.1984.

摘要

对成骨样细胞施加了周期性张力

探讨了成骨细胞MG-63受张力时的细胞周期和胞内Ca

浓度的变化规律以及低能量激光照射(LLLI)对此变化规律的影响

揭示了LLLI促进骨形成的机制。实验首先将MG-63细胞随机分成对照组、加力组、激光加力组3组

用流式细胞术检测各组细胞周期。然后

将MG-63细胞分为张力组和激光张力组两组

对胞内Ca

浓度变化进行测定

对2组细胞分别加力0

60 min

再对激光张力组施加激光照射1 min后收取细胞

并用荧光探针Fluo-3-AM测定成骨样细胞内Ca

浓度和Ca

阳性细胞百分比。结果显示:MG-63细胞加载张力后

细胞增殖指数提高

施加LLLI后受力的成骨细胞增殖指数进一步提高。张力引起胞内Ca

浓度增加

变化剧烈

LLLI使变化曲线平缓

且胞内Ca

浓度和Ca

阳性细胞百分比增加。实验结果表明:LLLI可促进受张力的成骨细胞增殖

推测可能是通过调节成骨细胞内Ca

浓度的变化规律和水平来完成的。

Abstract

The cycle strain was exerted on osteoblastic cells

and the changes of cell cycle and intracellular calcium concentration of osteoblastic MG-63 cells under a mechanical stretching were explored and the effect of Low Level Laser Irradiation (LLLI) on the changed rule was revealed. Firstly

MG-63 cells were divided into 3 groups: control group

strain group and LLLI-strain group

and the cell cycles of these MG-63 cells were measured by a flow cytometry (FCM). Then

the MG-63 cells were divided into 2 groups: strain group and LLLI-strain group

and their intracellular calcium concentration was measured by the FCM and a fluorescent indicator fluo-3/AM at 0

30 and 60 min under the stretching. However

the LLLI-strain group would receive LLLI for 1 min after the stretching. Experimental results indicate that strain groups present higher proliferation indexes as compared to the control group. The laser-strain groups present higher proliferation index than other two groups. For laser-strain group

the concentration of the intracellular calcium ion increases gently

but that in strain group has increased drastically. It concludes that the LLLI promotes the proliferation of MG-63 cells under the stretching

and it may be achieved by regulating the changed rhythm of concentration of the intracellular Ca

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references

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史瑞新,周延民,陈远萍.低能量激光照射对受张力刺激的成骨细胞周期和细胞凋亡的影响[J]. 中国老年学杂志, 2011,31(5): 839-841. SHI R X,ZHOU Y M,CHEN Y P. Effects of low-level laser irradiation on cell cycle and apoptosis of osteoblastic cells under mechanical strain [J]. Chinese Journal of Gerontology, 2011,31(5): 839-841. (in Chinese)

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