MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker. Conventional methods for the detection of miRNA are time-consuming and complex. Herein
a portable rapid miRNA detection system was designed. This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique
and analyzes the fluorescence data to obtain the changes in miRNA. Through our experiments
we observed that for reagent concentrations in the range 0.1-1
μ
mol
the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5%
and the repeatability was ≥ 95%. Our roll amplification experiment involving the coronary heart disease diagnostic marker
miR-499
confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.
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references
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