Design of board spectrum and long work distance microscope objective for weak fluorescence signal detection

Xiao-hui YU; Qun YUAN

doi:10.3788/OPE.20182607.1588

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Design of board spectrum and long work distance microscope objective for weak fluorescence signal detection

Modern Applied Optics | 更新时间：2020-08-13

- Design of board spectrum and long work distance microscope objective for weak fluorescence signal detection
- Optics and Precision Engineering Vol. 26, Issue 7, Pages: 1588-1595(2018)
- 作者机构：
  
  南京理工大学电子工程与光电技术学院, 江苏南京 210094
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20182607.1588
  CLC： O439;TH742
- Received：06 December 2017，
  
  Accepted：09 January 2018，
  
  Published：25 July 2018
- 稿件说明：
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Xiao-hui YU, Qun YUAN. Design of board spectrum and long work distance microscope objective for weak fluorescence signal detection[J]. Optics and precision engineering, 2018, 26(7): 1588-1595.
DOI：

Xiao-hui YU, Qun YUAN. Design of board spectrum and long work distance microscope objective for weak fluorescence signal detection[J]. Optics and precision engineering, 2018, 26(7): 1588-1595. DOI： 10.3788/OPE.20182607.1588.

摘要

针对癌细胞突变基因诱导荧光信号弱、光谱覆盖范围宽、现有显微镜不能检测等局限，本文设计了光谱波段为450~800 nm、数值孔径为0.95的荧光显微物镜。物镜采用++-结构，因宽光谱、大数值孔径像差校正难度大、透镜片数多、装调困难，前组设置成敏感组分，承载物镜装调的调校功能，承担90%以上光焦度；中间组为弱光焦度组分，用于校正大数值孔径下的二级光谱，显著降低了二级光谱校正元件的加工难度；后组为负光焦度组分，用于平像场和增大物镜的工作距离。物镜的设计参数为：总长58 mm、工作距0.21 mm、视场0.625 mm、倍率40×、数值孔径0.95，结果表明：其像质接近衍射极限，畸变小于0.2%，满足多种癌细胞突变基因的弱信号生物监测设计要求。

Abstract

Because the fluorescence signal induced by a cancer cell is weak and has a wide spectral range

along with the detection limits of existing microscopes

this paper proposes fluorescence microscope objectives with a spectral range of 450-800 nm and a numerical aperture of 0.95. The objective used "++-" structure because the correction of aberration

caused by a wide spectrum and high numerical aperture

is difficult. The objective must include a set number of lenses and the alignment process is difficult; the front group

considering more than 90% focal power

is set as the sensitive component

which is useful for adjusting the alignment process; the middle group is the weak focal component

which is used to correct the secondary spectrum caused by the large numerical aperture

so that the difficulty in manufacturing the apochromatic elements is reduced significantly. The posterior group is the negative component

which is useful for flattening the image field and increasing the working distance of the objective. The objective parameters are as follow:the total length is 58 mm

the working distance is 0.21 mm

the magnification is 40×

and the full field is 0.625 mm. The results show that the image quality nearly reaches the diffraction limit and the distortion is less than 0.2%

meeting the surveilling requirements of the weak fluorescence signal from cancer cells.

关键词

Keywords

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