Design and fabrication of three-dimensional dynamic cell culture micro-devices

Chong LIU; Tao LIU; Juan WEI; Yang JIANG; Xue-cui MEI; Jing-min LI

doi:10.3788/OPE.20182607.1672

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Design and fabrication of three-dimensional dynamic cell culture micro-devices

Micro/Nano Technology and Fine Mechanics | 更新时间：2020-08-13

- Design and fabrication of three-dimensional dynamic cell culture micro-devices
- Optics and Precision Engineering Vol. 26, Issue 7, Pages: 1672-1679(2018)
- 作者机构：
  
  1.大连理工大学辽宁省微纳米及系统重点实验室, 辽宁大连 116024
  2.厦门大学机电工程系, 福建厦门 361005
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20182607.1672
  CLC： TB559;TN405
- Received：03 January 2018，
  
  Accepted：15 February 2018，
  
  Published：25 July 2018
- 稿件说明：
移动端阅览
Chong LIU, Tao LIU, Juan WEI, et al. Design and fabrication of three-dimensional dynamic cell culture micro-devices[J]. Optics and precision engineering, 2018, 26(7): 1672-1679.
DOI：

Chong LIU, Tao LIU, Juan WEI, et al. Design and fabrication of three-dimensional dynamic cell culture micro-devices[J]. Optics and precision engineering, 2018, 26(7): 1672-1679. DOI： 10.3788/OPE.20182607.1672.

摘要

细胞培养是进行细胞研究的基础，为了在细胞体外培养时提供一种近似于体内的微环境，设计了一种可供细胞三维动态培养的微器件。首先设计了用于输运流体的微通道网络，培养池对称布置于微通道网络中，通过一系列"多进多出"型微通道分别与进样口和出样口相连。利用Comsol软件中的层流物理场和多孔介质物理场耦合对培养池内的流场进行仿真，通过比较流场的均一性和稳定性优化微通道网络结构。然后，采用静电直写技术在培养池内集成聚己内酯（PCL）三维支架，构建细胞三维培养空间。最后，封合微器件，检测微器件培养池内的流体流动情况，并进行细胞实验。实验结果表明，"2×2"型微器件培养池内的流体稳定性和均一性较好；PCL三维支架的纤维间距400

m，纤维直径80

m，孔隙率64%，细胞存活率达到90%以上。该细胞三维动态培养微器件更好地模拟了生物体内细胞生存所需的微环境，培养池内的细胞生长良好，满足设计要求。

Abstract

Cell culture is the basis of cell research. In order to provide an approximate microenvironment for cell culture

a micro-device for three-dimensional and dynamic cell culture was designed. First

a micro-channel network was designed for transportation of liquid. The cell culture chamber was symmetrically arranged in the micro-channel network. A series of "multiple input and multiple output" micro-channels were connected with the inlet and outlet of the cell culture chamber. Second

the physical fields of laminar and porous media in the COMSOL software were coupled

which was used to simulate the velocity of the liquid in the cell culture chamber of the micro-device. The network structure of micro-channels was optimized by comparing the homogeneity and stability of the flow field. Third

the electrohydrodynamic direct-writing technology was used to integrate polycaprolactone (PCL) three-dimensional scaffolds in the cell culture chamber

which constructed the three-dimensional culture space. Finally

after the welding of the micro-device

the fluid flow condition in the cell culture chamber of the micro-device was tested

and cell experiments were performed. The results show that the fluid stability and homogeneity in the cell culture chamber of a "2×2" micro-device are appropriate. The fiber spacing of the three-dimensional scaffolds is 400

the diameter is 80

and the porosity is 64%. Besides

the cell survival rate is more than 90%. The micro-device

which can be used for three-dimensional dynamic cell culture

precisely simulates the microenvironment needed for the survival of cells in vivo. Cells in the culturing chamber grew well; therefore

the micro-device can satisfy the design requirements.

关键词

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