High-throughput digital capillary microarray

Ya-jun QIU; Jin-ze LI; Chuan-yu LI; Zhi-qi ZHANG

doi:10.3788/OPE.20192706.1237

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High-throughput digital capillary microarray

Life Science Instrument | 更新时间：2020-08-13

- High-throughput digital capillary microarray
- Optics and Precision Engineering Vol. 27, Issue 6, Pages: 1237-1244(2019)
- 作者机构：
  
  1.中国科学院苏州生物医`学工程技术研究所中国科学院生物医学检验技术重点实验室，江苏苏州 215163
  2.中国科学院大学生命科学学院，北京 100049
  3.中国科学技术大学，安徽合肥 230026
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20192706.1237
  CLC： TP212.3
- Received：05 December 2018，
  
  Accepted：02 January 2019，
  
  Published：15 June 2019
- 稿件说明：
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Ya-jun QIU, Jin-ze LI, Chuan-yu LI, et al. High-throughput digital capillary microarray[J]. Optics and precision engineering, 2019, 27(6): 1237-1244.
DOI：

Ya-jun QIU, Jin-ze LI, Chuan-yu LI, et al. High-throughput digital capillary microarray[J]. Optics and precision engineering, 2019, 27(6): 1237-1244. DOI： 10.3788/OPE.20192706.1237.

摘要

针对数字PCR体系样品的分割方式，开发了一款数字PCR体系样品分割芯片，用于微量生物样品检测。利用微机电系统(MEMS)制备阵列化的硅基片，采用硅片高效低损伤超精密磨削减薄工艺对硅基片进行减薄，结合化学改性方法，成功制备了表面疏水孔壁亲水的毛细管微阵列芯片。通过扫描电子显微镜(SEM)对芯片结构进行表征，SEM结果显示，芯片结构为通孔微阵列。通过接触角表征芯片表面的疏水性，对比化学处理前后芯片表面的接触角，结果表明化学处理后芯片表面疏水，接触角为118°。通过能谱(EDS)表征芯片孔壁的亲水性，结果表明，芯片孔壁只有Si，O两种元素，形成亲水基团，因此，芯片孔壁亲水。通过测量显微镜和荧光显微镜表征芯片的样品分割性能，结果表明芯片将样品分割为均一的独立单元。通过激光共聚焦扫描仪表征，直观地反应了芯片的整体样品分割效果，通过计算芯片的样品填孔率为93.8%。本文成功制备了表面疏水孔壁亲水的毛细管微阵列芯片，该芯片具有优异的样品分割性能，在生物医学领域具有广阔的应用前景。

Abstract

A digital Polymerase Chain Reaction (PCR) system sample segmentation chip was developed for the "division" method for samples of a digital PCR system

and a capillary microarray was used for trace biological sample detection. First

arrayed silicon substrates were fabricated using Micro-electron-mechanical Systems(MEMS) technology

subsequently silicon wafers were thinned by the process of high-efficiency

low-damage

and ultra-precision grinding and then combined through chemical modification methods. A capillary microarray with a hydrophobic surface and hydrophilic inner wall was successfully prepared. The structure of the capillary microarray was characterized by scanning electron microscopy (SEM). SEM results showed that the structure of the capillary microarray is a through-hole microarray. The hydrophobicity of the surface of the capillary microarray was characterized by the contact angle

and the contact angles of the surface before and after chemical treatment were compared. The results show that the surface of the capillary microarray is hydrophobic after chemical treatment

and the contact angle is 118°. The hydrophilicity of the inner walls of the capillary microarray was characterized by an energy dispersive spectrometer (EDS). The results show that only Si and O elements are present in the inner walls of the capillary microarray

forming a hydrophilic group (—Si—OH). Thus

the inner walls of the capillary microarray were hydrophilic. The sample segmentation performance of the capillary microarray was characterized by measurement microscopy and fluorescence microscopy. The results show that the capillary microarray divided the sample into uniform independent units. Characterized by the laser confocal scanner

the overall sample segmentation effect of the capillary microarray is intuitively reflected

and the sample addition rate of the chip is 93.8% by counting and calculation. Finally

a capillary microarray chip with a hydrophobic surface and hydrophilic inner wall was successfully prepared and exhibited excellent sample segmentation performance

which offers broad application prospects for the field of biomedics.

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