Design and fabrication of device for cell dynamic culture in microfluidic chip

Yang JIANG; Juan WEI; Shu-qing YIN; Lai-qian DING; Jing-min LI

doi:10.3788/OPE.20192709.2020

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Design and fabrication of device for cell dynamic culture in microfluidic chip

更新时间：2020-08-13

- Design and fabrication of device for cell dynamic culture in microfluidic chip
- Optics and Precision Engineering Vol. 27, Issue 9, Pages: 2020-2027(2019)
- 作者机构：
  
  大连理工大学辽宁省微纳米及系统重点实验室，辽宁大连 116024
- 作者简介：
  
  LI Jing-min, E-mail: jingminl@dlut.edu.cn
- 基金信息：
- DOI：10.3788/OPE.20192709.2020
  CLC： TB559; TN405
- Received：28 January 2019，
  
  Accepted：16 March 2019，
  
  Published：15 September 2019
- 稿件说明：
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Yang JIANG, Juan WEI, Shu-qing YIN, et al. Design and fabrication of device for cell dynamic culture in microfluidic chip[J]. Optics and precision engineering, 2019, 27(9): 2020-2027.
DOI：

Yang JIANG, Juan WEI, Shu-qing YIN, et al. Design and fabrication of device for cell dynamic culture in microfluidic chip[J]. Optics and precision engineering, 2019, 27(9): 2020-2027. DOI： 10.3788/OPE.20192709.2020.

摘要

基于微流控芯片的细胞培养能有效模拟细胞在体生存微环境，为实现微流控芯片内细胞的长期动态培养及实时观察，设计并制作了一种微流控芯片细胞动态培养装置。首先

采用分体式设计，利用SolidWorks分别构建培养箱、控制箱和载泵箱的三维模型，根据性能要求设计控制系统并配备硬件。接着，利用Comsol软件中的焦耳热模块对加热器ITO玻璃进行热力学仿真，通过分析温度场验证可行性。然后，搭建培养装置，进行调试，并检验其稳定性。最后，进行细胞培养实验。采用分体式设计可有效减小培养箱体积，实现与显微观测系统的兼容；培养装置对温湿度的控制稳定性良好；细胞生长状态良好，生长曲线呈“S”形，细胞存活率达到95%以上。该分体式微流控细胞动态培养装置可长期为细胞培养提供所需环境，并可进行实时观察，满足设计要求。

Abstract

Cell culture is the basis of cell research. In order to realize long-term dynamic culture and real-time observation of cells in a microfluidic chip

a microfluidic cell dynamic culture device was designed and fabricated. First

based on the "split" arrangement idea

a three-dimension model of the culture box

control box

and pump box were set up using the software

SolidWorks; hardware and software were designed taking the functional demands into consideration. Second

based on the Comsol software platform

the method of Joule heating was used to analyze the thermodynamic characteristics of the device's heater

namely

ITO glass; the temperature distribution was then obtained from the simulation results. Third

the entire device model was established and its stability was tested. Finally

cell culture experiments were performed. The split design can effectively reduce the volume of the incubator and achieve compatibility between the incubator and the microscopic observation system. In addition

the temperature and humidity of the device are stable

cell growth is in the "S" trend

and the cell survival rate is more than 95%. The split-body cell dynamic culture device offers a satisfactory environment condition for cell culture

in which cells can be observed in real time. As such

the device can satisfy relevant design requirements.

关键词

Keywords

references

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