Cell imaging by holographic lens-free microscopy

Xue WANG; Hong-yao LIU; Xin-chao LU; Xu-qing SUN; Yi-fei YE; Cheng-jun HUANG

doi:10.3788/OPE.20202808.1644

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Cell imaging by holographic lens-free microscopy

Modern Applied Optics | 更新时间：2020-08-14

- Cell imaging by holographic lens-free microscopy
- Optics and Precision Engineering Vol. 28, Issue 8, Pages: 1644-1650(2020)
- 作者机构：
  
  1.中国科学院微电子研究所, 北京 100029
  2.中国科学院大学, 北京 100049
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20202808.1644
  CLC： O436.1;Q336
- Received：09 January 2020，
  
  Revised：26 February 2020，
  
  Accepted：26 February 2020，
  
  Published：15 August 2020
- 稿件说明：
移动端阅览
Xue WANG, Hong-yao LIU, Xin-chao LU, et al. Cell imaging by holographic lens-free microscopy[J]. Optics and precision engineering, 2020, 28(8): 1644-1650.
DOI：

Xue WANG, Hong-yao LIU, Xin-chao LU, et al. Cell imaging by holographic lens-free microscopy[J]. Optics and precision engineering, 2020, 28(8): 1644-1650. DOI： 10.3788/OPE.20202808.1644.

摘要

为了同时满足较大的视场和较高分辨率的需求，开发了一套全息无透镜显微成像系统和配套算法，实现对微米级样品的无透镜显微成像。搭建了一套由LED光源、针孔、被测样品与CMOS图像传感器组成的全息无透镜显微成像系统，并对针孔直径、成像面尺寸、光源到样品的距离，以及样品面到CMOS图像传感器的距离进行了优化。其次，开发了从系统采集的全息图中恢复样品图像的角谱法算法。最后，使用该成像系统和配套算法，分别对具有微米级结构分辨率测试靶，和肺癌细胞悬浮液进行了显微成像。该全息无透镜显微成像系统的分辨率为4.4

m，成像视场尺寸为5.7 mm×4.3 mm，实现了微米级结构和肺癌细胞较清晰的显微成像。全息无透镜显微成像系统结构简单、无像差干扰，可以实现大视场下较高分辨率的显微成像。

Abstract

This study develops a lens-free microscope system and an algorithm to realize microscopic imaging with a relatively large field of view and high resolution. The proposed system consists of an LED light source

a pinhole

a sample holder

and a CMOS imager. The system parameters

such as the pinhole diameter

size of the imager

distance between the LED and sample

and distance between the sample and imager were studied and optimized. Furthermore

an angular spectrum method was developed for recovering sample images from holograms captured by the CMOS imager.Finally

the developed system was employed to demonstrate lens-free microscopic imaging of a micropatterned testing chip with a standard resolution in addition to that of lung cancer cells in suspension.The developed holographic lens-free microscopic system has a resolution of 4.4

m and an imaging field of view up to 5.7 mm×4.3 mm; furthermore

it is capable of imaging the micropatterns in the test chip as well as individual lung cancer cells.Thus

the proposed holographic lens-free microscopic system demonstrated microscopic imaging with a relatively large field of view and high resolution; moreover

it exhibited the advantages of a simple structure and the avoidance of aberration interference.

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references

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