Experimental study on grinding technology of microchannel for self-driven detection chip

Jin XIE; Ao-dian GUO; Kuo LU; Min-jian LUO; Hong-jie SHEN

doi:10.3788/OPE.20202808.1743

您当前的位置：

首页 >

文章列表页 >

Experimental study on grinding technology of microchannel for self-driven detection chip

Micro/Nano Technology and Fine Mechanics | 更新时间：2020-08-14

- Experimental study on grinding technology of microchannel for self-driven detection chip
- Optics and Precision Engineering Vol. 28, Issue 8, Pages: 1743-1750(2020)
- 作者机构：
  
  华南理工大学机械与汽车工程学院, 广东广州 510640;广州迪澳生物科技有限公司, 广东广州 510663
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20202808.1743
  CLC： TH16;O65
- Received：06 January 2020，
  
  Revised：13 March 2020，
  
  Accepted：13 March 2020，
  
  Published：25 August 2020
- 稿件说明：
移动端阅览
Jin XIE, Ao-dian GUO, Kuo LU, et al. Experimental study on grinding technology of microchannel for self-driven detection chip[J]. Optics and precision engineering, 2020, 28(8): 1743-1750.
DOI：

Jin XIE, Ao-dian GUO, Kuo LU, et al. Experimental study on grinding technology of microchannel for self-driven detection chip[J]. Optics and precision engineering, 2020, 28(8): 1743-1750. DOI： 10.3788/OPE.20202808.1743.

摘要

针对病原体检测用芯片需要蠕动泵和离心机外加驱动的问题，设计微V槽流道的自驱动芯片，研究微液体流动的微流道拓扑结构及其精密磨削技术。因为激光等物理加工难以保证微拓扑结构精度，所以采用金刚石磨削技术实现石英玻璃表面的微V槽流道精密加工。基于多轴联动技术和机械物理去除原理开发了砂轮微尖端的高效精密在位修整工艺，可将磨粒精密修尖至同一角度，进行机械精密复制的塑性域微磨削。然后，实验分析微V槽流道的尖角、表面粗糙度、梯度等对微液体流速的影响。最后，制造出病原体检测的微流控芯片。研究结果显示，更大梯度、更小尖角和更小粗糙度以及尖角端分布的纳米流道可以大幅提高微液体流速。而且，微流道的V槽尖端半径为15

m，表面粗糙度为30 nm，可诱导微液体运动。在此基础上，研发的自驱动微流控芯片不需离心机就能够检测出布鲁氏菌的病原体核酸，检测灵敏度可以小于100 ag/

L。

Abstract

Pathogen detectionrequires additional driving witha peristaltic pump and centrifuge. Hence

a self-driven microfluidic chip was designed with micro-V-groove channels

and its topologic structure and precision grinding were studied in relation to flow.Because it is difficult for physical processing

such as laser processing

to ensure topologic microform accuracy

diamond grinding was employed to machine the micro-V-groove channelsprecisely on a quartz glass surface. The key was to develop efficient and precision on-machine truing of a wheel-V-tip with the same grain-tip angle through multiaxis control and mechanical physical removal and subsequently to perform ductile-modemicrogrinding with mechanical precision copy. Furthermore

the influence of themicro-V-groove angle

roughness

gradient

etc. on microliquid flowing velocity were experimentally investigated. Finally

a microfluidic chip was manufactured for pathogen detection. It was found that larger gradient

smaller angle

finer surface roughness

and at-V-tip distributed nanochannels lead to a much larger flow velocity in the microfluidic chip. Accordingly

the micro-V-grooves can be ground to attain a surface roughness of 30 nm and tip radius of 15

which induces microliquid flow. As a result

the developed self-driven microfluidic chip can detect Brucella pathogen nucleic acids with a detection accuracy of 100 ag/

Lor lesser without a centrifuge.

关键词

Keywords

references

林炳承 . 微流控芯片的研究及产业化 . 分析化学 , 2016 . 44 ( 4 ): 491 - 499 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604002 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604002 .

B CH LIN . Research and Industrialization of microfluidic chips . Chinese Journal of Analytical Chemistry , 2016 . 44 ( 4 ): 491 - 499 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604002 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604002 .

郑小林 , 鄢佳文 , 胡宁 , 等 . 微流控芯片的材料与加工方法研究进展 . 传感器与微系统 , 2011 . 30 ( 6 ): 1 - 4, 7 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cgqjs201106001 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cgqjs201106001 .

X L ZHENG , J W YAN , N HU , 等 . Research progress of materials and fabrication methods of microfluidic chip . Transducer and Microsystem Technologies , 2011 . 30 ( 6 ): 1 - 4, 7 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cgqjs201106001 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=cgqjs201106001 .

许雪 , 陈曦 , 赵佳敏 , 等 . 基于3D打印的血型检测微流控芯片研究 . 中国测试 , 2018 . 44 ( 7 ): 68 - 72 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgcsjs201807013 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgcsjs201807013 .

X XU , X CHEN , J M ZHAO , 等 . Research on blood type detection microfluidic chip based on 3D printing . China Measurement & Testing Technology , 2018 . 44 ( 7 ): 68 - 72 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgcsjs201807013 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgcsjs201807013 .

兰子奇 , 史智昊 . 激光技术制备微流道的研究 . 激光杂志 , 2017 . 38 ( 6 ): 124 - 126 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgzz201706031 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgzz201706031 .

Z Q LAN , ZH H SHI . Study on fabrication of microchannel by laser technology . Laser Journal , 2017 . 38 ( 6 ): 124 - 126 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgzz201706031 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgzz201706031 .

王中旺 , 汪帮富 , 丁雯钰 . 超快激光制备PMMA微流道机理及工艺研究 . 激光与红外 , 2019 . 49 ( 8 ): 935 - 940 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgyhw201908005 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgyhw201908005 .

ZH W WANG , B F WANG , W Y DING . Research on ablation mechanism and process parameters of PMMA microchannels by ultrafast laser . Laser & Infrared , 2019 . 49 ( 8 ): 935 - 940 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgyhw201908005 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgyhw201908005 .

陈实 , 张卫平 , 陈文元 , 等 . 用于微流控芯片的PDMS微混合器工艺和数值分析 . 微细加工技术 , 2006 . ( 6 ): 48 - 51, 62 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wxjgjs200606012 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wxjgjs200606012 .

SH CHEN , W P ZHANG , W Y CHEN , 等 . Fabrication and numerical analysis of PDMS micromixer on microfluidic chip . Microfabrication Technology , 2006 . ( 6 ): 48 - 51, 62 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wxjgjs200606012 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=wxjgjs200606012 .

范一强 , 王玫 , 张亚军 . 3D打印微流控芯片技术研究进展 . 分析化学 , 2016 . 44 ( 4 ): 551 - 561 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604008 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604008 .

Y Q FAN , M WANG , Y J ZHANG . Recent progress of 3D printed microfluidics technologies . Chinese Journal of Analytical Chemistry , 2016 . 44 ( 4 ): 551 - 561 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604008 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201604008 .

唐文来 , 樊宁 , 李宗安 , 等 . 基于3D打印牺牲阳模的异型截面微流道便捷加工 . 分析化学 , 2019 . 47 ( 6 ): 838 - 845 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201906006 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201906006 .

W L TANG , N FAN , Z A LI , 等 . Facile fabrication of microchannel with unconventional cross-section using 3D printed sacrificial mould . Chinese Journal of Analytical Chemistry , 2019 . 47 ( 6 ): 838 - 845 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201906006 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fxhx201906006 .

彭子龙, 韦子龙, 刘明杨, 等.电场驱动 μ -3D打印蜡基微流控模具[J/OL].中国机械工程: 1-9[2020-01-02] . http://kns.cnki.net/kcms/detail/42.1294.TH.20191113.1544.040.html http://kns.cnki.net/kcms/detail/42.1294.TH.20191113.1544.040.html .

PENG Z L, WEI Z L, LIU M Y, et al .. Electric-field-driven μ -3D printing wax-based mold for microfluidic[J/OL]. China Mechanical Engineering: 1-9[2020-01-02] . http://kns.cnki.net/kcms/detail/42.1294.TH.20191113.1544.040.html http://kns.cnki.net/kcms/detail/42.1294.TH.20191113.1544.040.html . (in Chinese)

陈昱 . 微流控技术中的微流体控制与应用 . 海峡科技与产业 , 2018 . ( 6 ): 21 - 28 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hxkjycy201806007 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hxkjycy201806007 .

Y CHEN . Microfluidic control and application in microfluidic technology . Technology and Industry Across the Straits , 2018 . ( 6 ): 21 - 28 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hxkjycy201806007 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hxkjycy201806007 .

康明 , 吴建刚 , 曾雪锋 . 基于介质上电润湿的微流体变焦透镜的研究进展 . 光学技术 , 2006 . 32 ( 5 ): 702 - 705 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxjs200605017 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxjs200605017 .

M KANG , J G WU , X F ZENG . Review of variable-focus liquid lenses based on electrowetting on dielectric . Optical Technique , 2006 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxjs200605017 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxjs200605017 .

A A DARHUBER , J P VALENTINO , S M TROIAN , 等 . Thermocapillary actuation of droplets on chemically patterned surfaces by programmable microheater arrays . Journal of Microelectromechanical Systems , 2003 . 12 ( 6 ): 873 - 879 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=599faef2d6223826b6a212f97f93a1b8 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=599faef2d6223826b6a212f97f93a1b8 .

M M AEINEHVAND , F IBRAHIM , S W HARUN , 等 . Biosensing enhancement of dengue virus using microballoon mixers on centrifugal microfluidic platforms . Biosensors & Bioelectronics , 2015 . 67 424 - 430 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=84b4a80249c9b014ac32628cd0fdf6fa http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=84b4a80249c9b014ac32628cd0fdf6fa .

高学金 , 吕昕雨 , 金辰 . 负压型微流控葡萄糖生物传感在线检测系统的研制 . 仪表技术与传感器 , 2019 . ( 9 ): 66 - 68 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ybjsycgq201909015 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ybjsycgq201909015 .

X J GAO , X Y LV , CH JIN . Development of microfluidics glucose biosensing on-line analysis system based on negative pressure driven . Instrument Technique and Sensor , 2019 . ( 9 ): 66 - 68 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ybjsycgq201909015 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ybjsycgq201909015 .

蔡颖, 陈阳, 洪战英, 等.浓度梯度微流控芯片平台的构建及其应用于抗白念珠菌药物快速筛选研究[J/OL].药学学报[2020-01-02] . http://kns.cnki.net/kcms/detail/11.2163.R.20191219.1539.004.html http://kns.cnki.net/kcms/detail/11.2163.R.20191219.1539.004.html .

CAI Y, CHEN Y, HONG ZH Y, et al .. Construction of a concentration gradient microfluidic chip platform for the rapid screening of drugs active against Candida albicans[J/OL]. Acta Pharmaceutica Sinica[2020-01-02] . http://kns.cnki.net/kcms/detail/11.2163.R.20191219.1539.004.html http://kns.cnki.net/kcms/detail/11.2163.R.20191219.1539.004.html . (in Chinese)

P DOW , K KOTZ , S GRUSZKA , 等 . Acoustic separation in plastic microfluidics for rapid detection of bacteria in blood using engineered bacteriophage . Lab on A Chip , 2018 . 18 ( 6 ): 923 - 932 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8a141b88df1a00c8076c47cf26f0d499 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8a141b88df1a00c8076c47cf26f0d499 .

L HUANG , Y CHEN , W HUANG , 等 . Cell pairing and polyethylene glycol (PEG)-mediated cell fusion using two-step centrifugation-assisted single-cell trapping (CAScT) . Lab on A Chip , 2018 . 18 ( 7 ): 1113 - 1120 .

谢晋 , 谭廷武 , 郑佳华 , 等 . 金刚石砂轮V形尖端的数控对磨微细修整技术 . 金刚石与磨料磨具工程 , 2010 . 30 ( 5 ): 1 - 5, 10 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgsymlmjgc201005001 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgsymlmjgc201005001 .

J XIE , Y W TAN , J H ZHENG , 等 . CNC mutual-wear micro truing technique of diamond wheel V-tip . Diamond & Abrasives Engineering , 2010 . 30 ( 5 ): 1 - 5, 10 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgsymlmjgc201005001 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jgsymlmjgc201005001 .

J XIE , H F XIE , M J LUO , 等 . Dry electro-contact discharge mutual-wear truing of micro diamond wheel V-tip for precision micro-grinding . International Journal of Machine Tools and Manufacture , 2012 . 60 44 - 51 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=76e1c870eed893f68f0853938aad3cf1 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=76e1c870eed893f68f0853938aad3cf1 .

J XIE , H H SU , J Y LIAO , 等 . Experimental study on self-flowing speed in microchannel related to micro-/nanoscale surface topographies . Microfluidics and Nanofluidics , 2017 . 21 ( 6 ): 106 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=579b670933fea702616d37469dec0726 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=579b670933fea702616d37469dec0726 .

Views

716

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Nano-reinforced substrate integration in SERS microfluidic chip for bacteria detection

Synergistic bonding process of solvent and tendon for PC-based microfluidic chips

LED induced transmitted fluorescence detector integrated in microfluidic cell chip

Three-dimensional focusing microfluidic chip

Micro positive pressure generator based on AIBN for driving micro fluid

Related Author

Yi XU

Li CHEN

Chuang GE

Hong HE

Shifang LI

WU Yi-hui

WU Jun-feng

LIU Yong-shun

Related Institution

Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment， Chongqing University Cancer Hospital

School of Optoelectronic Engineering， Chongqing University

School of Chemistry and Chemical Engineering， Chongqing University

Key Disciplines Lab of Novel Micro-Nano Devices and System Technology & Key Laboratory of Optoelectronic Technology and Systems， Ministry of Education， Chongqing University

University of Chinese Academy of Sciences

AI问答

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176855 Email：gxjmgc@ciomp.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰