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中国科学院物理研究所凝聚态物理中心, 北京, 100080
收稿日期:2000-08-15,
修回日期:2000-09-01,
网络出版日期:2001-02-15,
纸质出版日期:2001-02-15
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姚新程, 李兆霖, 郭红莲, 程丙英, 张道中. 光阱位置操纵系统的研究[J]. 光学精密工程, 2001,(1): 55-58
YAO Xin-cheng, LI Zhao-lin, GUO Hong-lian, CHENG Bing-ying, ZHANG Dao-zhong. Optical trap steering system[J]. Editorial Office of Optics and Precision Engineering, 2001,(1): 55-58
利用He-Ne激光器
在LEICADMIRBE倒置显微镜上建立了可对生物样品进行三维操纵的光镊系统。在光镊的研制过程中
我们重点对光阱的横向位置操纵系统进行了研究。理论分析及实验表明
利用平移反射镜及转动反射镜的方案均可实现光阱位置的横向操纵。本文对平移反射镜及转动反射镜实现光阱位置操纵的机理进行了详细的论述
并对操纵过程中保证光阱质量不发生变化的条件进行了理论分析和说明。此外
比较分析表明
与平移反射镜系统相比
转镜系统在光镊技术中的应用可加快光镊操纵速度、简化光阱位置操纵系统的复杂程度、有利于系统开发成本的减少及开发周期的缩短。
An optical tweezers
which can be used to mainipulate biological sample in three dimensions
was incorporated into a LEICA DMIRBE inverted microscope with bottom port. During setting up this system
transverse position mainpulating system of an optical trap was studied especially. Theoretical analysis and experimental results indicate that the transvers position of an optical trap can be changed by laterally moving or rotating a mirror. The mechanisms of optical trap steering systems by moving and rotating mirrors were explained in detail
respectively. In addition
comparing with moving mirror system the rotating mirror system is of benefit to increase the mainipulating speed of an optical trap
simplify the mechanism of an optical tweezers
reduce its cost
and shorten the cycle of completing an optical tweezers.
Ishijima A, Kojima H, Tanaka H, Yanagida T. Title simultaneous measurement of in dividual ATPase and mechanical reactions by a single myosin molecule at work[ J]. Optical Review, 1999,6(1):16-23.
Simmons Robert M, Finer Jeffrey T, Chu Steven, Spudich James A. Quantitative measurements of force and displacement using an optical trap[J ]. Biophysical Journal. 1996,(4):1813-1822.
Yao X C, Li Z L, Cheng B Y, Han X H, Zhang D Z. Increasing tra nsverse stability of optical tweezers by using dual-Gaussian beam profile[J]. Chinese Physics, 2000,9(1):65-68.
Ashkin A. Forces of a single-beam gradient laser trap on a di electric sphere in the ray optics regime[J]. Biophys. J.,1992,61(2):569-582.
Wright W H, Sonek G J, Berns M W. Radiation trapping forces on microspheres with optical tweezers[J]. Appl. Phys. Lett, 1993,63(6):715-717.
Simpson N B, McGloin D, Dholakia K, Allen L, Padgett M J. Opti cal tweezers with increased axial trapping efficiency[J]. Journal of Modern O ptics, 1998,45(9):1943-1949.
Chiou A E, Wang W, Sonek G J, Hong J, Berns M W. Interferometr ic optical tweezers[J].Optics Communications, 1997,133(1-6):7-10.
Smith S P, Bhalotra S R, Brody A L, Brown B L, Boyda E K, Pren tiss M. Inexpensive optical tweezers for undergraduate laboratories[J]. Ameri can Journal of Physics, 1990,67(1):26-35.
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