双荧光标记生物芯片激光共聚焦检测系统

马军山; 侯琳琳; 付东翔; 陈家璧; 庄松林

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双荧光标记生物芯片激光共聚焦检测系统

更新时间：2020-08-12

- 双荧光标记生物芯片激光共聚焦检测系统
- Two-laser scanning confocal system for microarray analysis
- 光学精密工程 2005年13卷第6期页码：727-734
- 作者机构：
  
  上海理工大学光学与电子信息工程学院上海,200093
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： TN247
- 收稿日期：2005-06-22，
  
  修回日期：2005-08-18，
  
  网络出版日期：2005-12-30，
  
  纸质出版日期：2005-12-30
- 稿件说明：
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马军山, 侯琳琳, 付东翔, 等. 双荧光标记生物芯片激光共聚焦检测系统[J]. 光学精密工程, 2005,13(6):727-734.

MA Jun-shan, HOU Lin-lin, FU Dong-xiang, et al. Two-laser scanning confocal system for microarray analysis[J]. Optics and precision engineering, 2005, 13(6): 727-734.
马军山, 侯琳琳, 付东翔, 等. 双荧光标记生物芯片激光共聚焦检测系统[J]. 光学精密工程, 2005,13(6):727-734. DOI：

MA Jun-shan, HOU Lin-lin, FU Dong-xiang, et al. Two-laser scanning confocal system for microarray analysis[J]. Optics and precision engineering, 2005, 13(6): 727-734. DOI：

摘要

基于激光共聚焦检测原理

构建了针对荧光标记生物芯片的检测系统

对用Cy

或Cy

标记蛋白点样的玻片进行了扫描检测

并对采集的荧光数据信号进行了图像重建。针对实际荧光信号可能较微弱并存在较大动态范围的情况

采用形态学方法对图像进行滤波、增强处理

显著增强了图像质量。提出了标定系统信噪比和灵敏度的公式

并据此对检测实验结果进行了详细的分析计算

最终标定此激光共聚焦生物芯片检测系统的灵敏度约为0.1 fluo/μm

。

Abstract

A dual-fluorescence microarray scanner based on laser confocal principle was constructed to detect the Cy

-protein or Cy

-protein dotted slides. The images were constructed according to the fluorescent intensity signals

and enhanced through adopting the technique of morphologic methods to meet the situation in which the fluorescent intensity was very low and the dynamic range of the data was wide relatively. The measuring ways of the signal-to-noise ratio and the sensitivity of the system were put forward

and the measurement result shows the sensitivity of the system is about 0.1 fluo/μm

关键词

Keywords

references

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