生物显微切割微操作仪的设计与研制

王会香; 陈立国; 孙立宁; 刘亚欣

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生物显微切割微操作仪的设计与研制

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- 生物显微切割微操作仪的设计与研制
- Design of micromanipulation instrument for bio-microdissection
- 光学精密工程 2006年14卷第3期页码：416-421
- 作者机构：
  
  哈尔滨工业大学机器人研究所,黑龙江哈尔滨150001
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： Q-336
- 收稿日期：2005-11-23，
  
  修回日期：2006-01-20，
  
  网络出版日期：2006-06-30，
  
  纸质出版日期：2006-06-30
- 稿件说明：
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王会香, 陈立国, 孙立宁, 等. 生物显微切割微操作仪的设计与研制[J]. 光学精密工程, 2006,14(3):416-421.

Design of micromanipulation instrument for bio-microdissection[J]. Optics and precision engineering, 2006, 14(3): 416-421.
王会香, 陈立国, 孙立宁, 等. 生物显微切割微操作仪的设计与研制[J]. 光学精密工程, 2006,14(3):416-421. DOI：

Design of micromanipulation instrument for bio-microdissection[J]. Optics and precision engineering, 2006, 14(3): 416-421. DOI：

摘要

设计了一种能够自动、快速、精确地分离和提取指定细胞群或单个细胞的微操作机器人系统

研制了基于功率超声振动原理的微切割工具(其切割频率在15~50 kHz

振动幅值在0~1 μm可调)和真空吸附式微拾取工具(压力可在0~0.5 MPa精确控制)

应用人体肝脏组织5 μm厚病理石蜡切片进行了显微切割的实验。实验结果证明了超声振动式切割工具对组织无伤害

真空吸附工具收集样品有效且整体系统适应于生物工程中微切割操作的实验需求

具有较高的可靠性、稳定性和准确性。

Abstract

This micromanipulation system provides a technology to procure pure population of targeted cells quickly and accurately for pathology research. Essential micromanipulations were executed by microdissection tool based on ultrasonic vibration (vibration frequency and amplitude at 15~50 kHz and 0~1 μm) and microcollection tool was based on vacuum adsorption (controllable pressure at 0~0.5 MPa). A 5 μm liver embedded sections were dissected and collected successfully in the experiments

the contrast testing experiment indicates that the micromanipulation system is applicable to bio-mcirodissection in high stability and accuracy

in which the ultrasonic vibration is no harm to tissue and vacuum adsorption is in good availability to collection samples.

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references

. 闫庆国,黄高升,朱德生,等. 显微切割技术鉴定病毒核内包涵体[J]. 第四军医大学学报,2000,21(1):6-9. YAN Q G., HUANG G S, ZHU D SH, et al. Microdissection technique in the determination of viral intranuclear inclusions[J]. J.Fourth Mil. Med. Univ., 2000,21(1):6-9.(in Chinese)

. BALL H J,MCPARLAND B,DRIUSSI C,et al.Isolating vessels from the mouse brain for gene expression analysisusing laser capture microdissection[J]. Brain Research Protocols,2002,9:206-213.

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. 李旭东,宗光华,毕树生,等. 生物工程微操作机器人视觉系统的研究[J]. 北京航空航天大学学报,2002,28(3):249-252. LI X D, ZHONG G H, BI SH SH. Research on global vision system for bioengineering-oriented micromanipulation robot system [J]. Journal of Beijing University of Aeronautics and Astronautics, 2002,28(3):249-252. (in Chinese)

. ARAI F,AMANO T. Microknife using ultrasonic vibration[J].International Symposium On Micromechatronics And Human Science., 2000 IEEE,200,165-2000.

. ARAI F,AMANO T.Mechanical micro-dissection by microknife using ultrasonic vibration and ultra fine touch probe sensor .International Conference on Robotics, 8 Automation, Seoul , 2001 IEEE, May 21-26.

. ZESCH W,BRUNNER M,WEBE A.Vacuum tool for handling micro objects with a nanorobot .Proceedings of the 1997 IEEE,International Conference on Robotics and Automation,Albuquerque,New Mexico,1997,1761-1766.

. EMMERT F F,BUCK M R,CHUQUI R,et al.Immuno. LCM: laser capture microdissection of immunostained frozen sections for RNA analysis[J]. American Journal of Pathology, 1999, 154(1): 61-66.

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