Angular disturbance isolation is a important function of seeker. The disturbance isolation performance of seeker is often evaluated by isolation index. The principle of disturbance isolation of seeker is analyzed

and the model of isolation index is deduced. By analyzing the structure of stabilization loop of seeker

a isolation index test method based on the dSPACE hardware-in-the-loop simulation system is proposed

which adapt to the stage of controller adjusting. According to add disturbance signal in the stabilization loop using this method

the isolation index is fast tested without simulating missile disturbance by the flight simulation turntable. Compared to the traditional method

this method has simple structure and fast test velocity. Using this method

the isolation index of stabilization loop of a infrared imaging seeker is tested

the results close to the results obtained by using traditional method. It indicates that this method is feasible to test isolation index of seeker or another optoelectronic platform on moving vehicle.