In the protein crystallization

thousands of reagents with different viscosities need to be transferred or dispensed. Most of the commercial automated liquid dispensing systems need to be calibrated through experiment to accurately dispense liquids when the reagent viscosity changes

which is time consuming and less accurate. In this paper

an automated adaptive non contact liquid handling system was developed

which could dispense submicroliter reagent without touching off. The noncontact reagent dispensing is accomplished by rapidly opening and closing the solenoid valve while applying fluid pressure to it and the volume modulation is possible by altering the opening time of the valve and the flow rate. The system combines the advantages of non-contact solenoid-based technology with the robustness and flexibility of traditional liquid handlers. The novel flow-through design and the integration of high-speed flow sensor enable robust

repetitive

flexible

low-volume dispensing of samples with different properties. This paper focuses on the system construction

dispense process analyses and control. Finally

experiments results are presented with different dispensing volumes

coefficient of variance (CV) has been shown to be below 3% at 1μl and approach 8% at 50 nl.