用于微米球形颗粒超分辨尺度测量的背向弹性散射光谱的获取

方晖; 杨爱萍

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用于微米球形颗粒超分辨尺度测量的背向弹性散射光谱的获取

现代应用光学 | 更新时间：2020-08-13

- 用于微米球形颗粒超分辨尺度测量的背向弹性散射光谱的获取
- Acquisition of elastic backscattering spectra for microsphere sizing beyond diffraction limit
- 光学精密工程 2014年22卷第3期页码：547-554
- 作者机构：
  
  南开大学现代光学研究所光学信息技术科学教育部重点实验室天津,300071
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目(No.11074134)();教育部博士点基金资助项目(No.20090031120043)();教育部新世纪优秀人才支持计划资助项目(No.
- DOI：
  中图分类号： O433.4;TB921
- 纸质出版日期：2014-03-25
- 稿件说明：
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方晖,杨爱萍,. 用于微米球形颗粒超分辨尺度测量的背向弹性散射光谱的获取[J]. 光学精密工程, 2014,22(3): 547-554

FANG Hui, YANG Ai-ping,. Acquisition of elastic backscattering spectra for microsphere sizing beyond diffraction limit[J]. Editorial Office of Optics and Precision Engineering, 2014,22(3): 547-554
方晖,杨爱萍,. 用于微米球形颗粒超分辨尺度测量的背向弹性散射光谱的获取[J]. 光学精密工程, 2014,22(3): 547-554 DOI：

FANG Hui, YANG Ai-ping,. Acquisition of elastic backscattering spectra for microsphere sizing beyond diffraction limit[J]. Editorial Office of Optics and Precision Engineering, 2014,22(3): 547-554 DOI：

摘要

背向弹性散射光谱法可用于研究生物细胞内部颗粒的结构组成

本文探讨了背向弹性散射光谱测量获取光谱的不同实验方法及其优缺点。针对微米量级的球形颗粒测量

基于实验分析比较了目前普遍使用的光纤探头测量和透镜系统测量这两种背向散射光谱获取方法的优缺点。研究表明

由于透镜系统获取光谱所对应的散射角有更明确的定义

故透镜系统获取光谱与Mie散射计算的互相关拟合系数(0.96)高于光纤探头优化条件下获取光谱的结果(0.93)。因此

在提取颗粒尺度精度要求较高时

应该选用透镜测量方法

并且采用滤波去噪声信号处理的手段来提高颗粒尺寸提取精度;而提取颗粒尺度精度要求不高时

光纤探头光谱获取方法具有简单易行的优点。

Abstract

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references

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