基于红蓝双光阑的单物镜立体显微成像系统

杨勇; 王宁; 步敬; 袁小聪

doi:null

您当前的位置：

首页 >

文章列表页 >

基于红蓝双光阑的单物镜立体显微成像系统

现代应用光学 | 更新时间：2020-08-13

- 基于红蓝双光阑的单物镜立体显微成像系统
- Single objective stereo microscopy based on red-and-blue dual-apertures
- 光学精密工程 2014年22卷第3期页码：533-538
- 作者机构：
  
  1. 南开大学现代光学所光学信息技术科学教育部重点实验室天津,300071
  2. 深圳大学光电子工程学院微纳光学研究所深圳,518060
- 作者简介：
- 基金信息：
  
  国家973基础研究发展计划资助项目(No.2010CB327702)()
- DOI：
  中图分类号： TP391;TH742.9
- 纸质出版日期：2014-03-25
- 稿件说明：
移动端阅览
杨勇,王宁,步敬等. 基于红蓝双光阑的单物镜立体显微成像系统[J]. 光学精密工程, 2014,22(3): 533-538

YANG Yong, WANG Ning, BU Jing etc. Single objective stereo microscopy based on red-and-blue dual-apertures[J]. Editorial Office of Optics and Precision Engineering, 2014,22(3): 533-538
杨勇,王宁,步敬等. 基于红蓝双光阑的单物镜立体显微成像系统[J]. 光学精密工程, 2014,22(3): 533-538 DOI：

YANG Yong, WANG Ning, BU Jing etc. Single objective stereo microscopy based on red-and-blue dual-apertures[J]. Editorial Office of Optics and Precision Engineering, 2014,22(3): 533-538 DOI：

摘要

为了得到微观动态样品的三维形貌图

提出了基于红蓝双小孔光阑的单物镜立体显微成像方法

并建立了一个结构简单的单物镜立体显微系统。该系统在物镜出瞳处放置分别用红、蓝滤光片覆盖的双小孔光阑

当采用白光照明时

彩色CCD将接收到分别用红光和蓝光表示的两个不同视角的图像。采用数字图像处理方法可获得具有视差的立体像对

基于相位的双目匹配算法可得到样品的三维形貌。实验结果表明

该方法重构了微观样品

其景深达到241.50 m。与现有的双物镜体视显微方法相比

设计的立体显微系统简单且紧凑

利用单次拍摄即可得到微观动态样品的三维形貌图

在显微镜和内窥镜中很有应用前景。

Abstract

To obtain a 3D model of microscopic dynamic samples

a 3D microscopic imaging method based on a single objective and redblue dualapertures was proposed and a corresponding 3D microscopic specimen system for capturing images was established. In the system

the dual apertures covered with a red and a blue filters were respectively placed in the exit pupil plane of the objective. When it was illuminated by a white light

the color CCD in the imaging plane of objective could receive the perspective views of specimen expressed by red and blue images respectively at one exposure. The stereo image with an optical parallax for the specimen could be obtained by digital image processing

and the 3D model of the specimen could be further reconstructed by a phase matching algorithm. Experimental results indicate that the system has reconstructed the 3D model with a Central Depth Plane (CDP) up to 241.50 m.As compared with double-objective 3D microscopic imaging systems

the proposed system is more compact and simpler and can obtain a 3D model of microscopic dynamic sample at one exposure

so that the system has important application prospects on microscopes and endoscopes.

关键词

Keywords

references

唐志列, 梁瑞生, 常鸿森. 双光子和多光子共焦显微镜的成像理论[J].物理学报, 2000, 49(6):1076-1080.

TANG ZH L, LIANG R SH, CHANG H S.The imaging theory of two-photon and multi-photon confocal scanning microscopy[J].Acta Phys.Sin., 2000, 49(6):1076-1080.(in Chinese)

于波, 王立鼎. 一种双目分时显微立体成像系统的研制[J]. 光学精密工程, 1997, 5(4):49-52.

YU B, WANG L D.Development of a binocular field sequential stereo microscopic imaging system[J].Opt. Precision Eng., 1997, 5(4):49-52.(in Chinese)

DAI Z H, WANG J G, ZHAO X, et al..Fast acquisition of 3D images in a modified optical microscope based on integral imaging[J].Science China Technological Sciences, 2012, 55(4):977-981.

邸江磊, 赵建林, 范琦, 等.数字全息显微术中重建物场波前的相位校正[J]. 光学学报, 2008, 28(1):56-61.

DI J L, ZHAO J L, FAN Q, et al..Phase correction of wavefront reconstruction in digital holographic microscopy[J].Acta Optica Sinica, 2008, 28(1):56-61.(in Chinese)

隋婧, 金伟其. 双目立体视觉技术的实现及其进展[J].电子技术应用, 2004, 10(4):4-6.

SUI J, JIN W Q.Realization and development of the binocular stereo vision technology[J].Application of Electronic Technique, 2004, 10(4):4-6.(in Chinese)

GREENING A B, MITCHELL T N.Stereoscopic viewing system using a two dimensional lens system:USA, Patent 5828487[P]. 1998.

MIHALCA G Y, KAZAKEVICH E.Stereoscopic imaging by alternately blocking light :USA,Patent 5964696[P]. 1999.

AMARI Y, ADELSON E H.Single-eye range estimation by using displaced apertures with color filters[J].Motion Control, 1992,3:1588-1592.

BAE S Y, KORNISKI R J, CHOI J M, et al..Development of a miniature single lens dual-aperture stereo imaging system towards stereo endoscopic imaging application[J].Optical Engineering, 2012, 51(10):103202-1-6.

LEVOY M, NG R, ADAMS A,et al..Light field microscopy[J].ACM Transactions on Graphics, 2006, 25(3):924-934.

王昕, 马岩, 杨剑, 等. 一种基于区域立体匹配算法的实现及改进[J].光学精密工程, 2008, 16(10):2002-2008.

WANG X,MA Y,YANG J, et al..Implementation and improvement of area-based stereo matching algorithm[J].Opt. Precision Eng., 2008, 16(10):2002-2008.(in Chinese)

狄红卫, 柴颖, 李逵. 一种快速双目视觉立体匹配算法[J].光学学报, 2009, 29(8):2180-2184.

DI H W, CHAI Y, LI K.A fast binocular vision stereo matching algorithm[J].Acta Optica Sinica, 2009, 29(8):2180-2184.(in Chinese)

LIU J G, YAN H.Phase correlation pixel-to-pixel image co-registration based on optical flow and median shift propagation[J].International Journal of Remote Sensing, 2008, 29(20):5943-5956.

赵杰, 于舒春, 蔡鹤皋.金字塔双层动态规划立体匹配算法[J]. 控制与决策, 2007, 22(1):69-72.

ZHAO J, YU SH CH, CAI H G.Pyramid two-level dynamic programming stereo matching algorithm[J].Control and Decision, 2007, 22(1):69-72.(in Chinese)

张令涛, 曲道奎, 徐方.一种基于图割的改进立体匹配算法[J].机器人, 2010, 32(1):104-108.

ZHANG L T, QU D K, XU F.An improved stereo matching algorithm based on graph cuts[J].Robot, 2010, 32(1):104-108.(in Chinese)

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

共路剪切数字全息自参考聚焦显微成像

机器视觉精密测量中的显微光学聚焦

静态显微光谱成像系统的研制