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重庆理工大学 电子信息与自动化学院,重庆 400050
收稿日期:2009-09-11,
修回日期:2009-10-26,
网络出版日期:2010-08-20,
纸质出版日期:2010-08-20
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赵明富, 钟年丙, 罗彬彬, 胡新宇, 钟连超. 用于生物量浓度在线检测的光纤衰减全反射传感器[J]. 光学精密工程, 2010,18(8): 1707-1714
ZHAO Ming-fu, ZHONG Nian-bing, LUO Bin-bin, HU Xin-yu, ZHONG Lian-chao. Optical fiber attenuated total reflection sensor for on-line measurement of biomass concentration[J]. 光学精密工程, 2010,18(8): 1707-1714
赵明富, 钟年丙, 罗彬彬, 胡新宇, 钟连超. 用于生物量浓度在线检测的光纤衰减全反射传感器[J]. 光学精密工程, 2010,18(8): 1707-1714 DOI: 10.3788/OPE.20101808.1707.
ZHAO Ming-fu, ZHONG Nian-bing, LUO Bin-bin, HU Xin-yu, ZHONG Lian-chao. Optical fiber attenuated total reflection sensor for on-line measurement of biomass concentration[J]. 光学精密工程, 2010,18(8): 1707-1714 DOI: 10.3788/OPE.20101808.1707.
为了在生物膜滴滤塔降解有机废气过程中实现生物量浓度的测量
设计了光纤衰减全反射(ATR) 生物量浓度传感器
给出了传感器组成原理、结构参数、光路分析及其理论解释。根据光波进入光密介质(Si晶体)与光疏介质(生物菌液)的分界面时所产生的隐失波现象
以及生物菌液对隐失波的散射和吸收引起接收光能量变化的物理机理
建立了一种新的生物量浓度测量方法。实验选用可见光源
在25 ℃恒温情况下对生物量浓度测量进行了实验研究。实验结果表明:测量结果的相对误差均值为2.217%
基本上满足生物量浓度在线测量要求。该传感器的设计原理和方法具有一定的普遍意义
可应用于制剂、制酒、生化等行业。
With the aim to measure the biomass concentration in the biodegradation of organic waste gas for a trickling biofilter
an optical fiber Attenuated Total Reflection(ATR) sensor for measuring the biomass concentration was designed and the composition principle
structural parameters
optical path analysis and the theoretical explanation of the sensor were also discussed.A new method to measure the biomass concentration was proposed based on the principles that the evanescent wave is scattered and absorbed by the bacterial suspension when the beams enter into the interface between the optically denser medium-Si crystal and the optically less dense-bacterial suspension
and the receiving bacterial power of the light is changed by the bacterial suspension. Under a temperature of 25℃
the biomass concentration was measured by a visible light source. Experimental results indicate that the mean relative error by proposed method is 2.217%
which can satisfy the on-line measurement requirements of the biomass concentration. The design principle and method of the sensor is significance and can be used in reagent preparation
alcohol production
biochemistry
etc
.
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CHAN K L, GOVADA L, BILL R M, et al.. Attenuated total reflection-FT-IR spectroscopic Imaging of protein crystallization [J]. Analytical Chemistry, 2009,81(10):3769-3775.
孟庆华,向阳. 傅里叶变换光谱仪用红外水平衰减全反射测试仪[J]. 光学精密工程,2007,15(10):1515-1519.
MENG Q H, XIANG Y. Infrared horizontal ATR instrument used in Fourier transform spectroscopy [J]. Opt. Precision Eng., 2007,15(10): 1515-1519. (in Chinese)
龙峰,施汉昌,何苗,等. 倏逝波全光纤免疫传感器的开发及性能研究[J]. 分析化学,2007,35(6):919-923.
LONG F, SHI H C, HE M, et al.. Development and performance study of evanescent wave a11-fiber immunosensor [J]. Chinese Journal of Analytical Chemistry, 2007,35(6):919-923. (in Chinese)
邓立新,冯莹. 光纤倏逝波生物传感器光纤探头的耦合光束限定[J]. 光子学报,2006,35(7):1066-1070.
DENG L X, FENG Y. The probe coupling beam limiting in fiber optic evanescent wave biosensor [J]. Acta Photonica Sinica, 2006,35(7):1066-1070. (in Chinese)
魏建伟,石学法,方习生,等. 菲律宾海三种微微型浮游生物颗粒丰度及其光散射作用[J]. 海洋学报,2008,30(1):105-112.
WEI J W, SHI X F, FANG X S, et al.. Abundances of three pico-planktonic particles and their optical scattering in the Philippine Sea [J]. Acta Oceanologica Sinica, 2008,30(1):105-112. (in Chinese)
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