An scanning surface plasmon resonance biosensor was constructed to quantitatively detect array samples. First
based on the optimal rotation position in plane prism-coupling mode and a double-prism detection optical path
the scanning surface plasmon resonance biosensor for array samples was constructed. Then
the sample density of array samples was calculated. Finally
by taking the distilled water and glucose solution with different concentrations in 5
10
15
20
25
30 and 35 mg/mL as array samples to be measured
the multi-sample spots were detected by surface plasmon resonance method. Experimental results show that the resonance angles of array samples are 73.745
73.919
74.052
74.185
74.306
74.408
74.549 and 74.660
respectively
and the solution concentrations and their resonance angles show a good linear relation
which proves that the proposed method and device are feasibility. The device is significant for increasing the sample density of array samples and realizing the high precision detection.
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references
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