用于多孔板细胞分析的自动显微成像系统

韩西达1; 王敏1; 黄强1; 何永红1; 周超英2

doi:10.3788/OPE.20132110.2543

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用于多孔板细胞分析的自动显微成像系统

现代应用光学 | 更新时间：2020-08-12

- 用于多孔板细胞分析的自动显微成像系统
- Auto-microimaging system for cells analysis with multiple well plates
- 光学精密工程 2013年21卷第10期页码：2543-2548
- 作者机构：
  
  1. 清华大学深圳研究生院光学检测与成像实验室,广东深圳,518055
  2. 哈尔滨工业大学深圳研究生院机电工程与自动化学院,广东深圳,518055
- 作者简介：
- 基金信息：
- DOI：10.3788/OPE.20132110.2543
  中图分类号： TH703;TH742.9
- 收稿日期：2013-05-08，
  
  修回日期：2013-06-17，
  
  网络出版日期：2012-10-19，
  
  纸质出版日期：2013-10-15
- 稿件说明：
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韩西达王敏黄强何永红周超英. 用于多孔板细胞分析的自动显微成像系统[J]. 光学精密工程, 2013,21(10): 2543-2548

HAN Xi-da WANG Min HUANG Qiang HE Yong-hong ZHOU Chao-ying. Auto-microimaging system for cells analysis with multiple well plates[J]. Editorial Office of Optics and Precision Engineering, 2013,21(10): 2543-2548
韩西达王敏黄强何永红周超英. 用于多孔板细胞分析的自动显微成像系统[J]. 光学精密工程, 2013,21(10): 2543-2548 DOI： 10.3788/OPE.20132110.2543.

HAN Xi-da WANG Min HUANG Qiang HE Yong-hong ZHOU Chao-ying. Auto-microimaging system for cells analysis with multiple well plates[J]. Editorial Office of Optics and Precision Engineering, 2013,21(10): 2543-2548 DOI： 10.3788/OPE.20132110.2543.

摘要

设计了用于多孔板细胞分析的自动显微系统以实现药物筛选的自动检测。研究了系统的机械结构、人机交互软件、对焦评价函数的选择和多视野图像拼接功能。该系统采用支架式结构，克服了形变带来的精度影响；对比传统机械传动的优缺点后，采用伺服电机和音圈电机两级调节的方式来提高系统精度和对焦速度。比较了几种对焦评价函数的性能并提出性能更优的评价方法。考虑到高倍显微镜下视野小对实验统计意义的影响，设计了多视野图像拼接等图像处理功能。最后，实验验证了系统的设计技术指标。实验结果表明：单次对焦时间为2 s；对焦精度在微米级；单次44图像阵列拼接时间为15s

满足系统进行药物筛选对速度、自动化程度和稳定可靠性的要求。

Abstract

An auto-microimaging system to provide 3D scanning and auto-focus at each dimension was designed for the high throughput micro-imaging of drug screening. The mechanical structure

man-machine interaction software

the section of focusing evaluation function and the image titching of multi-view image for the system were researched. A rack structure was designed to overcome the limitation of accuracy from the deformation and a servo motor and a voice coil motor were combined as a two-stage controller to improve the accuracy and focus speed of the measuring system. Then

an algorithm of auto-focus was presented after several auto-focus evaluating methods were compared. According to the effect of a small view under the high power microscope on the experimental statistics

the function of image stitching was designed to fit the statistical significance of the experiment. On the experimental verification

it indicates that the time to complete the process of auto-focus is 2 s

and the accuracy of auto-focus can reach several micrometers. Moreover

the time to combine the micro-imaging regions of a 44 array is 15 s. The system can satisfy the requirements of short detection time

robotization

stability and reliability of cell analyzing with multiple well plates in drug screening.

关键词

Keywords

references

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