全血微流控芯片的高灵敏度多参数光探测

周文超1; 吴一辉1; 郝鹏1; 刘桂根1; 刘永顺1

doi:10.3788/OPE.20132111.2821

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全血微流控芯片的高灵敏度多参数光探测

微纳技术与精密机械 | 更新时间：2020-08-12

- 全血微流控芯片的高灵敏度多参数光探测
- Highly sensitive and multi-parameter optical detection for whole blood on centrifugal microfluidic chip
- 光学精密工程 2013年21卷第11期页码：2821-2828
- 作者机构：
  
  1. 中国科学院大学北京,中国,100049
  2. 中国科学院长春光学精密机械与物理研究所,吉林长春,130033
- 作者简介：
- 基金信息：
  
  吉林省科技发展计划项目(20140203011GX)
- DOI：10.3788/OPE.20132111.2821
  中图分类号： O661.1;TH741
- 收稿日期：2012-07-11，
  
  修回日期：2012-10-29，
  
  网络出版日期：2013-11-22，
  
  纸质出版日期：2013-11-15
- 稿件说明：
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周文超, 吴一辉, 郝鹏, 刘桂根, 刘永顺. 全血微流控芯片的高灵敏度多参数光探测[J]. 光学精密工程, 2013,21(11): 2821-2828

ZHOU Wen-Chao, TUN Yi-Hui, HAO Feng, LIU Gui-Gen, LIU Yong-Shun. Highly sensitive and multi-parameter optical detection for whole blood on centrifugal microfluidic chip[J]. Editorial Office of Optics and Precision Engineering, 2013,21(11): 2821-2828
周文超, 吴一辉, 郝鹏, 刘桂根, 刘永顺. 全血微流控芯片的高灵敏度多参数光探测[J]. 光学精密工程, 2013,21(11): 2821-2828 DOI： 10.3788/OPE.20132111.2821.

ZHOU Wen-Chao, TUN Yi-Hui, HAO Feng, LIU Gui-Gen, LIU Yong-Shun. Highly sensitive and multi-parameter optical detection for whole blood on centrifugal microfluidic chip[J]. Editorial Office of Optics and Precision Engineering, 2013,21(11): 2821-2828 DOI： 10.3788/OPE.20132111.2821.

摘要

针对微流控芯片尺寸小引起吸光度检测方法中光程短的问题，提出了基于类法布里-珀罗（F-P）共振腔的全反射光探测结构并采用离心驱动方法实现了微流控芯片上全血的高灵敏度多参数分析。首先，理论分析了取得最大光程和最佳信噪比的条件，分析表明：探测池体积一定时，理论最大光程仅与入射光斑直径平方成反比，信噪比与反射次数与反射率有关。然后，优化设计了一探测池结构，通过检测亚甲基蓝溶液实验验证了所设计探测池结构的灵敏度，其光程提高了1~2个量级。最后，在芯片上检测全血中的白蛋白和葡萄糖两种物质，结果表明，亚甲基蓝溶液、白蛋白试剂、葡萄糖试剂3种物质的线性相关系数分别为0.999 07、0.993 94、0.992 87，线性度很好，为微流控芯片实现全集成全血的多参数测量奠定了基础。

Abstract

The absorbance measurement method has a shorter optical path due to the microfluidic chip with smaller sizes. Therefore

a multireflection structure based on a quasi Fabry-Perot cavity was proposed to enhance the optical path length and complete the highly sensitive and multi-parameter detection for the whole blood on the centrifugal microfluidic chip. First

the optimal condition of the largest path length and the proper Signal and Noise Ratio(SNR) were analyzed theoretically. The results indicate that the maximum path length is only inversely proportional to the square of the beam diameter and the SNR is dependent on the reflectivity of the mirror and the number of reflections. Then

a detection pool was designed

the structure sensitivity of the pool was verified by the methylene blue solutions with different molar concentrations

and its path length was proved to be improved by two orders. Finally， the albumin and glucose in the whole blood on the centrifugal microfluidic platform was measured

and the good linearity with typical R2(linear relation coefficient) values of 0.999 07

0.993 94

and 0.992 87 were obtained. From these results

it concludes that the quasi Fabry-Perot cavity is suitable for the whole blood biochemical detection and can satisfy the requirements of accuracy.

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references

YAGER P, EDWARDS T, FU E, et al.. Microfluidic diagnostics technologies for global public health [J]. Nature,2006,442(7101):412-418.[2]MAO X L,HUANG T J.Microfludic diagnostics for the developing world [J].Lab on a chip,2012,8(12):1412-1416.[3]杜新，张平，刘永顺，等.基于PDMS和玻璃材料的临界管被动阀临界压力分析[J].光学精密工程，2011,19(8):1852-1858.DU X, ZHANG P,LIU Y SH, et al.. Burst pressure of capillary burst valve based on glass and PDMS [J].Opt. Precision Eng.,2011,19(8):1852-1858.(in Chinese)[4]邓永波，张平，杜新，等.亲/疏水性不同壁面组成微通道的深宽比与通道内液体的自发毛细流动[J].光学精密工程，2010,18 (7)：1562-1567.DENG Y B,ZHANG P,DU X, et al.. Aspect ratio for microchannels with nonuniform surface properties and spontaneous capillary[J]. Opt. Precision Eng.,2010,18(7):1562-1567.(in Chinese)[5]张平，胡亮红，吴一辉，等.主辅通道型微混合器的设计与制作[J].光学精密工程，2010,18(4)：872-879.ZHANG P,HU L H,WU Y H, et al.. Design and fabrication of micro mixer with main-assist channels[J]. Opt. Precision Eng.,2010,18(4):872-879.(in Chinese)[6]张平，徐磊，邓永波.微通道内台阶阀截止过程中的毛细流动动态效应[J].光学精密工程，2011,19(12)：2919-2926.ZHANG P,XU L,DENG Y B. Dynamic effect in process of stopping capillary by step value in microchannel[J]. Opt. Precision Eng.,2011,19(12):2919-2926.(in Chinese)[7]STEIGERT J,GRUMANN M,BRENNER T,et al.. Integrated sample preparation,reaction and detection on a high-frequency centrifugal microfluidic platform[J]. The Journal of Academic Librarianship,2005,10(5):331-341.[8]CHO Y K, LEE J G, PARK J M, et al..One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device[J].Lab on a chip,2007,7(5):565-573.[9]LEE B S, LEE Y U, KIM H S, et al..Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood [J].Lab on a chip,2011,11(1):70-78.[10]PiCCOLO,ABAXIS Inc..[BE/OL].http://www.abaxis.com.[11]ANALYST Ⅱ、Ⅲ，HEMAGEN Inc..[EB/OL].http://www.hemagen.com.[12]PAN J Z, YAO B,FANG Q..Hand-held photometer based on liquid-core waveguide absorption detection for nanoliter-scale samples[J].Analytical Chemistry,2010,82(8):3394-3398.[13]MA J,DASGUPTA P K,BLACKLEDGE W,et al..Cobinamide-based cyanide analysis by multiwavelength spectrometry in a liquid core waveguide[J]. Analytical Chemistry,2010,82(14):6244-6250.[14]STEIGERT J, GRUMANN M,BRENNER T, et al.. Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform [J].Lab on a chip,2006,6(8):1040-1044.[15]NODA T, TAKAO H, YOSHIOKA K, et al..Performance of absorption photometry microchip for blood hemoglobin measurement integrated with processing circuits and Si(110) 45 degrees mirrors[J].Sensors and Actuators B：Chemical,2006,119 (1):245-250.[16]MOGENSEN K B, PETERSEN N J, HUBNER J, et al.. Monolithic integration of optical waveguides for absorbance detection in microfabricated electrophoresis devices[J].Electrophoresis,2001,22(18):3930-3938.[17]RO K W, LIM K, SHIM B C,et al.. Integrated light collimating system for extended optical-path-length absorbance detection in microchip-based capillary electrophoresis[J]. Analytical Chemistry,2005,77(16):5160-5166.[18]吴一辉，张平，邓永波，等.多功能集成式微流控芯片及其制作方法：中国，CN101907629A[P]. 2010.WU Y H, ZHANG P, DENG Y B,et al.. Multi-function integrated microfluidic chip and the method of fabrication：China,CN101907629A[P]. 2010.(in Chinese)[19]STEPHENS E R. Long-path infrared spectroscopy for air pollution research [J].Applied Spectroscopy,1958,12(3):80-84.[20]WERLE P, SLEMR F. Signal-to-noise ratio analysis in laser absorption spectrometers using optical multipass cells [J]. Applied Optics,1991,30(4):430-434.

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