CD-like全血分析芯片内压缩空气的定量泵送

范建华; 邓永波; 宣明; 周松; 武俊峰; 刘永顺; 吴一辉

doi:10.3788/OPE.20142210.2733

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CD-like全血分析芯片内压缩空气的定量泵送

微纳技术与精密机械 | 更新时间：2020-08-13

- CD-like全血分析芯片内压缩空气的定量泵送
- Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis
- 光学精密工程 2014年22卷第10期页码：2733-2739
- 作者机构：
  
  1. 中国科学院长春光学精密机械与物理研究所,吉林长春,中国,130033
  2. 中国科学院大学北京,中国,100049
- 作者简介：
- 基金信息：
  
  国家863高技术研究发展计划资助项目(No.2012AA040503)();国家自然科学基金资助项目(No.5120538)()
- DOI：10.3788/OPE.20142210.2733
  中图分类号： Q-33;TQ022.1
- 收稿日期：2013-12-07，
  
  修回日期：2014-01-20，
  
  纸质出版日期：2014-10-25
- 稿件说明：
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范建华, 邓永波, 宣明等. CD-like全血分析芯片内压缩空气的定量泵送[J]. 光学精密工程, 2014,22(10): 2733-2739

FAN Jian-hua, DENG Yong-bo, XUAN Ming etc. Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis[J]. Editorial Office of Optics and Precision Engineering, 2014,22(10): 2733-2739
范建华, 邓永波, 宣明等. CD-like全血分析芯片内压缩空气的定量泵送[J]. 光学精密工程, 2014,22(10): 2733-2739 DOI： 10.3788/OPE.20142210.2733.

FAN Jian-hua, DENG Yong-bo, XUAN Ming etc. Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis[J]. Editorial Office of Optics and Precision Engineering, 2014,22(10): 2733-2739 DOI： 10.3788/OPE.20142210.2733.

摘要

为了克服离心式微流控芯片上血清提取对虹吸管亲水性的依赖

保证离心式微流控芯片功能的稳定性和可靠性

在芯片上设计了连接于血液分离腔的压缩空气腔

实现了高转速下血液的分离.基于压缩空气的辅助作用

并通过转速降低导致压缩空气腔内所储存气体压强的释放对血清的泵送作用

实现了离心式微流控芯片上血清的定量提取.基于等温气体的热力学平衡理论

分析了压缩空气腔压缩体积和虹吸管内液面位置与电机转速之间的关系

给出了基于压缩空气辅助作用的离心式血清提取结构的设计规律.以聚甲基丙烯酸甲酯(PMMA)为基材

采用CO

激光加工工艺

制作了离心式血清提取芯片

并测试了不同转速下被压缩气体的体积和血清液面在虹吸管中的位置.实验结果表明:转速为4000 r/min时

空气的被压缩量为8.7 μL

虹吸管能有效抑制全血溢出以防止全血进入血清提取腔;当转速降为1 000 r/min时

压缩气体所储存压强得到释放

克服了离心力

并驱动血清流过虹吸管最高点

进而实现血清的定量提取.

Abstract

To overcome the dependence of the plasma extraction on siphon hydrophilicity in a centrifugal microfluidic chip and to ensure long-term stability and reliability of the microfluidic chip

a compressed air reservoir connected to the blood separated reservoir was proposed to separate the blood in blood separated reservoir at high rotating speeds. Through lowering the spinning speed

the plasma in the separated reservoir was pumped toward the CD-like chip center to release the stored air pressure produced by the rotation at high spinning speeds. Based on the thermodynamics of the isothermal gas

the pneumatic pumping method was modeled

and the model was confirmed by analyzing pumping positions and air compression versus spinning speeds. By using the layered Polymethyl Methacrylate(PMMA)as materials

the centrifugal microfluidic chips for plasma extraction were fabricated with a CO

laser process technique and the volume of compressed air and the blood position at siphon were tested at different rotational speeds. Experimental results demonstrate that the achieved volume of compression is 8.7μL and the siphon valve effectively inhibits whole blood to overflow the crest of siphon at 4 000 r/min speed. With lowering the spin speed to 1 000 r/min

the release of compressed air overcomes the centrifugal force and drives the plasma to flow past the crest of siphon. Then the plasma extraction is realized in quantitation.

关键词

Keywords

references

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