多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器

何皓; 张涛; 姚佳; 李传宇; 周连群; 王毅; 李明宇; 黎海文; 董文飞

doi:10.3788/OPE.20152303.0723

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多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器

微纳技术与精密机械 | 更新时间：2020-08-13

- 多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器
- Surface plasmon resonance sensor coated with poriferous MIPs for microcystin-LR detection
- 光学精密工程 2015年23卷第3期页码：723-728
- 作者机构：
  
  1. 中国科学院长春光学精密机械与物理研究所,吉林长春,中国,130033
  2. 中国科学院大学北京,中国,100049
  3. 中国科学院苏州生物医学工程技术研究所,江苏苏州,中国,215163
  4. 南洋理工大学仿生传感器科学中心新加坡,637553
  5. 浙江大学光电信息工程学系,浙江杭州,310027
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目(No.51202154,No.51205268,No.91123029,No.61340032)()
- DOI：10.3788/OPE.20152303.0723
  中图分类号： X832;TP212.3
- 收稿日期：2014-06-11，
  
  修回日期：2014-08-09，
  
  纸质出版日期：2015-03-25
- 稿件说明：
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何皓, 张涛, 姚佳等. 多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器[J]. 光学精密工程, 2015,23(3): 723-728

HE Hao, ZHANG Tao, YAO Jia etc. Surface plasmon resonance sensor coated with poriferous MIPs for microcystin-LR detection[J]. Editorial Office of Optics and Precision Engineering, 2015,23(3): 723-728
何皓, 张涛, 姚佳等. 多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器[J]. 光学精密工程, 2015,23(3): 723-728 DOI： 10.3788/OPE.20152303.0723.

HE Hao, ZHANG Tao, YAO Jia etc. Surface plasmon resonance sensor coated with poriferous MIPs for microcystin-LR detection[J]. Editorial Office of Optics and Precision Engineering, 2015,23(3): 723-728 DOI： 10.3788/OPE.20152303.0723.

摘要

开发了一种多孔分子印迹膜修饰的表面等离子体共振(SPR)传感器

用于快速检测水中微囊藻毒素LR。研究了利用该传感器检测微囊藻毒素LR的方法。首先

通过原位聚合法在SPR传感芯片的裸金表面合成了微囊藻毒素LR的多孔分子印迹膜

制备出可以特异性捕获微囊藻毒素LR的SPR传感芯片。然后

利用Kretschmann棱镜耦合结构

构建了基于Kretschmann结构的波长调制型表面等离子体共振传感器。最后

通过检测不同浓度的微囊藻毒素LR溶液以及干扰物质微囊藻毒素RR溶液

研究了该传感器的测量范围、特异性等参数。结果表明

该传感器对于微囊藻毒素LR的检测灵敏度很高

可实现微囊藻毒素LR的定量检测

动态测量达2.1×10

-9

~1×10

-6

mol/L。另外

传感器对于干扰物质微囊藻毒素RR无明显信号响应

表明传感器对于微囊藻毒素LR具有很好的特异性检测能力。

Abstract

A novel surface plasmon resonance (SPR) sensor coated with poriferous molecularly imprinted polymers (MIPs)was developed to detect the trace microcystin-LR in water. The method to detect the trace microcystin-LR in water was explored. Firstly

the in situ self-assembly method was used to synthesize a poriferous thin MIPs film on the gold-plated glass. The SPR chip to specifically capture the microcystin-LR was obtained. Then

based on the typical Kretschmann prism coupling structure

a new type of SPR sensor based on wavelength interrogation was constructed by utilizing the MIPs coated gold-plated glass as the sensing chip. The sensor was used to detect different concentration of microcystin-LR solutions and microcystin-RR solutions and to obtain its measuring ranges and specificity parameters. The result indicates that the sensor is sensitive to microcystin-LR and completes a quantitative determination with a dynamic measure range of 2.1×10

-9

—1×10

-6

mol/L. Moreover

the sensor did not have obvious response to microcystin-RR

the analogues of microcystin-LR. It means that the sensor has special selectivity to recognize the microcystin-LR.

关键词

Keywords

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