激光共焦显微光束的偏转扫描

赵维谦; 任利利; 盛忠; 王允; 邱丽荣

doi:10.3788/OPE.20162406.1257

您当前的位置：

首页 >

文章列表页 >

激光共焦显微光束的偏转扫描

现代应用光学 | 更新时间：2020-08-13

- 激光共焦显微光束的偏转扫描
- Beam deflection scanning for laser confocal microscopy
- 光学精密工程 2016年24卷第6期页码：1257-1263
- 作者机构：
  
  北京理工大学光电学院北京,中国,100081
- 作者简介：
- 基金信息：
  
  国家自然基金优秀青年科学基金资助项目（No.51422501）();国家自然基金面上项目（No.61475020）()
- DOI：10.3788/OPE.20162406.1257
  中图分类号： TH742;TN409
- 收稿日期：2016-03-22，
  
  修回日期：2016-04-12，
  
  纸质出版日期：2016-06-25
- 稿件说明：
移动端阅览
赵维谦, 任利利, 盛忠等. 激光共焦显微光束的偏转扫描[J]. 光学精密工程, 2016,24(6): 1257-1263

ZHAO Wei-qian, REN Li-li, SHENG Zhong etc. Beam deflection scanning for laser confocal microscopy[J]. Editorial Office of Optics and Precision Engineering, 2016,24(6): 1257-1263
赵维谦, 任利利, 盛忠等. 激光共焦显微光束的偏转扫描[J]. 光学精密工程, 2016,24(6): 1257-1263 DOI： 10.3788/OPE.20162406.1257.

ZHAO Wei-qian, REN Li-li, SHENG Zhong etc. Beam deflection scanning for laser confocal microscopy[J]. Editorial Office of Optics and Precision Engineering, 2016,24(6): 1257-1263 DOI： 10.3788/OPE.20162406.1257.

摘要

为了提高激光共聚焦系统的扫描速度，本文提出一种逐场扫描的场同步扫描方法。构建了激光共焦显微系统，将美国THORLABS公司的GVS002型二维检流计振镜应用于该系统，根据光学系统参数以及扫描范围要求计算振镜的整场扫描波形。借助NI公司的PCIe6353多功能数据采集卡，输出行同步的扫描波形，同时，对共焦显微系统共焦位置上针孔处的光强信号进行采集，先后扫描一幅256×256和512×512的图像，记录扫描图像和成像时间；然后，在相同的硬件结构下，以场同步的方式输出扫描波形，记录扫描图像和成像时间。实验结果表明：场同步方式扫描256×256图像的速度可提高10倍，扫描512×512图像的速度可提高5倍，且满足共焦显微成像的清晰、抗干扰能力强等要求。与行同步扫描方法相比，场同步扫描方法可以消除行与行之间转换的停留时间，在不改变硬件的情况下大幅提高扫描速度。

Abstract

To improve the scanning speed of the laser confocal system

a field synchronous scanning method was proposed. The laser confocal microscopy system was implemented with a GVS002 two-dimensional galvanometer from the THORLABS Company. The scanning waveforms of galvanometer were calculated in accordance with the parameters and scanning range of the optical system. The synchronous scanning waveform was output from a PCIe6353 multi-functional data acquisition card of NI Company. Meanwhile

it collected the optical intensity signals at the pinhole at the focus of the confocal microscopy system and scans a 256×256 image and a 512×512 image successively

recording the scanning images and imaging time; then

the scanning waveforms were output in field synchronization under the same hardware structures

recording the scanning images and imaging time. The experimental results show that the speed of the 256×256 image scanning through field synchronization can be increased by 10 times and the speed of the 512×512 image scanning can be increased by 5 times. In addition the field synchronous scanning can meet such requirements of the confocal microscopy imaging as the clearness and strong capacity of resisting disturbance. Compared with the line synchronization scanning

the field synchronous scanning can eliminate the retention time during the line to line conversion and significantly improve the scanning speed without changing the hardware.

关键词

Keywords

references

邱丽荣,李佳,赵维谦,等. 激光共焦透镜曲率半径测量系统[J]. 光学精密工程,2013,21(2):246-252. QUI L R, LI J, ZHAO W Q, et al.. Laser confocal measurement system for curvature radii of lenses [J]. Opt. Precision Eng., 2013, 21(2): 246-252. (in Chinese)

郭俊杰,邱丽荣,王允,等. 用于惯性约束聚变靶丸测量的激光差动共焦传感器[J]. 光学精密工程,2013,21(3):644-651. GUO J J, QIU L R, WANG Y, et al.. Laser differential confocal sensor for ICF capsule measurement [J]. Opt. Precision Eng., 2013, 21(3): 644-651. (in Chinese)

肖卉,张德艳,范忠义. 感染性角膜炎466例共焦显微镜检查结果临床分析[J]. 国际眼科杂志,2014(10):1825-1827. XIAO H, ZHANG D Y,FAN ZH Y. Clinical features and confocal microscopic imaging characteristics of 466 cases with infectious keratitis [J]. International Eye Science,2014(10):1825-1827. (in Chinese)

宋树权,左敦稳,赵世田. 基于激光扫描共焦显微技术的刀具刃口精密测量[J]. 华南理工大学学报:自然科学版,2014(7): 86-90, 103. SONG SH Q,ZOU D W,ZHAO SH T. Precision measurement of cutting edge based on laser scanning confocal microscopy [J]. Journal of South China University of Technology:Natural Science Edition, 2014(7):86-90,103. (in Chinese)

HUNTER J J, COOKSON C J, KISILAK M L, et al.. Characterizing image quality in a scanning laser ophthalmoscope with differing pinholes and induced scattered light[J]. Journal of the Optical Society of America A-Optics Image Science and Vision, 2007, 24(5):1284-1295.

胡茂海,杨晓春. 共聚焦显微镜激光高速扫描控制系统设计及实现[J]. 应用光学,2011, 32(4):797-800. HU M H, YANG X CH. High-speed laser scanning control system for confocal microscopy [J]. Journal of Applied Optics, 2011, 32(4): 797-800. (in Chinese)

林晓东,蔡红红,胡居广,等. 振镜扫描光谱仪用于托卡马克等离子体谱线轮廓测量[J]. 光电子·激光,2004, 15(4):502-504. LIN X D,CAI H H,HU J G, et al.. Measurement of spectral profiles in Tokamak plasma with an optical scanner spectrometer [J]. Journal of Optoelectronics·Laser,2004, 15(4):502-504. (in Chinese)

黄冬林,杨永强. 基于振镜扫描的激光点焊形貌分析[J]. 应用激光,2009,29(3):199-202, 228. HUANG D L,YANG Y Q. An experimental study on spot shape based on galvanometer scanning laser spot welding [J]. Applied Laser, 2009,29(3):199-202, 228. (in Chinese)

李旭东,崔磊,赵慧洁,等. 双振镜点扫描三维形貌测量系统[J]. 光学精密工程,2010, 18(7): 1648-1653. LI X D, CUI L, ZHAO H J, et al.. Three-dimensional shape measurement system based on dual oscillating mirrors with point scanning[J]. Opt. Precision Eng., 2010, 18(7): 1648-1653. (in Chinese)

张平,陈德,吴震,等. 共焦激光扫描荧光显微镜的扫描系统研究[J]. 应用光学,1995(3):33-38. ZHANG P,CHEN D,WU ZH, et al.. Research on scanning system of confocal laser scanning fluorescence microscope [J]. Applied Optics,1995(3):33-38. (in Chinese)

李鹏,范璐璐. 小型化振镜扫描器电路设计[J]. 激光与红外, 2009(11): 1193-1197. LI P,FAN L L. Circuit design of miniature vibrating mirror scanner [J].Laser and Infrare,2009(11): 1193-1197. (in Chinese)

陈群. 双振镜激光扫描系统的FPGA接口板设计[D]. 成都:电子科技大学, 2015. CHEN Q. The Design of FPGA Interface Board of Dual Galvanometer Scanning System [D].Chengdu:University of Electronic Science and Technology of China, 2015. (in Chinese)

徐亮亮. 二维激光振镜扫描控制系统设计[D]. 长春:长春理工大学, 2012. XU L L. Design of Two Dimensional Laser Scanning Control System [D].Changchun:Changchun University of Science and Technology, 2012. (in Chinese)

HAYAKAWA T, WATANABE T, ISHIKAWA M. Real-time high-speed motion blur compensation system based on back-and-forth motion control of galvanometer mirror[J]. Optics Express, 2015, 23(25): 31648-31661.

GAO C, LIU D, ZHAO W. The distortion analysis and correction of two-dimensional scanning system in laser differential confocal microscopy[J]. SPIE,2012,8857(1):835-838.

浏览量

416

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据