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1.河北工业大学 电气工程学院, 天津 300401
2.天津大学 精密仪器与光电子工程学院, 天津 300072
3.天津科技大学 食品工程与生物技术学院, 天津 300457
[ "董国亚 (1971-), 女, 河北石家庄人, 博士, 副教授, 硕士生导师, 1994年于河北工业大学获得学士学位, 1999年于北京理工大学获得硕士学位, 2003年于清华大学获得博士学位, 主要从事生物医学信号处理, 生物电磁场计算和光电信号检测等方面的研究。E-mail:dongguoya71@163.comd" ]
孙传强 (1988-), 男, 山东潍坊人, 博士研究生, 2009年于山东大学获得学士学位, 2012年于天津大学获得硕士学位, 主要从事质谱、光谱检测技术与仪器方面的研究。E-mail:suncq@tju.edu.cn SUN Chuan-qiang, E-mail:suncq@tju.edu.cn
收稿日期:2016-08-01,
录用日期:2016-9-6,
纸质出版日期:2017-03-25
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董国亚, 赵翔, 张燕, 等. 便携式上转换荧光试纸条检测仪的研制[J]. 光学 精密工程, 2017,25(3):584-590.
Guo-ya DONG, Xiang ZHAO, Yan ZHANG, et al. Development of portable up-conversion photoluminescence strip detector[J]. Optics and precision engineering, 2017, 25(3): 584-590.
董国亚, 赵翔, 张燕, 等. 便携式上转换荧光试纸条检测仪的研制[J]. 光学 精密工程, 2017,25(3):584-590. DOI: 10.3788/OPE.20172503.0584.
Guo-ya DONG, Xiang ZHAO, Yan ZHANG, et al. Development of portable up-conversion photoluminescence strip detector[J]. Optics and precision engineering, 2017, 25(3): 584-590. DOI: 10.3788/OPE.20172503.0584.
为了实现对上转换荧光试纸条的定量检测,研制了便携式上转换荧光试纸条检测仪,并通过实际测试验证了仪器的主要性能。首先,介绍了上转换荧光技术以及上转换荧光试纸条的测试原理,描述了以光学扫描检测系统和电路系统为核心的便携式荧光试纸条检测仪的设计方案,能够将试纸条的荧光信号转变为电信号并进行处理;然后,基于设计方案研制了检测仪样机,并对实际检测试纸条采集到的信号进行优化;最后,对检测仪的性能进行了测试。测试结果表明:仪器稳定、重复性好,样本检测标准差小于0.004;线性响应特性良好,拟合出的浓度标准曲线决定系数为0.996 3。该检测仪结构精巧、功耗低、性能良好,很好地满足了上转换荧光试纸条定量检测的需求。
A portable up-conversion fluorescent strip detector was developed to achieve the quantitative analysis of the up-conversion fluorescent strip. The performance of the detector was verified by practical measurements. The up-conversion fluorescent technology and the principle of up-conversion fluorescent strip were introduced firstly. The up-conversion fluorescent strip detector contained two critical components that were optical scanning system and electrical system
enabling the conversion of fluorescence signals to electric signals. Then
the prototype of the detector was developed and tested experimentally
therein the sampled signals detected by the strip were optimized. This apparatus proves great stability and repeatability
and the standard deviation of the detection is less than 0.004. The linear response characteristic is also excellent as the coefficient of determination of concentration standard curve reaches 0.996 3. This detector has a low power consumption and high performance with an exquisite structure
thus satisfying the requirements of quantitative analysis through up-conversion fluorescent strip.
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李向丽, 谭贵良, 张娜, 等.上转换发光纳米技术及其在食品安全检测中应用研究进展[J].现代食品科技, 2014, 30(8):280-287.
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Wu T H, MAO J W, CHEN F, et al.. Rapid trace microbia detection system [J].Opt. Precision Eng., 2015, 23(11): 3061-3068. (in Chinese)
LI X T, JIANG K, WANG P. Research of quantitative analyzer for fluorescence immune chromatography strips[J].Applied Mechanics & Materials, 2013, 373-375: 1000-1006.
顾雯雯.微流控细胞芯片LED诱导透射式荧光检测微系统[J].光学 精密工程, 2014, 22(8):2159-2165.
Gu W W. LED induced transmitted fluorescence detector integrated in microfluidic cell chip [J]. Opt. Precision Eng., 2014, 22(8): 2159-2165. (in Chinese)
李庆扬, 王能超, 易大义.数值分析[M].第五版.北京:清华大学出版社, 2014.
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肖友益.化学发光免疫分析标准曲线拟合模式选择[J].现代检验医学杂志, 2009, 24(5):157.
XIAO Y Y. Selection of chemiluminescence immune assay standard curve fitting model [J]. Journal of Modern Laboratory Medicine, 2009, 24(5): 157. (in Chinese)
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