发光染料罗丹明B的荧光传感特性

张巍巍; 史凯兴; 赵小兵; 秦朝菲

doi:10.3788/OPE.20172503.0591

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发光染料罗丹明B的荧光传感特性

现代应用光学 | 更新时间：2020-07-07

- 发光染料罗丹明B的荧光传感特性
- Multi-functional fluorescent sensing properties of rhodamine B
- 光学精密工程 2017年25卷第3期页码：591-596
- 作者机构：
  
  南昌航空大学江西省光电检测技术工程实验室, 江西南昌 330063
- 作者简介：
  
  [ "张巍巍 (1974-), 男, 湖北黄梅人, 副教授, 研究生导师, 1996年、2001于中国科学技术大学分别获得学士、博士学位, 主要研究方向为荧光传感技术及光纤传感技术, 并涉猎压电式传感器件产品、半导体传感器件产品的开发。E-mail:zdw@ustc.edu" ]
  [ "史凯兴 (1990-), 男, 山东济宁人, 硕士研究生, 2013年于青岛滨海学院获得学士学位, 主要研究方向为多功能传感器的研发与应用。E-mail:553558501@qq.com" ]
- 基金信息：
  
  国家自然科学基金资助项目(61167007);国家自然科学基金资助项目(61665008);航空科学基金项目(2016ZD56006);江西省青年科学家培养计划基金资助项目(20153BCB23037)
- DOI：10.3788/OPE.20172503.0591
  中图分类号： O657.31
- 收稿日期：2016-10-26，
  
  录用日期：2016-12-13，
  
  纸质出版日期：2017-03-25
- 稿件说明：
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张巍巍, 史凯兴, 赵小兵, 等. 发光染料罗丹明B的荧光传感特性[J]. 光学精密工程, 2017,25(3):591-596.

Wei-wei ZHANG, Kai-xing SHI, Xiao-bing ZHAO, et al. Multi-functional fluorescent sensing properties of rhodamine B[J]. Optics and precision engineering, 2017, 25(3): 591-596.
张巍巍, 史凯兴, 赵小兵, 等. 发光染料罗丹明B的荧光传感特性[J]. 光学精密工程, 2017,25(3):591-596. DOI： 10.3788/OPE.20172503.0591.

Wei-wei ZHANG, Kai-xing SHI, Xiao-bing ZHAO, et al. Multi-functional fluorescent sensing properties of rhodamine B[J]. Optics and precision engineering, 2017, 25(3): 591-596. DOI： 10.3788/OPE.20172503.0591.

摘要

本文对有机染料罗丹明B（RhB）的荧光光谱受温度、应力以及pH值等物理或化学量的影响进行了研究。实验采用波长为405 nm的激光激发RhB/H610胶黏剂复合材料或RhB水溶液，得到其荧光发射谱，用谱峰频移及新型表征方法“谱带重心频移法”分析了环境参数对谱带位置的影响，并通过线性拟合得到了RhB荧光谱带重心λ

传感温度、压应力以及pH值的传感方程。结果表明，谱带重心频移法有效抑制了光强涨落的白噪声，能够显著提高传感的精度和稳定性。在测量范围中间值附近，重心法对温度、压应力以及pH值传感的相对灵敏度分别为0.026%/℃，0.09%/MPa和0.34%。由于RhB具有很高的发光效率和优异的生物相容性，因此基于RhB荧光的传感方法在生物体系的检测表征方面具有较高的应用价值。

Abstract

The influence of physical or chemical factors such as temperature

stress and pH value on the fluorescence spectrum of organic dye rhodamine B (RhB) was researched experimentally. An RhB/H610-resin composite and aqueous solutions of RhB were excited under a diode laser with a wavelength of 405 nm. Then

the fluorescence spectra were obtained. The peak shift and the newly proposed barycenter shift of the emission band were employed to characterize the effect of the environmental factors on the band location. The sensing functions of waveband barycenter λ

with temperature

stress and pH value were respectively fitted linearly. The result shows that the barycenter method can effectively eliminate the random noise which mainly originated from the fluctuation of fluorescence intensity and light detector. Hence a finer precision was achieved in comparison with the peak shift method. The relative sensitivities of the barycentric method to the temperature

compressive stress and pH value are 0.026%/℃

0.09%/MPa and 0.34% respectively. Taking the advantages of the high fluorescence efficiency and biological histocompatibility of RhB

the fluorescent sensing with RhB is a promising candidate for characterization of biological samples. Particularly

it is suitable for applications in the real-time monitoring of microwave treatment.

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