利用光压差分技术筛选细胞的影响参数

程景萌; 杨丽; 周围; 李新冉; 张思祥

doi:10.3788/OPE.20172508.2029

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利用光压差分技术筛选细胞的影响参数

现代应用光学 | 更新时间：2020-07-07

- 利用光压差分技术筛选细胞的影响参数
- Analysis on influence parameters of cell sorting using optical pressure difference technology
- 光学精密工程 2017年25卷第8期页码：2029-2037
- 作者机构：
  
  河北工业大学机械工程学院, 天津 300130
- 作者简介：
  
  [ "程景萌(1981-), 女, 河北石家庄人, 实验师, 博士研究生, 2005年、2011年于河北工业大学分别获得学士、硕士学位, 主要从事微流控芯片与细胞驱动的研究。E-mail:81745535@qq.com" ]
  张思祥(1959-), 男, 天津人, 教授, 博士生导师, 1982年、1993年于浙江大学分别获得学士、硕士学位, 1996于天津大学获得博士学位, 主要从事光学仪器和分析仪器的研究。E-mail:zhangsixiang@hebut.edu.cn ZHANG Si-xiang, E-mail:zhangsixiang@hebut.edu.cn
- 基金信息：
  
  河北省青年科学基金资助项目(B2014202260)
- DOI：10.3788/OPE.20172508.2029
  中图分类号： TH79;O657.3
- 收稿日期：2017-03-13，
  
  录用日期：2017-4-14，
  
  纸质出版日期：2017-08-25
- 稿件说明：
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程景萌, 杨丽, 周围, 等. 利用光压差分技术筛选细胞的影响参数[J]. 光学精密工程, 2017,25(8):2029-2037.

Jing-meng CHENG, Li YANG, Wei ZHOU, et al. Analysis on influence parameters of cell sorting using optical pressure difference technology[J]. Optics and precision engineering, 2017, 25(8): 2029-2037.
程景萌, 杨丽, 周围, 等. 利用光压差分技术筛选细胞的影响参数[J]. 光学精密工程, 2017,25(8):2029-2037. DOI： 10.3788/OPE.20172508.2029.

Jing-meng CHENG, Li YANG, Wei ZHOU, et al. Analysis on influence parameters of cell sorting using optical pressure difference technology[J]. Optics and precision engineering, 2017, 25(8): 2029-2037. DOI： 10.3788/OPE.20172508.2029.

摘要

本文采用有限体积法建立了交叉型细胞分离模型，提出了一种基于光压差分的细胞筛选仿真方法，分析微流体中细胞筛选的影响因素。基于层流、流体流动粒子追踪、波动光学理论，利用有限元分析法建立了一种交叉型光学颗粒分离模型，研究了利用光压差分技术分离细胞的各种影响因素，其中包括微粒直径，激光功率、温度、光纤直径，分析了微粒在流体中因光辐射压力作用下的偏移距离。实验结果表明：在微流体中，激光功率、细胞直径、温度（20℃）和偏移距离大体上成正比关系，光纤直径和细胞直径在大小相当的情况下光辐射压力能够达到最大值，当激光通过光纤作用于直径分别为3，8和20

m的微粒时，光纤直径为7

m或8

m时光辐射压力最大，所以选用直径为8

m的单模光纤作为一个重要的实验光学器件。所得结论为深入研究细胞筛选影响因素的数值仿真精度提供了参考与借鉴。

Abstract

In this paper

the finite volume method was adopted to establish cross cell separation model

and a cell screening simulation method was proposed based on light pressure difference for analysis of influence factors of cell screening in microfluidics. Based on laminar flow

fluid flow

particle tracking and wave optics theories

a cross type optical particle separation model was established by finite element analysis. Using light pressure difference technology

various factors affecting the separation of cells were studied

including particle diameter

laser power

temperature and fiber diameter. Furthermore

deflection distances of the particles in the fluid due to the optical radiation pressure were determined experimentally. The results show that in microfluidics

laser power

cell diameter and temperature (20℃) generally are proportional to deflection distance separately. When fiber diameter and cell diameter are in the same size

optical radiation pressure reaches the maximum. When laser acts on particles with diameter of 3

m and 20

m through fibers with diameters of 7

m or 8

radiation pressure reaches its maximum

thus choosing single mode fiber with diameter of 8

m as an important optical device in the experiments. The above conclusion provides an reference for further study of precision of numerical simulation on cell screening factors.

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Keywords

references

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