花椒中罗丹明B的激光拉曼现场检测

张亮亮; 杨练根; 何涛; 范志勇

doi:10.3788/OPE.20172513.0026

您当前的位置：

首页 >

文章列表页 >

花椒中罗丹明B的激光拉曼现场检测

现代应用光学 | 更新时间：2020-08-13

- 花椒中罗丹明B的激光拉曼现场检测
- Field inspection of rhodamine B in prickly ash by laser Raman
- 光学精密工程 2017年25卷第10s期页码：26-31
- 作者机构：
  
  1. 湖北工业大学机械工程学院,湖北武汉,430068
  2. 湖北省食品安全质量监督检验研究院,湖北武汉,430075
- 作者简介：
- 基金信息：
  
  湖北省科技支撑计划资助项目（No.2015BCE047）()
- DOI：10.3788/OPE.20172513.0026
  中图分类号： TH742
- 收稿日期：2017-05-27，
  
  修回日期：2017-06-18，
  
  纸质出版日期：2017-11-25
- 稿件说明：
移动端阅览
张亮亮, 杨练根, 何涛等. 花椒中罗丹明B的激光拉曼现场检测[J]. 光学精密工程, 2017,25(10s): 26-31

ZHANG Liang-liang, YANG Lian-gen, HE Tao etc. Field inspection of rhodamine B in prickly ash by laser Raman[J]. Editorial Office of Optics and Precision Engineering, 2017,25(10s): 26-31
张亮亮, 杨练根, 何涛等. 花椒中罗丹明B的激光拉曼现场检测[J]. 光学精密工程, 2017,25(10s): 26-31 DOI： 10.3788/OPE.20172513.0026.

ZHANG Liang-liang, YANG Lian-gen, HE Tao etc. Field inspection of rhodamine B in prickly ash by laser Raman[J]. Editorial Office of Optics and Precision Engineering, 2017,25(10s): 26-31 DOI： 10.3788/OPE.20172513.0026.

摘要

鉴于实验室检测非法食品添加物存在设备昂贵、检测效率低及样品制备复杂等问题，本文设计了一种基于激光拉曼原理、用于测定花椒中罗丹明B的现场检测方法。它主要由激光器、光谱仪、激光探头、真空泵、分析软件以及便携式PC组成。利用拉曼原理设计了便携式光谱仪，并设计了分段线性拟合法和缺点多项式拟合法两种去基线算法以及结果匹配算法。对其进行对样品进行预处理，配合银离子拉曼增强剂，获取相应的拉曼光谱信号并进行结果匹配。实验结果表明，这种方法可以准确检测含有1×10

-6

罗丹明B的花椒样品，整个检测时间小于10 min，基本满足对调味品中非法添加物的现场快速检测要求。

Abstract

Prickly ash

dry pepper

chili sauce and other condiment are widely used in food industry and daily life. However these condiments are illegally added with rhodamine B

alkaline orange Ⅱ and other harzardous additives

which seriously affect human health. In order to inspect these illegal additives with low-cost and high-efficiency

a field detection system for rhodamine B in prickly ash was designed based on the principle of laser Raman. It mainly consisted of laser

spectrometer

laser probe

vacuum pump

analysis software and portable PC. A portable spectrometer based on Raman principle was achieved. Moreover

two baseline removal algorithms including piecewise linear fitting method as well as fault polynomial fitting method and a result matching algorithm were proposed. The samples were pretreated with silver ion Raman enhancer to obtain the corresponding Raman signals

which were used to match the results. The experiments prove that this method can accurately detect 1×10

-6

rhodamine B in prickly ash sample with whole inspection time less than 10 min

which can basically meet the requirements of field rapid inspection of illegal additives in condiments.

关键词

Keywords

references

黄亚伟.张令.王若兰,等. 表面增强拉曼光谱在食品非法添加物检测中的应用进展[J]. 粮食与饲料工业,2014,9(7):24-27. HUANG Y W, ZHANG L, WANG R L. Progress in application of surface enhanced Raman spectroscopy to the detection of illicit food additives[J]. Food and Feed Industry, 2014, 9(7):24-27. (in Chinese)

LAI K Q,ZHANG Y Y, LI CH Y. Analyses of illegal food additives with surface enhanced raman spectroscopy[J]. Optics Letters,2013,34(8):161-163.

刘敏. 高效液相色谱法测定食品中的添加剂和非法添加物[D].泰安:山东农业大学,2012. LIU M. Determination of Food Addictives and Hlegal Addictives by HPLC in Food[D]. Taian:Shandong Agricultural University, 2012. (in Chinese)

胡侠. 食品中罗丹明类染料的高效液相的色谱及高效液相色谱串联质谱分析方法的研究[D].大连:大连理工大学,2010 HU X. Study on the Analytical Methods of Rhodamine Dyes by HPLC and HPLC-MS/MS[D]. Dalian:Dalian University of Technology, 2010. (in Chinese)

林清荷.陈鑫.吴忠兴. 辣椒粉中罗丹明B液相色谱检测方法的研究[J]. 农产品加工学刊,2012,11(2):157-164. LIN Q H, CHEN X,WU ZH X.HPLC determination of Rhodamine B in chilli powder[J]. Journal of Agricultural Products Processing, 2012, 11(2):157-164. (in Chinese)

王传现.韩丽.方晓明,等.食品中罗丹明B的高效液相色谱荧光检测[J]. 分析仪器, 2008,1(2):27-30. WANG CH X,HAN L,FANG X M.Determination of Rhodamine B in food by high performance liquid chromatography-fluorescence detection[J]. Analytical Instruments, 2008, 1(2):27-30. (in Chinese)

程梁. 微型光谱仪系统的研究及其应用[D].杭州:浙江大学, 2008. CHENG L. Research and Application of Micro Spectrometer System[D]. Hangzhou:Zhejiang University, 2008. (in Chinese)

张溱, 杨练根, 张娜,等. 用于调味品中非法添加物测定的便携式激光拉曼检测仪[J]. 光学精密工程, 2016, 24(10s):203-211. ZHANG ZH, YANG L G, ZHANG N, et al.. Portable laser Raman detector for determination of illegal additives in condiments[J]. Opt. Precision Eng., 2016, 24(10s):203-211. (in Chinese)

SOYLAK M, UNSAL Y E, YILMAZ E, et al.. Determination of rhodamine B in soft drink, waste water and lipstick samples after solid phase extraction[J]. Food & Chemical Toxicology, 2011, 49(8):1796-1799.

JAHN M, PATZE S, BOCKLITZ T.Towards SERS based applications in food analytics:Lipophilic sensor layers for the detection of Sudan Ⅲ in food matrices[J]. Analytica Chimica Acta, 2015,860(20):43-50.

GAO J Z, ZENG L J, REN J. Determination of rhodamine B in capsicum powder by using a modified belousov-zhabotinskii oscillating chemical reaction[J]. Journal of Hunan Agricultural University, 2013, 39(3):327-330.

郭忠. 微型拉曼光谱仪的结构设计与数据处理的方法研究[D].重庆:重庆大学,2010. GUO ZH. Design the Micro-Raman Spectroscopy and Study on Data Processing of Raman Spectrum[D]. Chongqing:Chongqing University, 2010. (in Chinese)

浏览量

483

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据