便携式MicroRNA快速检测系统

王燕飞; 余东升; 陈海燕; 张战盈; 方蔚恺; 杨喆; 陆泽橼; 李艳蕾; 纪玉峰; 关一夫; 徐赤东

doi:10.3788/OPE.20182603.0541

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便携式MicroRNA快速检测系统

现代应用光学 | 更新时间：2020-07-05

- 便携式MicroRNA快速检测系统
- Portable rapid microRNA detection system
- 光学精密工程 2018年26卷第3期页码：541-547
- 作者机构：
  
  1.中国科学院合肥物质科学研究院医学物理与技术中心, 安徽合肥 230031
  2.中国科学技术大学, 安徽合肥 230026
  3.医学物理与技术安徽省重点实验室, 安徽合肥 230031
  4.中国医科大学, 辽宁沈阳 110001
- 作者简介：
  
  [ "王燕飞(1984-), 男, 安徽肥东人, 博士研究生, 2006年于合肥工业大学获得学士学位, 主要从事光电检测方面的研究。E-mail:yfw@mail.ustc.edu.cn" ]
  [ "徐赤东(1978-), 男, 安徽桐城人, 副研究员, 2007年于西安电子科技大学获得硕士学位, 主要研究方向为微脉冲激光雷达系统研制和激光雷达在大气探测中的应用。E-mail:xcd@aiofm.ac.cn" ]
- 基金信息：
  
  国家自然科学基金资助项目(81371896)
- DOI：10.3788/OPE.20182603.0541
  中图分类号： X835
- 收稿日期：2017-07-10，
  
  录用日期：2017-9-10，
  
  纸质出版日期：2018-03-25
- 稿件说明：
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王燕飞, 余东升, 陈海燕, 等. 便携式MicroRNA快速检测系统[J]. 光学精密工程, 2018,26(3):541-547.

Yan-fei WANG, Dong-sheng YU, Hai-yan CHEN, et al. Portable rapid microRNA detection system[J]. Optics and precision engineering, 2018, 26(3): 541-547.
王燕飞, 余东升, 陈海燕, 等. 便携式MicroRNA快速检测系统[J]. 光学精密工程, 2018,26(3):541-547. DOI： 10.3788/OPE.20182603.0541.

Yan-fei WANG, Dong-sheng YU, Hai-yan CHEN, et al. Portable rapid microRNA detection system[J]. Optics and precision engineering, 2018, 26(3): 541-547. DOI： 10.3788/OPE.20182603.0541.

摘要

MicroRNA（miRNA）与疾病的发生和发展密切相关，可作为疾病诊断标志物。常规检测miRNA的方法耗时较长且操作复杂，本文设计了一种便携式miRNA快速检测系统。该系统采用光电检测技术检测滚环扩增后的标志物miRNA受激发出的荧光强度，对采集到的荧光数据进行分析，得出miRNA的变化情况。实验得出便携式miRNA快速检测系统在试剂浓度为0.1~1

mol时，荧光强度线性偏倚不超过±5%，系统重复性大于95%。通过对冠心病检测标志物miR-499进行滚环扩增实验，结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA。

Abstract

MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker. Conventional methods for the detection of miRNA are time-consuming and complex. Herein

a portable rapid miRNA detection system was designed. This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique

and analyzes the fluorescence data to obtain the changes in miRNA. Through our experiments

we observed that for reagent concentrations in the range 0.1-1

mol

the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5%

and the repeatability was ≥ 95%. Our roll amplification experiment involving the coronary heart disease diagnostic marker

miR-499

confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.

关键词

Keywords

references

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