应用ISODATA选取可行域的荧光分子断层成像

侯榆青; 胡跃林; 易黄建; 焦璞; 贺小伟

doi:10.3788/OPE.20182608.2074

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应用ISODATA选取可行域的荧光分子断层成像

信息科学 | 更新时间：2020-08-13

- 应用ISODATA选取可行域的荧光分子断层成像
- Fluorescence molecular tomography of permissible region selected by ISODATA
- 光学精密工程 2018年26卷第8期页码：2074-2083
- 作者机构：
  
  西北大学信息科学与技术学院西安市影像组学与智肾感知重点实验室, 陕西西安 710127
- 作者简介：
  
  [ "侯榆青(1963-), 女, 陕西榆林人, 教授, 博士生导师, 1984年于西北大学获得学士学位, 1990年于中国科学院西安光机所获得硕士学位, 主要从事数字图像处理、医疗大数据相关影像组学研究。E-mail:houyuqin@nwu.edu.cn" ]
  [ "胡跃林(1993-), 男, 山西大同人, 硕士研究生, 2016年于陕西科技大学获得学士学位, 2016至今攻读西北大学硕士学位, 主要从事荧光分子断层成像的研究。E-mail:hun9981@sina.cn" ]
- 基金信息：
  
  国家自然科学基金资助项目(61372046);国家自然科学基金资助项目(11571012);国家自然科学基金资助项目(61640418);陕西省教育厅服务地方专项计划资助项目(17JF027);中国博士后科学基金资助项目(2016M602851);陕西省自然科学基础研究计划资助项目(2018JQ6099);西安市科技创新计划资助项目(201805060ZD11CG44)
- DOI：10.3788/OPE.20182608.2074
  中图分类号： TP391;Q632
- 收稿日期：2017-11-16，
  
  录用日期：2018-1-16，
  
  纸质出版日期：2018-08-25
- 稿件说明：
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侯榆青, 胡跃林, 易黄建, 等. 应用ISODATA选取可行域的荧光分子断层成像[J]. 光学精密工程, 2018,26(8):2074-2083.

Yu-qing HOU, Yue-lin HU, Huang-jian YI, et al. Fluorescence molecular tomography of permissible region selected by ISODATA[J]. Optics and precision engineering, 2018, 26(8): 2074-2083.
侯榆青, 胡跃林, 易黄建, 等. 应用ISODATA选取可行域的荧光分子断层成像[J]. 光学精密工程, 2018,26(8):2074-2083. DOI： 10.3788/OPE.20182608.2074.

Yu-qing HOU, Yue-lin HU, Huang-jian YI, et al. Fluorescence molecular tomography of permissible region selected by ISODATA[J]. Optics and precision engineering, 2018, 26(8): 2074-2083. DOI： 10.3788/OPE.20182608.2074.

摘要

为了实现快速、准确、鲁棒的荧光分子断层成像（FMT）重建，有限投影FMT和可行域选取策略得到了越来越多的关注。为了解决现有的可行域选取方法中存在的参数设置困难以及多目标选取不准确的问题，从而提高有限投影FMT的重建质量，提出了应用迭代自组织数据分析技术算法（ISODATA）的FMT可行域选取方法。首先采用ISODATA对初级重建结果聚类分区，然后在各分离的区域上分别选取可行域。为了验证提出的方法在应用中的可行性和有效性，设计了三目标荧光团重建的对比实验。实验结果显示，使用2个投影数据时，只有使用本文提出的方法可以准确地重建出三个荧光源的位置；使用4个投影数据时，重建的平均位置误差为0.18 mm，荧光产额相对误差小于50%，而此时使用阈值法不能重建，使用区域收缩法的荧光产额相对误差为61.2%。即使在测量数据较少时，提出的方法也可以准确高效地选取可行域，提高有限投影FMT重建的精确度和鲁棒性。

Abstract

In order to achieve a fast

accurate

and robust reconstruction of fluorescence molecular tomography (FMT)

limited-projection FMT and permissible region selection methods have been drawing more and more attention. Aiming to solve the problems of existing permissible region selection methods including the difficulty of parameter setting and the inaccuracy of multi-objective selection

so as to improve reconstruction quality of the limited-projection FMT

a method through which permissible regions were selected by applying an iterative self-organizing data analysis technique algorithm (ISODATA) was proposed. Firstly

ISODATA was used to cluster the primary reconstruction results

and then permissible regions were selected at every separated cluster. A contrast experiment of reconstructing three target fluorophores was designed to verify the feasibility and effectiveness in the application. The experimental results indicate that positions of all the three fluorophores can be reconstructed accurately with two projections only by using the proposed selection method. With four projections

the average localization error of reconstruction results is 0.18 mm and the relative error of the fluorescence yield is less than 50%. Meanwhile

the threshold method fails to reconstruct and the relative error of the fluorescence yield using the region-shrinking method is 61.2%. The proposed method is able to select permissible regions accurately and efficiently even with little measurement data. Consequently

the accuracy and robustness of limited-projection FMT reconstruction are improved.

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Keywords

references

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