薄膜Lamb波传感器用于胃蛋白酶原Ⅰ检测

魏巍; 张威; 孔慧; 李传宇; 张芷齐; 周连群

doi:10.3788/OPE.20182609.2280

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薄膜Lamb波传感器用于胃蛋白酶原Ⅰ检测

更新时间：2020-08-13

- 薄膜Lamb波传感器用于胃蛋白酶原Ⅰ检测
- Thin film Lamb wave sensors for pepsinogen Ⅰ detection
- 光学精密工程 2018年26卷第9期页码：2280-2288
- 作者机构：
  
  1.中国科学院苏州生物医学工程技术研究所中国科学院生物医学检验技术重点实验室, 江苏苏州 215163
  2.中国科学院大学材料科学与光电技术学院, 北京 100049
  3.中国科学技术大学, 安徽合肥 230026
- 作者简介：
  
  [ "魏巍(1993-), 男, 陕西渭南人, 硕士研究生, 2015年于北京化工大学获得学士学位, 主要从事生物传感器设计制作及生物医学应用的研究。E-mail:wei.wei01@hotmail.com" ]
  周连群(1981-), 男, 山东金乡人, 中法双博士, 研究员, 博士生导师, 主要从事微纳生物传感器及系统的研究。E-mail:zhoulq@sibet.ac.cnZHOU Lian-qun, E-mail:zhoulq@sibet.ac.cn
- 基金信息：
  
  国家自然科学基金资助项目(51675517);江苏省优秀青年基金资助项目(BO2015007);中国科学院青年创新促进会资助项目(2014280);中国科学院青年创新促进会资助项目(2018360);江苏省重大产业项目(BO2015007);苏州市民生科技项目(SS201749);院装备项目(YZ201638)
- DOI：10.3788/OPE.20182609.2280
  中图分类号： TP394.1;TH691.9
- 收稿日期：2018-01-26，
  
  录用日期：2018-3-30，
  
  纸质出版日期：2018-09-25
- 稿件说明：
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魏巍, 张威, 孔慧, 等. 薄膜Lamb波传感器用于胃蛋白酶原Ⅰ检测[J]. 光学精密工程, 2018,26(9):2280-2288.

Wei WEI, Wei ZHANG, Hui KONG, et al. Thin film Lamb wave sensors for pepsinogen Ⅰ detection[J]. Optics and precision engineering, 2018, 26(9): 2280-2288.
魏巍, 张威, 孔慧, 等. 薄膜Lamb波传感器用于胃蛋白酶原Ⅰ检测[J]. 光学精密工程, 2018,26(9):2280-2288. DOI： 10.3788/OPE.20182609.2280.

Wei WEI, Wei ZHANG, Hui KONG, et al. Thin film Lamb wave sensors for pepsinogen Ⅰ detection[J]. Optics and precision engineering, 2018, 26(9): 2280-2288. DOI： 10.3788/OPE.20182609.2280.

摘要

为满足大规模胃癌早期筛查对胃蛋白酶原Ⅰ（PGI）检测高灵敏度、高效率、操作简单、样品量少的需求，本文构建了一种PGI抗体功能化薄膜型Lamb波生物传感器。对传感器检测腔薄膜进行PGI抗体自组装修饰，传感器检测腔表面修饰的PGI抗体将样品中PGI抗原特异性的捕获并固定在检测腔薄膜表面，Lamb波传感器薄膜表面质量增加导致其A

模式中心频率发生移动，且频率移动量与检测腔表面吸附物质质量增加量正相关，实现对样本中PGI抗原浓度的检测。实验结果表明：PGI抗体功能化薄膜Lamb波生物传感器对PGI抗原实测灵敏度约为102.114 Hz/ng/mL，理论最低检测限（LOD）为0.176 ng/mL，单个样本检测时间为40 min，与现有基于光学检测法PGI检测技术相比，具有检测系统简单、操作简单、不需要专业人员操作等显著优势，且比多数光学检测法LOD更低，比电化学法PGI检测技术LOD低两个数量级。结果表明，本文提出的PGI抗体免疫功能化薄膜型Lamb波生物传感器对PGI检测且具有检测下限低、灵敏度高、检测效率高、操作简单、无需样品预处理等特点，满足大规模早期胃癌筛查的基本需求。

Abstract

To achieve the requirements of high sensitivity

high efficiency

simple operation

and low sample size for the detection of pepsinogen Ⅰ (PGI) in large-scale early screening of gastric cancer

a thin-film Lamb wave device with PGI antibody functionality was constructed in this study. The thin film in the device detection cavity was modified with self-assembly of the PGI antibody

which specifically captured and immobilized the PGI antigen in the samples on the surface of the thin film. The resultant increase of mass on the surface led to change of the A

mode center frequency

and the variation was positively correlated with the increment of mass absorbed on the chamber surface. Thus

the PGI antigen concentration in the samples was detected. The results showed that

when measuring the level of the PGI antigen

the thin-film resonance Lamb wave biosensor with PGI antibody functionality had a sensitivity of about 102.114 Hz/lg (ng/mL)

theoretical limit of detection (LOD) of 0.176 ng/mL

and detection time for one sample of 40 min. Compared with the existing PGI detection technology based on optical detection

the Lamb wave sensor has the advantages of being a simple detection system with simple operation and no need for professional operators

and lower LOD than most optical detection methods

two orders lower than that of the electrochemical method of PGI detection. In conclusion

the thin-film resonance Lamb wave biosensor with PGI antibody functionality proposed in this paper has the advantages of low LOD

high sensitivity

high detection efficiency

and simple operation

which can satisfy the basic requirements of large-scale early gastric cancer screening.

关键词

Keywords

references

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