用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统

丰斌; 姚佳; 张威; 孔慧; 魏巍; 周连群

doi:10.3788/OPE.20182609.2304

您当前的位置：

首页 >

文章列表页 >

用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统

更新时间：2020-08-13

- 用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统
- Parylene-C enhanced quartz crystal microbalance based dissipation detecting system for aPTT measurement
- 光学精密工程 2018年26卷第9期页码：2304-2311
- 作者机构：
  
  1.上海大学通信与信息工程学院, 上海 200444
  2.中国科学院苏州生物医学工程技术研究所, 江苏苏州 215163
  3.苏州大学电子信息学院, 江苏苏州 215006
- 作者简介：
  
  [ "丰斌(1991-), 男, 山东青岛人, 2014年于山东大学获得学士学位, 2015年攻读中国科学院苏州生物医学工程技术研究所与上海大学联合培养硕士研究生, 主要从事QCM传感器设计、测量系统设计与凝血应用研究。E-mail:fengbincn@foxmail.com" ]
  周连群(1981-), 男, 山东金乡人, 研究员, 博士生导师, 2010年于法国Université de Franche-Comté大学获得科学工程专业博士学位和中科院研究生院工学博士学位, 主要从事微纳生物传感器及系统方面的研究。E-mail:zhoulq@sibet.ac.cnZHOU Lian-qun, E-mail:zhoulq@sibet.ac.cn
- 基金信息：
  
  国家重点研发计划资助项目(2018YFC0115705);国家自然科学基金资助项目(51675517);江苏省优秀青年基金资助项目(BK20160057);中国科学院青年创新促进会资助项目(2014280);中国科学院青年创新促进会资助项目(2018360);江苏省优秀青年基金资助项目(BO2015007);苏州市民生科技项目资助(S201749);院装备项目(YZ201638)
- DOI：10.3788/OPE.20182609.2304
  中图分类号： TP394.1;TH691.9
- 收稿日期：2018-02-05，
  
  录用日期：2018-4-2，
  
  纸质出版日期：2018-09-25
- 稿件说明：
移动端阅览
丰斌, 姚佳, 张威, 等. 用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统[J]. 光学精密工程, 2018,26(9):2304-2311.

Bin FENG, Jia YAO, Wei ZHANG, et al. Parylene-C enhanced quartz crystal microbalance based dissipation detecting system for aPTT measurement[J]. Optics and precision engineering, 2018, 26(9): 2304-2311.
丰斌, 姚佳, 张威, 等. 用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统[J]. 光学精密工程, 2018,26(9):2304-2311. DOI： 10.3788/OPE.20182609.2304.

Bin FENG, Jia YAO, Wei ZHANG, et al. Parylene-C enhanced quartz crystal microbalance based dissipation detecting system for aPTT measurement[J]. Optics and precision engineering, 2018, 26(9): 2304-2311. DOI： 10.3788/OPE.20182609.2304.

摘要

房颤、血栓等患者抗凝药物日常口服等场景迫切需要凝血参数快速检测，本文设计和制作了一种Parylene-C增强型石英晶体微天平（QCM）传感器及其耗散因子检测系统用于凝血测量。首先使用Parylene-C有效增加石英晶体微天平传感器的峰峰值和有效使用次数，基于传感器耗散因子对血液凝固过程血液粘弹性变化敏感，设计电导谱分析法的压电传感器耗散因子快速测量系统，对血浆部分凝血活酶时间（aPTT）进行测量。并用SYSMEX CS 5100光学凝血仪、Lambda 950分光光度计验证系统测量结果。实验表明，Parylene-C增强型QCM传感器信号峰峰值增加8±1%，传感器aPTT实验有效重复使用次数为30次，系统30℃温差最大耗散偏移2.09×10

-6

。aPTT耗散曲线与光学法（lambda 950）吸光度曲线变化趋势一致。与SYSMEX CS 5100临床结果线性拟合决定系数

为0.99。同样本10次重复实验结果变异系数为1.48%。Parylene-C增强型QCM传感器与耗散分析法的联合应用具备多场景下凝血参数快速检测的能力，系统温度稳定性好，具有满足即时检测应用的潜力。

Abstract

To address the need to rapidly detect blood coagulation in daily administration of anticoagulant for atrial fibrillation and thrombus patients and others

a Parylene-C enhanced quartz crystal microbalance (QCM) sensor and a dissipation detection system were designed and manufactured for the measurement of coagulation parameters. Parylene-C was used to enhance the peak-to-peak value and reusability of the QCM sensor. A dissipation detection system for piezoelectric sensors was designed using the principle of conductance spectrum analysis to measure the activated partial thromboplastin time (aPTT) based on the sensitivity of the sensor's dissipation to the change of blood viscoelasticity in the coagulation process. The SYSMEX CS 5100 optical coagulometer system and Lambda 950 spectrophotometer were used to evaluate the system. The results indicate that Parylene-C increases the sensor's peak-to-peak value by 8±1% and it can be reused 30 times. The system has a maximum dissipation excursion of 2.09×10

-6

with a temperature difference of 30℃. The dissipation and absorbance curves of optical detection (Lambda 950) have the same variation trend. The adjusted R-square value of the linear fitting with SYSMEX CS 5100 is 0.99. The results of the experiment

repeated 10 times with the same sample

have a variable coefficient of 1.48%. The combination of the Parylene-C enhanced QCM and dissipation detection system is highly temperature-stable

can rapidly detect blood coagulation in different scenarios

and has the potential for point-of-care testing.

关键词

Keywords

references

CARDENAS J, REIN S C, CHURCH F. Overview of Blood Coagulation and the Pathophysiology of Blood Coagulation Disorders[M]. Amsterdam:Elsevier Press, 2016.

HARRIS L F, CASTRO L V, KILLARD A J. Coagulation monitoring devices:Past, present, and future at the point of care[J]. Trends in Analytical Chemistry, 2013, 50:85-95.

HARRIS L, LAKSHMANAN R S, EFREMOV V, et al.. Point of care (POC) blood coagulation monitoring technologies[M]. Medical Biosensors for Point of Care ( POC ) Applications . 2017.

HUSSAIN M, WENDEL H P, SCHMIDT K, et al.. QCM-D surpassing clinical standard for the dose administration of new oral anticoagulant in the patient of coagulation disorders[J]. Biosensors & Bioelectronics, 2017, 104:15.

WITT V, PICHLER H, BEIGLBOECK E, et al.. Changes in hemostasis caused by different replacement fluids and outcome in therapeutic plasma exchange in pediatric patients in a retrospective single center study[J]. Transfusion & Apheresis Science Official Journal of the World Apheresis Association Official Journal of the European Society for Haemapheresis, 2017, 56(1):59.

HUSSAIN M. QCM-D for Haemostasis Assays:Current Status and Future:A Review[J]. UK Journal of Pharmaceutical and Biosciences, 2016, 4(1):121-132.

LEI K F, CHEN K H, TSUI P H, et al.. Real-time electrical impedimetric monitoring of blood coagulation process under temperature and hematocrit variations conducted in a microfluidic chip[J]. Plos One, 2013, 8(10):e76243.

CHEN D, SONG S, MA J, et al.. Micro-electromechanical film bulk acoustic sensor for plasma and whole blood coagulation monitoring[J]. Biosensors & Bioelectronics, 2016, 91:465.

YANG C L, HUANG S J, CHOU C W, et al.. Design and evaluation of a portable optical-based biosensor for testing whole blood prothrombin time[J]. Talanta, 2013, 116(22):704-711.

薛严冰, 李亚, 于婧怡.基于PANI/SnO 2 符合材料的QCM水果气体传感器[J].光学精密工程, 2015, 24(10):0511-0519.

XUE Y B, LI Y, YU J Y. QCM fruit gas sensor based on PANI/SnO 2 composite materials[J]. Opt. Precision Eng., 2015, 24(10):0511-0519. (in Chinese)

张冬至.静电诱导自组装碳纳米管薄膜的结构表征与电学性能[J].光学精密工程, 2014, 22(6):1562-1570.

ZHANG D ZH. Structure characterization and electric properties of electrostatic-induced self-assembly carbon nanotube films[J]. Opt. Precision Eng., 2014, 22(6):1562-1570. (in Chinese)

HUSSAIN M, NORTHOFF H, GEHRING F K. QCM-D providing new horizon in the domain of sensitivity range and information for haemostasis of human plasma[J]. Biosensors and Bioelectronics, 2015, 66:579-584.

JIN J, JIANG W, YIN J, et al.. Stagnaro P. Plasma Proteins Adsorption Mechanism on Polyethylene-Grafted Poly(ethylene glycol) Surface by Quartz Crystal Microbalance with Dissipation[J]. Langmuir., 2013, 29(22):6624-6633.

EFREMOV V, KILLARD A J, BYRNE B, et al.. The modelling of blood coagulation using the quartz crystal microbalance[J]. Journal of Biomechanics, 2013, 46(3):437.

MULLER L, SINN S, DRECHSEL H, et al.. Investigation of prothrombin time in human whole-blood samples with a quartz crystal biosensor[J]. Analytical Chemistry, 2010, 82(2):658.

李敬, 潘海曦, 郭振, 等. Parylene增强型声表面波传感器及其温度响应[J].光学精密工程, 2017, 25(12):3048-3055.

LI J, PAN H X, GUO ZH, et al.. Parylene-enhanced SAW sensor and its temperature response[J]. Opt. Precision Eng., 2017, 25(12):3048-3055. (in Chinese)

YANG Y, WEI Z, ZHEN G, et al.. Stability Enhanced, Repeatability Improved Parylene-C Passivated on QCM Sensor for aPTT Measurement[J]. Biosensors and Bioelectronics, 2017, 98:41-46.

LAKSHMANAN R S, EFREMOV V, CULLEN S M, et al.. Measurement of the evolution of rigid and viscoelastic mass contributions from fibrin network formation during plasma coagulation using quartz crystal microbalance[J]. Sensors and Actuators B:Chemical, 2014, 192(3):23-28.

黄佳.高频小型石英晶体微天平的研究[D].东南大学, 2015.

HUANG J. Study on miniaturized high-frequency quzrtz crystal microbalance [D]. Southeast University, 2015. (in Chinese)

SINAURIDZE E I, VUIMO T A, TARANDOVSKIY I D, et al.. Thrombodynamics, a new global coagulation test:Measurement of heparin efficiency[J]. Talanta, 2017, 180:282-291.

浏览量

253

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

多壁碳纳米管增强型电化学传感器用于凝血酶原时间检测

活化部分凝血活酶时间检测石墨烯传感器研制

活化部分凝血活酶时间检测石墨烯传感器的研制

用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器