基于微流控和酶比色的微创血糖连续检测仪

徐可欣; 陈小龙; 栗大超; 于海霞

doi:10.3788/OPE.20182611.2615

您当前的位置：

首页 >

文章列表页 >

基于微流控和酶比色的微创血糖连续检测仪

现代应用光学 | 更新时间：2020-07-05

- 基于微流控和酶比色的微创血糖连续检测仪
- Minimally invasive continuous blood glucose monitor based on microfluidic and enzyme colorimetric technologies
- 光学精密工程 2018年26卷第11期页码：2615-2622
- 作者机构：
  
  天津大学精密仪器与光电子工程学院精密测试技术及仪器国家重点实验室, 天津 300072
- 作者简介：
  
  [ "徐可欣(1956-), 男, 安徽人, 博士, 教授, 博士生导师, 1985年、1988年于天津大学分别获得硕士、博士学位, 主要从事采用光谱方法进行人体微弱成分无创伤检测方法、生物光学及微弱化学成分智能化检测方法的研究。E-mail:kexin@tju.edu.cn" ]
  于海霞(1980-), 女, 河北人, 博士, 副研究员, 硕士生导师, 2007年、2011年于天津大学分别获得硕士、博士学位, 主要从事微创血糖检测方法的研究。E-mail:hxy2081@tju.edu.cnYU Hai-xia, E-mail:hxy2081@tju.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(81571766)
- DOI：10.3788/OPE.20182611.2615
  中图分类号： TH773;R318.6
- 收稿日期：2018-06-01，
  
  录用日期：2018-7-2，
  
  纸质出版日期：2018-11-25
- 稿件说明：
移动端阅览
徐可欣, 陈小龙, 栗大超, 等. 基于微流控和酶比色的微创血糖连续检测仪[J]. 光学精密工程, 2018,26(11):2615-2622.

Ke-xin XU, Xiao-long CHEN, Da-chao LI, et al. Minimally invasive continuous blood glucose monitor based on microfluidic and enzyme colorimetric technologies[J]. Optics and precision engineering, 2018, 26(11): 2615-2622.
徐可欣, 陈小龙, 栗大超, 等. 基于微流控和酶比色的微创血糖连续检测仪[J]. 光学精密工程, 2018,26(11):2615-2622. DOI： 10.3788/OPE.20182611.2615.

Ke-xin XU, Xiao-long CHEN, Da-chao LI, et al. Minimally invasive continuous blood glucose monitor based on microfluidic and enzyme colorimetric technologies[J]. Optics and precision engineering, 2018, 26(11): 2615-2622. DOI： 10.3788/OPE.20182611.2615.

摘要

连续血糖检测对糖尿病的诊断与治疗具有十分重要的意义。本文设计了一种集成化、自动化的微创血糖连续检测仪器，该仪器通过微流控芯片透皮抽取组织液，利用单片机精确测量透皮抽取组织液的体积，并采用酶比色法检测组织液的葡萄糖浓度，利用组织液与血液的葡萄糖浓度相关性实现连续血糖检测。由于透皮抽取的组织液体积很小且分散在皮肤表面，为了便于收集，利用生理盐水对抽取出的组织液进行稀释，稀释后的组织液中葡萄糖浓度在3~50 mg/dL。为了测量低浓度葡萄糖，实验选取了1~50 mg/dL中的10个浓度的葡萄糖溶液进行吸光度测量，根据光谱数据与葡萄糖浓度建立吸光度模型，结果表明该酶比色检测方法在1~50 mg/dL葡萄糖浓度内具有良好的线性度，测量相对标准偏差小于0.65%。该仪器能够实现自动化控制，为糖尿病的诊断提供依据，在微创血糖连续检测领域具有良好的应用前景。

Abstract

Continuous blood glucose detection is of great significance in the diagnosis and treatment of diabetes. In this study

an integrated

automated minimally invasive blood glucose continuous detection instrument was designed. Interstitial fluid (ISF) was transdermally extracted by a microfluidic chip

and the volume of the ISF was accurately measured by the single-chip computer. The glucose contained in the fluid was detected by enzyme colorimetry. Then

continuous blood glucose detection was achieved using the correlation of glucose concentration between the interstitial fluid and blood. The glucose concentration in the ISF was obtained by controlling the sample injection

reagent quantification

and absorbance detection in the enzyme colorimetric optical detection module. Owing to the small volume of the ISF extracted through the skin and dispersed on the surface of the skin

in order to facilitate collection

the extracted ISF was diluted with normal saline. To measure the low concentration of the diluted ISF

10 concentrations in the range of 1-50 mg/dL were selected for absorbance measurement. The absorbance model was established based on the spectral data and glucose concentration. The results show that the glucose detection method has good linearity in the range of 1-50 mg/dL

and the relative standard deviation is less than 0.65%. The instrument is an automation control system and can provide basis for the diagnosis of diabetes. The feasibility of the instrument is verified by test experiments. The instrument has good application prospects for continuous blood glucose monitoring.

关键词

Keywords

references

许曼音.糖尿病学[M].上海:上海科学技术出版社, 2004:189.

XU M Y. Diabetes Study [M]. Shanghai:Publishing of Shanghai Science and Technology, 2004:189.(in Chinese)

JANG S. Review of emerging approaches in non-or minimally invasive glucose monitoring and their application to physiological human body fluids[J]. International Journal of Biosensors & Bioelectronics , 2018, 4(1):87.

PANDEY R, PAIDI S K, VALDEZ T A, et al .. Noninvasive monitoring of blood glucose with Raman spectroscopy[J]. Accounts of Chemical Research , 2017, 50(2):264-272.

LITINSKAIA E L, BAZAEV N A, POZHAR K V, et al .. Methods for improving accuracy of non-invasive blood glucose detection via optical glucometer[J]. Young Researchers in Electrical & Electronic Engineering , 2017:47-49.

罗云瀚, 陈哲, 陈星旦.近红外光谱无创血糖测量的极限检测浓度[J].光学精密工程, 2008, 16(5):784-788.

LUO Y H, CHEN ZH, CHEN X D. Detection limit of glucose concentration in noninvasive sensing with near infrared spectroscopy[J]. Opt. Precision Eng. , 2008, 16(5):784-788.(in Chinese)

陈星旦, 高静, 丁海泉.论无创血糖监测的红外光谱方法(特邀)[J].中国光学, 2012(4):317-326.

CHEN X D, GAO J, DING H Q. Infrared spectroscopy for non-invasive blood glucose monitoring(Invited)[J]. Chinese Optics , 2012(4):317-326.(in Chinese)

LUO Y, CHEN X, XU M, et al .. Optofluidic glucose detection by capillary-based ring resonators[J]. Optics & Laser Technology , 2014, 56(1):12-14.

SHARMA S, TAKAGI E, CASS T, et al .. Minimally invasive microneedle array electrodes employing direct electron transfer type glucose dehydrogenase for the development of continuous glucose monitoring sensors[J]. Procedia Technology , 2017, 27:208-209.

WANG G, POSCENTE M D, PARK S S, et al .. Wearable microsystem for minimally invasive, pseudo-continuous blood glucose monitoring:the e-mosquito[J]. Transactions on Biomedical Circuits & Systems , 2017, 11(5):979-987.

栗大超, 李国卿, 张晶鑫, 等. GGBP蛋白修饰的表面等离子共振微创血糖检测仪[J].光学精密工程, 2013, 21(9):2333-2339.

LI D CH, LI G Q, ZHANG J X, et al .. Minimally-invasive blood glucose detection instrument based on surface plasmon resonance sensor decorated with GGBP[J]. Opt. Precision Eng. , 2013, 21(9):2333-2339.(in Chinese)

YU H, LI D, ROBERTS R C, et al .. An interstitial fluid transdermal extraction system for continuous glucose monitoring[J]. Journal of Microelectromechanical Systems , 2012, 21(4):917-925.

LI D CH, LU B Y, ZHU R, et al .. An optofluidic system with volume measurement and surface plasmon resonance sensor for continuous glucose monitoring[J]. Biomicrofluidics , 2016, 10(1):011913.

于海霞, 栗大超, 刘同坤, 等.基于微流体技术的组织液透皮抽取装置[J].光学精密工程, 2011, 19(3):651-656.

YU H X, LI D CH, LIU T K, et al .. Interstitial fluid transdermal extraction tool based on microfluidics technology[J]. Opt. Precision Eng. , 2011, 19(3):651-656.(in Chinese)

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

GGBP蛋白修饰的表面等离子共振微创血糖检测仪

基于微流体技术的组织液透皮抽取装置