用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器

李超; 郭振; 张威; 姚佳; 孔慧; 严茹红

doi:10.3788/OPE.20192706.1345

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用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器

生命科学仪器 | 更新时间：2020-08-13

- 用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器
- Multi-walled carbon nanotubes enhanced electrochemical sensor for prothrombin time detection
- 光学精密工程 2019年27卷第6期页码：1345-1353
- 作者机构：
  
  1.中国科学技术大学，安徽合肥 230026
  2.中国科学院苏州生物医学工程技术研究所中国科学院生物医学检验技术重点实验室，江苏苏州 215163
  3.苏州大学电子信息学院，江苏苏州 215006
  4.苏州科技城医院检验科，江苏苏州 215153
- 作者简介：
  
  [ "李超(1993-)，男，山西长治人，硕士研究生，2015年于电子科技大学大学获得学士学位，主要从事电化学生物传感器设计和凝血测量技术研究。E-mail：lishimin@mail.ustc.edu.cn" ]
- 基金信息：
  
  国家自然科学基金资助项目(51675517);国家自然科学基金资助项目(61874133);江苏省优秀青年基金资助项目(BK20160057);江苏省自然科学基金资助项目(BK20170428);中国科学院STS项目资助(KFJ-STS-SCYD-217);江苏省重点研究开发项目资助(BE2018080);中国博士后科学基金资助项目(2018T110552);中国博士后科学基金资助项目(2016M601890);江苏省博士后科研计划资助项目(1701010A);中国博士后科研基金资助项目(2018K071C);苏州市医疗工程计划资助项目(Y851431103);苏州市医疗工程计划资助项目(Y851391105);苏州市医疗工程计划资助项目(Y831191103);苏州市民生科技项目(SS201749);苏州新区医疗卫生科技计划资助项目(2016Q006)
- DOI：10.3788/OPE.20192706.1345
  中图分类号： TP212
- 收稿日期：2018-12-21，
  
  录用日期：2019-2-25，
  
  纸质出版日期：2019-06-15
- 稿件说明：
移动端阅览
李超, 郭振, 张威, 等. 用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器[J]. 光学精密工程, 2019,27(6):1345-1353.

Chao LI, Zhen GUO, Wei ZHANG, et al. Multi-walled carbon nanotubes enhanced electrochemical sensor for prothrombin time detection[J]. Optics and precision engineering, 2019, 27(6): 1345-1353.
李超, 郭振, 张威, 等. 用于凝血酶原时间检测的多壁碳纳米管增强型电化学传感器[J]. 光学精密工程, 2019,27(6):1345-1353. DOI： 10.3788/OPE.20192706.1345.

Chao LI, Zhen GUO, Wei ZHANG, et al. Multi-walled carbon nanotubes enhanced electrochemical sensor for prothrombin time detection[J]. Optics and precision engineering, 2019, 27(6): 1345-1353. DOI： 10.3788/OPE.20192706.1345.

摘要

针对目前凝血检测仪器存在的成本昂贵操作复杂等问题，制作了一种基于丝网印刷技术的多壁碳纳米管(MWCNTs)增强型电化学传感器用于凝血酶原时间(PT)测量。利用凝血酶切割凝血酶底物实验验证了计时电流法检测凝血酶原时间原理的可行性。接着对血浆凝血酶原时间参数进行测量，并用SYSMEX CS 5100光学凝血仪验证测量结果。经过测试，凝血酶验证性实验中MWCNT型电化学传感器的电流响应强度较普通电化学传感器增加了(36±1)%，重复实验出峰时间和峰值电流变异系数分别为2.99%和3.27%。测试不同血样PT值，能够清晰的显示出区分度，且挑选三组血样进行PT重复实验，出峰时间变异系数分别为2.26%、3.22%和2.96%，实验结果与医院的临床结果线性拟合决定系数

为0.986。MWCNTs增强型型电化学传感器用于PT测试具有良好的重复性和一致性，易于批量生产，大大降低了凝血测量的成本，且适合多种场合的测量，在即时检测领域具有极大潜力。

Abstract

The determination of coagulation parameters is of crucial significance in the guided treatment of anticoagulant drug users and patients with liver diseases. Considering the challenges associated with commercial coagulation detection instruments such as high cost and complex operation

a MWCNT-enhanced screen-printed electrochemical sensor was developed for coagulation measurement. Initially

the measurement of thrombin substrate cleaved by thrombin was used to evaluate the feasibility of prothrombin time (PT) detection by chronoamperometry. Then the plasma PT parameters were measured and the results were validated using the SYSMEX CS 5100 optical coagulator. The response intensity of a MWCNT electrochemical sensor in thrombin validation experiments increase by (36±1)% compared with a general electrochemical sensor and the peak time coefficient of variation and peak current coefficients of variation are 2.99% and 3.27% respectively. The testing of PT values of different blood samples clearly shows discrimination. Three groups of blood samples are selected for repeated PT parameter measurements and the coefficients of variation are 2.26%

3.22%

and 2.96% respectively. The linear fitting decision coefficient

is 0.986 for clinical results. The MWCNT screen-printed electrochemical sensor for PT testing has good repeatability and consistency

is easy to mass produce

reduces the cost of coagulation measurement

and is suitable for measurement in many circumstances. As such

this sensor has great potential in the field of point-of-care testing.

关键词

Keywords

references

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