光干涉生物亲和性检测系统设计

张硕; 嵇晓强; 常知强; 史健松; 李琦; 张昊; 李美萱

doi:10.3788/OPE.20192706.1378

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光干涉生物亲和性检测系统设计

生命科学仪器 | 更新时间：2020-08-13

- 光干涉生物亲和性检测系统设计
- Design of bioaffinity detection system based on optical interference
- 光学精密工程 2019年27卷第6期页码：1378-1386
- 作者机构：
  
  1.长春理工大学生命科学技术学院，吉林长春 130022
  2.吉林工程技术师范学院量子信息技术交叉学科研究院，吉林长春 130052
  3.吉林省量子信息技术工程实验室，吉林长春 130052
- 作者简介：
  
  [ "张硕(1994-)，男，河北唐山人，硕士研究生，主要从事光电医学仪器及生物检测技术研究。E-mail：2246382487@qq.com" ]
  嵇晓强(1982-)，女，吉林德惠人，博士，副教授，2012年于中科院长春光机所光学工程获得博士学位，主要从事光电医学仪器设计及图像处理。E-mail：zuoanmulan@163.com JI Xiao-qiang, E-mail:zuoanmulan@163.com
  李美萱(1984-)，女，吉林梅河口人，讲师，博士，2007年、2010年、2017年于长春理工大学分别获得学士、硕士、博士学位，主要从事多光谱关联成像、光谱学与光谱分析研究。E-mail：limx@jlenu.edu.cn LI Mei-xuan, E-mail:limx@jlenu.edu.cn
- 基金信息：
  
  吉林省科技发展计划资助项目(20170309003YY);吉林省教育厅计划资助项目(JJKH20181142KJ)
- DOI：10.3788/OPE.20192706.1378
  中图分类号： TH741
- 收稿日期：2019-04-16，
  
  录用日期：2019-4-25，
  
  纸质出版日期：2019-06-15
- 稿件说明：
移动端阅览
张硕, 嵇晓强, 常知强, 等. 光干涉生物亲和性检测系统设计[J]. 光学精密工程, 2019,27(6):1378-1386.

Shuo ZHANG, Xiao-qiang JI, Zhi-qiang CHANG, et al. Design of bioaffinity detection system based on optical interference[J]. Optics and precision engineering, 2019, 27(6): 1378-1386.
张硕, 嵇晓强, 常知强, 等. 光干涉生物亲和性检测系统设计[J]. 光学精密工程, 2019,27(6):1378-1386. DOI： 10.3788/OPE.20192706.1378.

Shuo ZHANG, Xiao-qiang JI, Zhi-qiang CHANG, et al. Design of bioaffinity detection system based on optical interference[J]. Optics and precision engineering, 2019, 27(6): 1378-1386. DOI： 10.3788/OPE.20192706.1378.

摘要

分子互作检测是目前食品安全检测、临床癌症病理筛查等检测领域的研究热点。为了对生物分子间相互作用进行实时、快速检测，本文以光干涉技术为理论基础，设计了基于光纤生物传感器的分子互作检测系统。采用了石英光纤与自聚焦透镜耦合结构，提高了光源与干涉光的传输光路耦合效率。采用STM32微处理器作为主控制器，实现了数据采集、三维传动机构控制、恒温震荡控制、通讯等功能。最后，采用所设计的分子互作检测系统及美国Fortebio公司的BLItz分子互作检测系统，分别进行不同浓度的定量检测实验，实时检测IgG与抗原分子蛋白A的结合与解离反应。实验结果表明：本文设计的系统检测极限达到10 μg/mL，重复性CV值小于6%，与BLItz接近。所设计系统检测的自动化程度高、检测过程中无需清洗、不产生交叉污染、成本低，能够满足药代动力学研究需要。

Abstract

Molecular interaction detection is a hot topic in the fields of food safety detection

clinical cancer pathological screening

and other areas of research. To realize real-time and rapid detection of interactions between biomolecules

a molecular interaction detection system based on a fiber biosensor was designed in this study. The coupling structure of a self-focusing lens and quartz fiber was adopted to improve the coupling efficiency of optical transmission between the light source and interference light. A STM32 microprocessor was used as the main controller to realize data acquisition

3D transmission mechanism control

constant temperature oscillation control

and other functions. Finally

the designed and BLItz molecular interaction detection systems were used to detect the different concentrations of immunoglobulin IgG and protein A

respectively. The binding and dissociation reactions of the IgG molecule and antigen molecule protein A were detected in real time. The experimental results show that the detection limit of the system could reach 10 μg/mL

and the repeatability CV value was less than 6%

which was close to that of the BLItz. The designed system has the advantages of high automation

no need for cleaning

no cross contamination in the detection process

and low cost. Therefore

it can meet the needs of pharmacokinetics research.

关键词

Keywords

references

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