种子呼吸CO<sub>2</sub>浓度检测系统

贾良权; 祁亨年; 胡文军; 赵光武; 阚瑞峰

doi:10.3788/OPE.20192706.1397

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种子呼吸CO₂浓度检测系统

生命科学仪器 | 更新时间：2020-08-13

- 种子呼吸CO₂浓度检测系统
- CO₂ concentration detection system for seed respiration
- 光学精密工程 2019年27卷第6期页码：1397-1404
- 作者机构：
  
  1.湖州师范学院，浙江湖州 313000
  2.浙江农林大学，浙江杭州 311000
  3.中国科学院长春光学精密机械与物理研究所应用光学国家重点实验室，吉林长春 130033
- 作者简介：
  
  [ "贾良权(1984-)男，安徽合肥人，博士后，硕士生导师，2006年获得安徽工程大学学士学位，2015年获得中国科学院大学博士学位，主要从事种子活力检测与农业光谱技术的研究。E-mail：02426@zjhu.edu.cn" ]
  阚瑞峰(1977-)，男，辽宁锦州人，研究员，博士生导师，2000年于中科院长春光学精密机械学院获得学士学位，2003年于中科院安徽光学精密机械研究所获得硕士学位，2006年于中科院合肥物质科学研究院获得博士学位，主要从事激光光谱高灵敏检测方法与应用技术的研究。E-mail：kanruifeng@aiofm.ac.cn KAN rui-feng, E-mail: kanruifeng@aiofm.ac.cn
- 基金信息：
  
  国家自然科学基金青年基金资助项目(31701512);国家自然科学基金资助项目(61772198);浙江省重点研发项目资助(2019C02013);湖州市自然科学基金资助项目(2017YZ03)
- DOI：10.3788/OPE.20192706.1397
  中图分类号： O433.1;O657.38
- 收稿日期：2019-02-25，
  
  录用日期：2019-3-20，
  
  纸质出版日期：2019-06-15
- 稿件说明：
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贾良权, 祁亨年, 胡文军, 等. 种子呼吸CO₂浓度检测系统[J]. 光学精密工程, 2019,27(6):1397-1404.

liang-quan JIA, heng-nian QI, wen-jun HU, et al. CO₂ concentration detection system for seed respiration[J]. Optics and precision engineering, 2019, 27(6): 1397-1404.
贾良权, 祁亨年, 胡文军, 等. 种子呼吸CO₂浓度检测系统[J]. 光学精密工程, 2019,27(6):1397-1404. DOI： 10.3788/OPE.20192706.1397.

liang-quan JIA, heng-nian QI, wen-jun HU, et al. CO₂ concentration detection system for seed respiration[J]. Optics and precision engineering, 2019, 27(6): 1397-1404. DOI： 10.3788/OPE.20192706.1397.

摘要

针对传统种子呼吸CO

浓度检测方法中检测精度低的问题，为了满足测量需要，提出一种采用可调谐二极管激光器吸收光谱技术的种子呼吸测量系统方案。该系统是由多次反射池结构的种子呼吸容器、分布反馈式激光器及其控制电路、光电转换及放大电路、数据采集电路、上位机软件等构成，设计种子呼吸容器其空间体积为1.5 L，激光器光源采用2 004 nm波段，多次反射池光程为16 m。然后，基于朗伯比尔定律，通过波长调制吸收光谱技术，利用二次谐波实时反演出种子呼吸过程中产生CO

气体的浓度。测试结果显示：种子呼吸CO

浓度测量的稳定重复性为0.033%，CO

浓度的线性拟合度为0.999 38，CO

浓度检测极限为1.7 ppm。通过实验对糯玉米种子进行检测，获得20 g玉米种子呼吸的变化曲线，其12 h内变化量为2 750.5 ppm，呼吸速率为229.2 ppm/h，实验结果表明该系统能解决种子呼吸CO

浓度无法连续性测量、浓度检测精度低等问题。

Abstract

To address the problem of low detection accuracy in the traditional method of seed-breathing CO

concentration measurement

a seed-breathing measurement system based on tunable diode laser absorption spectroscopic technology was proposed to meet the needs of seed-breathing CO

concentration measurements. First

the system was designed to consist of a seed-breathing container

a distributed feedback laser and control circuit

photoelectric conversion and an amplification circuit

a data acquisition circuit

and upper computer software. The space volume of the seed breathing container was 1.5 L

the laser source was in the 2 004-nm band

and the light path of the multiple reflection cell was 16 m. Then

based on Lambert's law and wavelength modulated absorption spectroscopy

the concentration of CO

produced during seed respiration could be retrieved in real time using second harmonics. The stable repeatability of the CO

concentration measurement in seed respiration is 0.033%

the linear fitting degree of CO

concentration is 0.999 38

and the detection limit of CO

concentration is 1.7×10

-6

. The change curve of 20-g maize seed respiration is obtained by testing waxy maize seeds. The amount of change in waxy maize seed respiration after 12 h is 2 750.5×10

-6

and the respiration rate is 229.2×10

-6

/h. Experimental results show that the system can solve the inability for continuous measurement of CO

concentration in seed respiration and the low precision of concentration detection.

关键词

Keywords

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种子呼吸CO2浓度检测系统设计

种子呼吸CO2浓度检测系统

CO2 concentration detection system for seed respiration

DOI：10.3788/OPE.20192706.1397

摘要

Abstract

关键词

Keywords

references

种子呼吸CO₂浓度检测系统

CO₂ concentration detection system for seed respiration