用于检测多巴胺的荧光增强型适体传感器

姜利英; 杭欣欣; 张培; 任林娇; 王慰

doi:10.3788/OPE.20192709.1943

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用于检测多巴胺的荧光增强型适体传感器

微纳技术与精密机械 | 更新时间：2020-08-13

- 用于检测多巴胺的荧光增强型适体传感器
- Fluorescence-enhanced aptamer sensor for dopamine detection
- 光学精密工程 2019年27卷第9期页码：1943-1949
- 作者机构：
  
  郑州轻工业大学电气信息工程学院，河南郑州 450000
- 作者简介：
  
  [ "姜利英 (1981-)，女，河南郾城人，博士，教授，2007年于中国科学院电子学研究所获得博士学位，主要从事生物传感器及检测微系统方面的研究。E-mail:jiangliying@zzuli.edu.cn" ]
  任林娇 (1987-)，河南济源人，博士，讲师，2010年、2015年于重庆大学分别获得学士、博士学位，主要从事特种荧光材料制备与生物荧光传感检测相关领域的科研工作。E-mail:renlinjiao@zzuli.edu.cn REN Lin-jiao, E-mail:renlinjiao@zzuli.edu.cn
  王慰 (1969-)，男，博士，教授，2010年入选国家千人计划，成为国家特聘专家。2011-2017年担任中科院物联网中心的传感器研究室主任和北京印刷学院首席科学家，从事计算机视觉与人工智能，触觉感知与人工智能，智能传感器设计等方面的学术研究，并致力于人工智能、智能传感器、创客教育等技术的产业化。E-mail: 18900616029@189.cn WANG Wei, E-mail: 18900616029@189.cn
- 基金信息：
  
  国家自然科学基金资助项目(61801436);河南省科技创新人才计划项目(184200510015);河南省高等学校重点科研项目(18A510021);河南省科技计划项目-国际合作项目(182102410093)
- DOI：10.3788/OPE.20192709.1943
  中图分类号： O657.3; TP212.3
- 收稿日期：2019-05-28，
  
  录用日期：2019-7-12，
  
  纸质出版日期：2019-09-15
- 稿件说明：
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姜利英, 杭欣欣, 张培, 等. 用于检测多巴胺的荧光增强型适体传感器[J]. 光学精密工程, 2019,27(9):1943-1949.

Li-ying JIANG, Xin-xin HANG, Pei ZHANG, et al. Fluorescence-enhanced aptamer sensor for dopamine detection[J]. Optics and precision engineering, 2019, 27(9): 1943-1949.
姜利英, 杭欣欣, 张培, 等. 用于检测多巴胺的荧光增强型适体传感器[J]. 光学精密工程, 2019,27(9):1943-1949. DOI： 10.3788/OPE.20192709.1943.

Li-ying JIANG, Xin-xin HANG, Pei ZHANG, et al. Fluorescence-enhanced aptamer sensor for dopamine detection[J]. Optics and precision engineering, 2019, 27(9): 1943-1949. DOI： 10.3788/OPE.20192709.1943.

摘要

利用贵金属纳米颗粒独特的物理特性，设计具有信号放大功能的荧光适体传感器用于多巴胺的浓度检测。基于金属荧光增强效应通过在金纳米颗粒与荧光基团之间添加隔离层的手段实现荧光信号放大。将化学修饰了SH键的核酸适体与金纳米颗粒溶液混合，形成稳定的Au-S键结构并与标记荧光基团的DNA互补链利用碱基互补配对原则结合。然后，通过调节所设计的核酸适体5′所添加的碱基A的数量，从而调节荧光基团与金纳米颗粒表面的距离。同时，优化核酸适体与金纳米颗粒之间的浓度比以及所处的反应环境的pH值，获得最佳的放大效率。最后对不同浓度的多巴胺进行测试。实验结果表明：金纳米颗粒溶液与核酸适体在一定的浓度比之下，在隔离层厚度为27个碱基A时，最大的荧光增强倍数为2.35。多巴胺浓度检测的线性范围为20~100 nmol/L

最低检测限为20 nmol/L。该传感器可以在纳米级有效调控隔离层厚度，提供了一种稳定的信号放大策略。

Abstract

Based on the unique physical properties of noble metal nanoparticles

a fluorescence sensor with signal amplification function was designed for detecting the concentration of dopamine. Based on the effect of metal fluorescence enhancement

the fluorescence signal was amplified by adding a spacer layer between the gold nanoparticles and fluorophore. First

the aptamer chemically modified with an SH bond was mixed with a gold nanoparticle solution to form a stable Au-S bond structure. Then

the DNA labeled with fluorescent groups were bound due to the complementary base pairing. The distance between the fluorophore and the surface of the gold nanoparticle was adjusted by changing the amount of base A. At the same time

the concentration ratio between the aptamer and gold nanoparticles and the pH of the reaction environment were optimized to obtain the best amplification efficiency. Finally

different concentrations of dopamine were tested. The experimental results indicate that at a certain concentration ratio between the gold nanoparticle solution and aptamer

the maximum fluorescence enhancement is 2.35 times at the isolation layer thickness is 27 base A. Dopamine concentration detection has a linear range of 20-100 nmol/L. The detection limit is 20 nmol/L. It can effectively regulate the isolation layer thickness at the nanometer level

providing a stable signal amplification strategy.

关键词

Keywords

references

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